Prolonged incubation of processed human spermatozoa will increase DNA fragmentation

Andrologia ◽  
2013 ◽  
Vol 46 (4) ◽  
pp. 374-379 ◽  
Author(s):  
A. Nabi ◽  
M. A. Khalili ◽  
I. Halvaei ◽  
F. Roodbari
Keyword(s):  
Dna Fragmentation ◽  
Human Spermatozoa ◽  
Prolonged Incubation

scholarly journals Potential effect of tobacco cigarettes smoking on global DNA methylation status and protamines transcripts in human spermatozoa

2021 ◽  
Vol 26 (1) ◽  
Author(s):  
Mohammed M. Laqqan ◽  
Maged M. Yassin
Keyword(s):  
Dna Methylation ◽  
Cigarette Smoking ◽  
Dna Fragmentation ◽  
Methylation Status ◽  
Human Spermatozoa ◽  
Global Dna Methylation ◽  
Transcription Level ◽  
Heavy Smokers ◽  
Protamine 1 ◽  
Protamine 2

Abstract Background Epigenetics refers to an alteration in gene expression without alteration in the sequence of DNA and this process may be affected by environmental factors and lifestyle like cigarette smoking. This study was designed to evaluate the potential effect of cigarette smoking on the global DNA methylation status and the transcription level of protamine 1 and protamine 2 in human spermatozoa. A total of 188 semen samples were collected from men with a mean age of 34.9 ± 5.8 years old (98 heavy smokers and 90 non-smokers). The DNA and RNA were isolated from purified spermatozoa, then the status of global DNA methylation and the transcription level of protamine 1 and protamine 2 were evaluated using ELISA and qPCR, respectively. The chromatin non-condensation and DNA fragmentation in human spermatozoa were evaluated using chromomycin A3 staining and TUNEL assay, respectively. Results A significant increase has been found in the status of global DNA methylation in spermatozoa of heavy smokers compared to non-smokers (7.69 ± 0.69 ng/μl vs. 4.90 ± 0.40 ng/μl, P < 0.001). Additionally, a significant reduction has been found in transcription level of protamine 1 (25.49 ± 0.31 vs. 23.94 ± 0.40, P < 0.001) and protamine 2 (28.27 ± 0.39 vs. 23.45 ± 0.30, P < 0.001) in heavy smokers. A downregulation has been found in the transcription level of protamine 1 and protamine 2 with a fold change of 0.497 and 0.047, respectively. A significant increase has been shown in the level of DNA fragmentation and chromatin non-condensation in heavy smokers compared to non-smokers (P < 0.001). On the other hand, a significant positive correlation has been found between sperm chromatin non-condensation, sperm DNA fragmentation, transcription level of protamine 1, transcription level of protamine 2, and global DNA methylation status (r = 0.304, P < 0.001; r = 0.399, P < 0.001; r = 0.216, P = 0.003; r = 0.494, P < 0.001, respectively). Conclusion Tobacco cigarette smoking has a potential influence on the global DNA methylation and the transcription level of protamine genes in human spermatozoa, and consequently, affect negatively on the semen parameters.

scholarly journals Testicular human spermatozoa cryopreservation correlation between sperm head vacuoles, DNA fragmentation and mitochondrial membrane potential

2018 ◽  
Vol 23 (4) ◽  
pp. 413-417
Author(s):  
Sahabeh Etebary ◽  
Nahid Yari ◽  
Mohammad Ali Khalili ◽  
Seyed Mehdi Kalantar ◽  
Morteza Anvari
Keyword(s):  
Membrane Potential ◽  
Dna Fragmentation ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Sperm Head ◽  
Human Spermatozoa

scholarly journals Aqueous leaf extract of Moringa oleifera reduced intracellular ROS production, DNA fragmentation and acrosome reaction in Human spermatozoa in vitro

Andrologia ◽  
2020 ◽  
Author(s):  
Faith T. Moichela ◽  
Gbenga A. Adefolaju ◽  
Ralf R. Henkel ◽  
Chinyerum S. Opuwari
Keyword(s):  
Dna Fragmentation ◽  
Leaf Extract ◽  
Acrosome Reaction ◽  
Moringa Oleifera ◽  
Human Spermatozoa ◽  
Ros Production ◽  
Intracellular Ros ◽  
Aqueous Leaf Extract

scholarly journals DNA fragmentation of human spermatozoa: Simple assessment of single‐ and double‐strand DNA breaks and their respective dynamic behavioral response

Andrology ◽  
2020 ◽  
Vol 8 (5) ◽  
pp. 1287-1303
Author(s):  
Ana Tímermans ◽  
Rosana Vázquez ◽  
Fátima Otero ◽  
Jaime Gosálvez ◽  
Stephen Johnston ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Behavioral Response ◽  
Human Spermatozoa ◽  
Dna Breaks ◽  
Double Strand Dna Breaks ◽  
Double Strand Dna

scholarly journals Differences in nuclear DNA fragmentation and mitochondrial integrity of semen and prepared human spermatozoa

2000 ◽  
Vol 15 (7) ◽  
pp. 1552-1561 ◽  
Author(s):  
Eilish T. Donnelly ◽  
Michael O'Connell ◽  
Neil McClure ◽  
Sheena E.M. Lewis
Keyword(s):  
Dna Fragmentation ◽  
Nuclear Dna ◽  
Human Spermatozoa ◽  
Mitochondrial Integrity ◽  
Nuclear Dna Fragmentation

Origin and biological significance of DNA fragmentation in human spermatozoa

10.2741/1898 ◽  
2006 ◽  
Vol 11 (1) ◽  
pp. 1491 ◽  
Author(s):  
Monica Muratori
Keyword(s):  
Dna Fragmentation ◽  
Biological Significance ◽  
Human Spermatozoa

scholarly journals Low rates of DNA fragmentation in selected motile human spermatozoa assessed by the TUNEL assay

2001 ◽  
Vol 16 (8) ◽  
pp. 1703-1707 ◽  
Author(s):  
L. Ramos
Keyword(s):  
Dna Fragmentation ◽  
Tunel Assay ◽  
Human Spermatozoa

In vitroincubation of human spermatozoa promotes reactive oxygen species generation and DNA fragmentation

Andrologia ◽  
2014 ◽  
pp. n/a-n/a ◽  
Author(s):  
J. Cicaré ◽  
A. Caille ◽  
C. Zumoffen ◽  
S. Ghersevich ◽  
L. Bahamondes ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Dna Fragmentation ◽  
Reactive Oxygen Species Generation ◽  
Reactive Oxygen ◽  
Human Spermatozoa ◽  
Oxygen Species

scholarly journals Beneficial effects of hypotaurine supplementation in preparation and freezing media on human sperm cryo-capacitation and DNA quality

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Hanae Pons-Rejraji ◽  
Solène Vorilhon ◽  
Asmaa Difrane ◽  
Sandra Dollet ◽  
Céline Bourgne ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Gradient Centrifugation ◽  
Human Sperm ◽  
Sperm Nucleus ◽  
Human Spermatozoa ◽  
Slow Freezing ◽  
Sperm Chromatin ◽  
Protein Markers ◽  
Chromatin Decondensation ◽  
Beneficial Effects

Abstract Background Although widely used, slow freezing considerably modifies the functions of human spermatozoa. Cryopreservation induces nuclear sperm alterations and cryo-capacitation, reducing the chances of pregnancy. Hypotaurine is naturally present in the male and female genital tracts and has capacitating, osmolytic and anti-oxidant properties. The analysis were performed on surplus semen of men with normal (n = 19) or abnormal (n = 14) sperm parameters. Spermatozoa were selected by density gradient centrifugation before slow freezing. For each sample, these steps were performed in parallel with (“H+” arm) or without (“H-” arm) hypotaurine supplementation. After thawing, we measured total and progressive mobility, vitality, acrosome integrity, markers of capacitation signaling pathway and nuclear quality. For the latter, we focused on sperm chromatin packaging, DNA fragmentation and the presence of vacuoles in the sperm nucleus. Results Post-thaw spermatozoa selected and frozen in the presence of hypotaurine had a higher vitality (+ 16.7%, p < 0.001), progressive and total motility (+ 39.9% and +  21.6% respectively, p < 0.005) than spermatozoa from the control “H-” arm. Hypotaurine also reduced the non-specific phosphorylation of the capacitation protein markers P110 and P80 (p < 0.01), indicating a decrease in cryo-capacitation. Hypotaurine supplementation reduced chromatin decondensation, measured by chromomycin A3 (− 16.1%, p < 0.05), DNA fragmentation (− 18.7%, p < 0.05) and nuclear vacuolization (− 20.8%, p < 0.05). Conclusion Our study is the first to demonstrate beneficial effects of hypotaurine supplementation in preparation and freezing procedures on human spermatozoa sperm fertilization capacity and nucleus quality. Hypotaurine supplementation limited cryo-capacitation, increased the proportion of live and progressively motile spermatozoa and reduces the percentage of spermatozoa showing chromatin decondensation, DNA fragmentation and nuclear vacuolation. Trial registration Clinical Trial, NCT04011813. Registered 19 May 2019 - Retrospectively registered.

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