Gomisin A modulates aging progress via mitochondrial biogenesis in human diploid fibroblast cells

2018 ◽  
Vol 45 (6) ◽  
pp. 547-555 ◽  
Author(s):  
Jeong-Seok Kim ◽  
Seon-Hwa Jeong ◽  
Sin-Hee Han ◽  
Ho-Keun Yi
Keyword(s):  
Mitochondrial Biogenesis ◽  
Fibroblast Cells ◽  
Human Diploid Fibroblast ◽  
Diploid Fibroblast ◽  
Gomisin A

Maintenance of human cytomegalovirus genome in human diploid fibroblast cells

Virology ◽  
1983 ◽  
Vol 130 (1) ◽  
pp. 269-271 ◽  
Author(s):  
Linda J. Bucher ◽  
Brian Wigdahl ◽  
Fred Rapp
Keyword(s):  
Human Cytomegalovirus ◽  
Fibroblast Cells ◽  
Human Diploid Fibroblast ◽  
Diploid Fibroblast

scholarly journals Improved glycine-extracted complement-fixing antigen for human cytomegalovirus

1977 ◽  
Vol 6 (6) ◽  
pp. 647-649
Author(s):  
J D Kettering ◽  
N J Schmidt ◽  
E H Lennette
Keyword(s):  
Human Cytomegalovirus ◽  
High Multiplicity ◽  
Fibroblast Cells ◽  
Human Diploid Fibroblast ◽  
Glycine Buffer ◽  
Diploid Fibroblast

High-titered, sensitive, and stable complement-fixing antigens for human cytomegalovirus were consistently produced from human diploid fibroblast cells infected at a high multiplicity of virus, harvested after 7 days of incubation, and sonically treated immediately in 0.1 M glycine buffer, pH 9.5.

scholarly journals Purification and molecular cloning of prostacyclin-stimulating factor from serum-free conditioned medium of human diploid fibroblast cells

1994 ◽  
Vol 303 (2) ◽  
pp. 591-598 ◽  
Author(s):  
T Yamauchi ◽  
F Umeda ◽  
M Masakado ◽  
M Isaji ◽  
S Mizushima ◽  
...  
Keyword(s):  
Conditioned Medium ◽  
Sequence Similarity ◽  
Reversed Phase ◽  
Affinity Column ◽  
Fibroblast Cells ◽  
Human Diploid Fibroblast ◽  
Aortic Endothelial Cells ◽  
Diploid Fibroblast ◽  
Sds Page ◽  
Stimulating Factor

We attempted to identify the factor that stimulated prostacyclin (PGI2) production using conditioned medium from cultured human diploid fibroblast cells subjected to a series of purification steps using h.p.l.c. on DEAE-5PW, Heparin-5PW, Protein-Pak 300, and an insulin-like growth factor-1 ligand affinity column. The purified prostacyclin-stimulating factor (PSF) ran as a single band with a molecular mass of 31 kDa by SDS/PAGE. Analysis of the purified PSF by C4 reversed-phase h.p.l.c. showed a single sharp peak in 31% (v/v) acetonitrile. The material was purified 8000-fold with an overall yield of about 18%. The purified PSF stimulated PGI2 production by cultured bovine aortic endothelial cells at a concentration of about 10 ng/ml; maximal stimulation was achieved at a concentration of 25 ng/ml. A cDNA coding for PSF was cloned and sequenced, revealing an apparently novel protein with no obvious sequence similarity to known proteins.

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