scholarly journals Neurotensin receptor 1 deletion decreases methamphetamine self‐administration and the associated reduction in dopamine cell firing

2019 ◽  
Vol 26 (1) ◽  
Author(s):  
Sergio Dominguez‐Lopez ◽  
Ramaswamy Sharma ◽  
Michael J. Beckstead
Keyword(s):  
Self Administration ◽  
Neurotensin Receptor ◽  
Cell Firing ◽  
Neurotensin Receptor 1 ◽  
Dopamine Cell

scholarly journals Neurotensin receptor 1 deletion suppresses methamphetamine self-administration and the associated reduction in dopamine cell firing

2019 ◽  
Author(s):  
Sergio Dominguez-Lopez ◽  
Ramaswamy Sharma ◽  
Michael J. Beckstead
Keyword(s):  
Fixed Ratio ◽  
Firing Frequency ◽  
Firing Activity ◽  
Self Administration ◽  
Population Activity ◽  
Neurotensin Receptor ◽  
Cell Firing ◽  
Neurotensin Receptor 1 ◽  
Dopamine Cell

ABSTRACTWe previously reported that pharmacological blockade of neurotensin receptors in the ventral tegmental area (VTA) decreases methamphetamine (METH) self-administration in mice. Here we explored the consequences of genetic deletion of neurotensin receptor 1 (NtsR1) in METH self-administration and VTA dopamine neuron firing activity. We implanted mice with an indwelling jugular catheter and trained them to nose-poke for intravenous infusions of METH. Mice with NtsR1 deletion (KO) acquired selfadministration similar to wildtype (WT) and heterozygous (HET) littermates. However, in NtsR1 KO and HET mice, METH intake and motivated METH seeking decreased when the response requirement was increased to a fixed ratio 3 and when mice were tested on a progressive ratio protocol. After completion of METH self-administration, single cell in vivo extracellular recordings of dopamine firing activity in the VTA were obtained in anesthetized mice. In WT METH-experienced mice, dopamine cell firing frequency dramatically decreases compared to WT drug-naïve mice. NtsR1 KO and HET mice did not exhibit this decline of dopamine cell firing activity after prolonged METH selfadministration. We also observed an increase in population activity following METH selfadministration that was strongest in the WT group. Our results suggest a role for NtsR1 in METH-seeking behavior, and ablation of NtsR1 receptors prevents the detrimental effects of prolonged METH self-administration on VTA dopamine cell firing frequency.

scholarly journals Systemic PD149163, a neurotensin receptor 1 agonist, decreases methamphetamine self-administration in DBA/2J mice without causing excessive sedation

PLoS ONE ◽  
2017 ◽  
Vol 12 (7) ◽  
pp. e0180710 ◽  
Author(s):  
Amanda L. Sharpe ◽  
Erika Varela ◽  
Michael J. Beckstead
Keyword(s):  
Self Administration ◽  
Neurotensin Receptor ◽  
Neurotensin Receptor 1 ◽  
Excessive Sedation

Effects of prefrontal cortex microinjection of neurotensin-(8-13) on midbrain dopamine and non-dopamine cell firing

2000 ◽  
Vol 876 (1-2) ◽  
pp. 196-200 ◽  
Author(s):  
Maria D Fatigati ◽  
Roberta M Anderson ◽  
Pierre-Paul Rompré
Keyword(s):  
Prefrontal Cortex ◽  
Cell Firing ◽  
Midbrain Dopamine ◽  
Dopamine Cell

Clonidine modulates dopamine cell firing in rat ventral tegmental area

1989 ◽  
Vol 165 (1) ◽  
pp. 11-18 ◽  
Author(s):  
Johan Grenhoff ◽  
Torgny H. Svensson
Keyword(s):  
Ventral Tegmental Area ◽  
Cell Firing ◽  
Ventral Tegmental ◽  
Dopamine Cell

scholarly journals Heterodimerization of apelin receptor and neurotensin receptor 1 induces phosphorylation of ERK 1/2 and cell proliferation via Gαq‐mediated mechanism

2014 ◽  
Vol 18 (10) ◽  
pp. 2071-2081 ◽  
Author(s):  
Bo Bai ◽  
Xin Cai ◽  
Yunlu Jiang ◽  
Emmanouil Karteris ◽  
Jing Chen
Keyword(s):  
Cell Proliferation ◽  
Neurotensin Receptor ◽  
Apelin Receptor ◽  
Neurotensin Receptor 1

scholarly journals Neurotensin and neurotensin receptor 1 mRNA expression in song-control regions changes during development in male zebra finches

2018 ◽  
Vol 78 (7) ◽  
pp. 671-686
Author(s):  
Devin P. Merullo ◽  
Chinweike N. Asogwa ◽  
Miguel Sanchez-Valpuesta ◽  
Shin Hayase ◽  
Bikash R. Pattnaik ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Zebra Finches ◽  
Neurotensin Receptor ◽  
Song Control ◽  
Neurotensin Receptor 1 ◽  
Control Regions

Evaluation of neurotensin receptor 1 as potential biomarker for prostate cancer theranostic use

2019 ◽  
Vol 46 (10) ◽  
pp. 2199-2207 ◽  
Author(s):  
Tingting He ◽  
Mengzhe Wang ◽  
Hui Wang ◽  
Hongpei Tan ◽  
Yongxiang Tang ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Neurotensin Receptor ◽  
Potential Biomarker ◽  
Neurotensin Receptor 1

scholarly journals Bivalent ligands promote endosomal trafficking of the dopamine D3 receptor-neurotensin receptor 1 heterodimer

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Julian Budzinski ◽  
Simone Maschauer ◽  
Hiroyuki Kobayashi ◽  
Pierre Couvineau ◽  
Hannah Vogt ◽  
...  
Keyword(s):  
Binding Affinity ◽  
Receptor Interaction ◽  
Endosomal Trafficking ◽  
Receptor Ligands ◽  
Bivalent Ligands ◽  
Neurotensin Receptor ◽  
Neuropsychiatric Diseases ◽  
Dopamine D3 ◽  
Neurotensin Receptor 1 ◽  
G Protein Coupled

AbstractBivalent ligands are composed of two pharmacophores connected by a spacer of variable size. These ligands are able to simultaneously recognize two binding sites, for example in a G protein-coupled receptor heterodimer, resulting in enhanced binding affinity. Taking advantage of previously described heterobivalent dopamine-neurotensin receptor ligands, we demonstrate specific interactions between dopamine D3 (D3R) and neurotensin receptor 1 (NTSR1), two receptors with expression in overlapping brain areas that are associated with neuropsychiatric diseases and addiction. Bivalent ligand binding to D3R-NTSR1 dimers results in picomolar binding affinity and high selectivity compared to the binding to monomeric receptors. Specificity of the ligands for the D3R-NTSR1 receptor pair over D2R-NTSR1 dimers can be achieved by a careful choice of the linker length. Bivalent ligands enhance and stabilize the receptor-receptor interaction leading to NTSR1-controlled internalization of D3R into endosomes via recruitment of β-arrestin, highlighting a potential mechanism for dimer-specific receptor trafficking and signalling.

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