Antimicrobial effectiveness of grape seed extract against Enterococcus faecalis biofilm: A Confocal Laser Scanning Microscopy analysis

2019 ◽  
Vol 46 (2) ◽  
pp. 191-196 ◽  
Author(s):  
Nicole de Mello Fiallos ◽  
Doglas Cecchin ◽  
Carolina Oliveira Lima ◽  
Raphael Hirata ◽  
Emmanuel João Nogueira Leal Silva ◽  
...  
Keyword(s):  
Confocal Laser Scanning Microscopy ◽  
Enterococcus Faecalis ◽  
Laser Scanning ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Microscopy Analysis ◽  
Antimicrobial Effectiveness

scholarly journals Grape Seed Extract as a Potential Remineralizing Agent: A Comparative in vitro Study

2012 ◽  
Vol 13 (4) ◽  
pp. 425-430 ◽  
Author(s):  
Shiny Benjamin ◽  
Roshni LNU ◽  
Sabeena Susan Thomas ◽  
Mohan Thomas Nainan
Keyword(s):  
Laser Scanning ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Confocal Laser Scanning Microscope ◽  
Seed Extract ◽  
Confocal Laser ◽  
Scanning Microscope ◽  
Caries Lesions ◽  
Calcium Glycerophosphate

ABSTRACT Objective Remineralization is an effective treatment that may stop or reverse early tooth decay. Grape seed extract (GSE) is the potential remineralizing agent under investigation. Materials and methods Sound human tooth sections were obtained from the cervical portion of the root and stored in demineralizing solution at 37°C for 96 hours to induce artificial root caries lesions. The sections were divided into four treatment groups including 6.5% grape seed extract, sodium monofluorophosphate (220 ppm) with 0.05% calcium glycerophosphate, 0.5% calcium glycerophosphate and control (no treatment). An in vitro pH cycling model was used to cycle the demineralized specimens through treatment solutions, acidic buffer and neutral buffer for 8 days at 6 cycles per day. Subsequently, they were evaluated using confocal laser scanning microscope. Data were analyzed using analysis of variance (p < 0.05). Results GSE revealed less demineralization and more remineralization compared with other groups. Conclusion GSE promotes remineralization of artificial root caries lesions. Clinical significance The search for the perfect remineralizing agent continues to this day. GSE could be a welcome addition to the remineralization armamentarium. Abbreviations and acronyms GSE: Grape seed extract; ppm: Parts per million; CaGP: Calcium glycerophosphate; CLSM: Confocal laser scanning microscope; ANOVA: Analysis of variance; PA: Proanthocyanidin; CEJ: Cementoenamel junction; mM: Millimole; CaCl2.2H2O: Calcium chloride dihydrate; KH2PO4: Potassium dehydrate phosphate; K2HPO4: Dipotassium phosphate; dH2O: Deionized water; w/v: Weight by volume; ROD: Relative optical density; nm: Nanometer; SD: Standard deviation. How to cite this article Benjamin S, Roshni, Thomas SS, Nainan MT. Grape Seed Extract as a Potential Remineralizing Agent: A Comparative in vitro Study. J Contemp Dent Pract 2012;13(4):425-430.

Confocal Laser Scanning Microscopy Is Appropriate to Detect Viability of Enterococcus faecalis in Infected Dentin

2008 ◽  
Vol 34 (10) ◽  
pp. 1198-1201 ◽  
Author(s):  
Ronald Ordinola Zapata ◽  
Clovis M. Bramante ◽  
Ivaldo Gomes de Moraes ◽  
Norberti Bernardineli ◽  
Thais Helena Gasparoto ◽  
...  
Keyword(s):  
Confocal Laser Scanning Microscopy ◽  
Enterococcus Faecalis ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser

scholarly journals Characterization of Enterococcus faecalis in different culture conditions

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Mi-Ah Kim ◽  
Vinicius Rosa ◽  
Kyung-San Min
Keyword(s):  
Enterococcus Faecalis ◽  
Laser Scanning ◽  
Colorimetric Assay ◽  
Culture Conditions ◽  
Laser Scanning Microscopy ◽  
Extracellular Dna ◽  
Confocal Laser ◽  
Qrt Pcr ◽  
Microscopy Analysis

AbstractThe aim of this study was to investigate how carbohydrates (glucose or sucrose) affect the characteristics of Enterococcus faecalis (E. faecalis) planktonic and biofilm in vitro. For this study, E. faecalis was cultured in tryptone-yeast extract broth with 0% glucose + 0% sucrose, 0.5% glucose, 1% glucose, 0.5% sucrose, or 1% sucrose. Viability of E. faecalis was examined by colony forming unit counting assays. Biofilm formation was assessed by measuring extracellular DNA (eDNA), a component of the biofilm matrix. Quantitative real-time PCR (qRT-PCR) was performed to investigate the expression of virulence-associated genes. Field emission scanning electron microscopy analysis, confocal laser scanning microscopy analysis, and crystal violet colorimetric assay were conducted to study E. faecalis biofilms. E. faecalis showed the highest viability and eDNA levels in 1% sucrose medium in biofilms. The result of qRT-PCR showed that the virulence-associated genes expressed highest in 1% sucrose-grown biofilms and in 1% glucose-grown planktonic cultures. E. faecalis showed highly aggregated biofilms and higher bacteria and exopolysaccharide (EPS) bio-volume in sucrose than in 0% glucose + 0% sucrose or glucose. The results indicate that the production of eDNA and EPS and expression of virulence-associated genes in E. faecalis are affected by the concentration of carbohydrates in biofilm or planktonic culture.

Confocal Laser Scanning Microscopy Analysis of 10 Cases of Craniofacial Fibrous Dysplasia

2015 ◽  
Vol 39 (4) ◽  
pp. 231-234 ◽  
Author(s):  
Luigi Laino ◽  
Gianfranco Favia ◽  
Dardo Menditti ◽  
Francesco De Francesco ◽  
Carmen Salerno ◽  
...  
Keyword(s):  
Fibrous Dysplasia ◽  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser ◽  
Microscopy Analysis
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