Anti-oxidative effect of theβ-blocker carvedilol on diabetic nephropathy in non-obese type 2 diabetic rats

Cladophora glomerataextract (CGE) has been shown to exhibit antigastric ulcer, anti-inflammatory, analgesic, hypotensive, and antioxidant activities. The present study investigated antidiabetic and renoprotective effects of CGE in type 2 diabetes mellitus (T2DM) rats. The rats were induced by high-fat diet and streptozotocin and supplemented daily with 1 g/kg BW of CGE for 12 weeks. The renal transport function was assessed by the uptake ofpara-aminohippurate mediated organic anion transporters 1 (Oat1) and 3 (Oat3), using renal cortical slices. These two transporters were known to be upregulated by insulin and PKCζwhile they were downregulated by PKCαactivation. Compared to T2DM, CGE supplemented rats had significantly improved hyperglycaemia, hypertriglyceridemia, insulin resistance, and renal morphology. The baseline uptake ofpara-aminohippurate was not different among experimental groups and was correlated with Oat1 and 3 mRNA expressions. Nevertheless, while insulin-stimulated Oat1 and 3 functions in renal slices were blunted in T2DM rats, they were improved by CGE supplementation. The mechanism of CGE-restored insulin-stimulated Oat1 and 3 functions was clearly shown to be associated with upregulated PKCζand downregulated PKCαexpressions and activations. These findings indicate that CGE has antidiabetic effect and suggest it may prevent diabetic nephropathy through PKCs in a T2DM rat model.

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Effect of pioglitazone on diabetic nephropathy and expression of HIF-1α and VEGF in the renal tissues of type 2 diabetic rats

Diabetes Research and Clinical Practice ◽

10.1016/j.diabres.2011.03.019 ◽

2011 ◽

Vol 93 (1) ◽

pp. 63-69 ◽

Cited By ~ 15

Author(s):

Xiangjin Xu ◽

Pin Chen ◽

Quanlin Zheng ◽

Yanqiao Wang ◽

Wenyu Chen

Keyword(s):

Diabetic Nephropathy ◽

Diabetic Rats ◽

Type 2 Diabetic

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Trigonelline reduced diabetic nephropathy and insulin resistance in type�2 diabetic rats through peroxisome proliferator‑activated receptor‑γ

Experimental and Therapeutic Medicine ◽

10.3892/etm.2019.7698 ◽

2019 ◽

Cited By ~ 2

Author(s):

Yinyan Li ◽

Qaobei Li ◽

Chunyan Wang ◽

Zhe Lou ◽

Qingchang Li

Keyword(s):

Insulin Resistance ◽

Diabetic Nephropathy ◽

Diabetic Rats ◽

Peroxisome Proliferator ◽

Peroxisome Proliferator Activated Receptor ◽

Type 2 Diabetic

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Hu-Lu-Ba-Wan Attenuates Diabetic Nephropathy in Type 2 Diabetic Rats through PKC-α/NADPH Oxidase Signaling Pathway

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2013/504642 ◽

2013 ◽

Vol 2013 ◽

pp. 1-10 ◽

Cited By ~ 12

Author(s):

Lishan Zhou ◽

Hui Dong ◽

Yi Huang ◽

Lijun Xu ◽

Xin Zou ◽

...

Keyword(s):

Diabetic Nephropathy ◽

Nadph Oxidase ◽

Signaling Pathway ◽

Superoxide Anion ◽

Nicotinamide Adenine Dinucleotide Phosphate ◽

Renal Tissue ◽

Diabetic Rats ◽

Control Group ◽

Type 2 Diabetic

Hu-Lu-Ba-Wan (HLBW) is a Chinese herbal prescription used to treat kidney deficiency. The aim of this study was to explore the effect and mechanism of HLBW on diabetic nephropathy (DN) in type 2 diabetic rats. The rat model of DN was established by being fed a high-fat diet and intravenous injection of streptozotocin. Then, HLBW decoction was administered for 16 weeks. Blood glucose level, lipid profile, renal function, 24-hour total urinary protein, and albumin content were examined. Renal morphology and superoxide anion levels were evaluated. The activity of nicotinamide-adenine dinucleotide phosphate (NADPH) and protein kinase C-alpha (PKC-α) related genes expression in renal tissue were also determined. Our data demonstrated that HLBW significantly improved hyperglycemia, hyperlipidemia, and proteinuria in diabetic rats compared with those of control group. HLBW also alleviated glomerular expansion and fibrosis, extracellular matrix accumulation and effacement of the foot processes. Additionally, HLBW reduced superoxide anion level, NADPH oxidase activity, the protein and mRNA expressions of p47phox, and the protein expression of phosphorylated PKC-αin renal tissue. These results suggest that HLBW is effective in the treatment of DN in rats. The underlying mechanism may be related to the attenuation of renal oxidative stress via PKC-α/NADPH oxidase signaling pathway.

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