Lectin-binding pattern of glycoconjugates during spontaneous testicular recrudescence in Syrian hamster (Mesocricetus auratus) after exposure to short photoperiod

Andrologia ◽  
2018 ◽  
Vol 51 (1) ◽  
pp. e13148 ◽  
Author(s):  
Jesús Martínez-Hernández ◽  
Vicente Seco-Rovira ◽  
Ester Beltrán-Frutos ◽  
Concepción Ferrer ◽  
María Isabel Serrano-Sánchez ◽  
...  
Keyword(s):  
Syrian Hamster ◽  
Lectin Binding ◽  
Mesocricetus Auratus ◽  
Short Photoperiod ◽  
Binding Pattern ◽  
Testicular Recrudescence

Proliferation, apoptosis, and number of Sertoli cells in the Syrian hamster during recrudescence after exposure to short photoperiod†‡

2019 ◽  
Vol 102 (3) ◽  
pp. 588-597 ◽  
Author(s):  
Jesús Martínez-Hernández ◽  
Vicente Seco-Rovira ◽  
Ester Beltrán-Frutos ◽  
Concepción Ferrer ◽  
María Isabel Serrano-Sánchez ◽  
...  
Keyword(s):  
Syrian Hamster ◽  
Normal Population ◽  
Similar Rate ◽  
The Other ◽  
Short Photoperiod ◽  
Control Group ◽  
Bright Field ◽  
Testicular Regression ◽  
Testicular Recrudescence ◽  
Long Photoperiod

Abstract The Sertoli cell (Sc) has been described as a quiescent cell once the animal has reached sexual maturity. Syrian hamster is an animal that displays testicular regression due to short photoperiod, during which process germ cells and Sc are removed through apoptosis. The aim of this work was to investigate histochemically whether the spontaneous testicular recrudescence processes after exposure to a short photoperiod lead to an increase in Sc proliferative activity in order to restore the normal population. Three spontaneous recrudescence groups were established: initial (IR), advanced (AR), and total (TR) recrudescence, which were compared with animal undergoing the regression process (mild: MRg, strong: SRg, and total: TRg) and animals in long photoperiod (Controls). Histological sections were submitted to histochemical techniques for detecting apoptotic and proliferative Sc with bright-field and fluorescence microscopy. For each group, the proliferative Sc index (PScI) and apoptotic Sc index (AScI), and the total number of Sc were obtained. The results revealed the existence of Vimentin+/TUNEL+ as well as Vimentin+/PCNA+ cells. The PScI was significantly higher in TRg and IR than in the other groups. The AScI was only significantly higher in MRg and SRg with respect to the other groups. The total number of Sc increased among TRg, IR, and AR, reaching values similar to those of the Controls. In conclusion, the increase in Sc proliferation from final regression and recrudescence, accompanied by a similar rate of apoptosis to the Control group, is the cause of the restoration of the Sc population during spontaneous recrudescence.

scholarly journals Testicular histomorphometry and the proliferative and apoptotic activities of the seminiferous epithelium in Syrian hamster (Mesocricetus auratus) during regression owing to short photoperiod

Andrology ◽  
2015 ◽  
Vol 3 (3) ◽  
pp. 598-610 ◽  
Author(s):  
V. Seco-Rovira ◽  
E. Beltrán-Frutos ◽  
C. Ferrer ◽  
F. J. Saez ◽  
J. F. Madrid ◽  
...  
Keyword(s):  
Syrian Hamster ◽  
Mesocricetus Auratus ◽  
Seminiferous Epithelium ◽  
Short Photoperiod

Lectin Histochemistry as a Tool to Identify Apoptotic Cells in the Seminiferous Epithelium of Syrian Hamster (Mesocricetus auratus) Subjected to Short Photoperiod

2013 ◽  
Vol 48 (6) ◽  
pp. 974-983 ◽  
Author(s):  
V Seco-Rovira ◽  
E Beltrán-Frutos ◽  
C Ferrer ◽  
MM Sánchez-Huertas ◽  
JF Madrid ◽  
...  
Keyword(s):  
Syrian Hamster ◽  
Lectin Histochemistry ◽  
Mesocricetus Auratus ◽  
Seminiferous Epithelium ◽  
Short Photoperiod ◽  
Apoptotic Cells

scholarly journals The Death of Sertoli Cells and the Capacity to Phagocytize Elongated Spermatids During Testicular Regression due to Short Photoperiod in Syrian Hamster (Mesocricetus auratus)1

2014 ◽  
Vol 90 (5) ◽  
Author(s):  
Vicente Seco-Rovira ◽  
Esther Beltrán-Frutos ◽  
Concepción Ferrer ◽  
Francisco José Sáez ◽  
Juan Francisco Madrid ◽  
...  
Keyword(s):  
Sertoli Cells ◽  
Syrian Hamster ◽  
Mesocricetus Auratus ◽  
Short Photoperiod ◽  
Testicular Regression

Changes in lectin-binding pattern in the digestive tract ofXenopus laevis during metamorphosis. I. Gastric region

1990 ◽  
Vol 205 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Atsuko Ishizuya-Oka ◽  
Atsumi Shimozawa
Keyword(s):  
Digestive Tract ◽  
Lectin Binding ◽  
Binding Pattern

Lectin-Binding Pattern in Ovarian Structures of Rats with Experimental Polycystic Ovaries

2013 ◽  
Vol 48 (5) ◽  
pp. 850-857 ◽  
Author(s):  
CG Barbeito ◽  
HH Ortega ◽  
V Matiller ◽  
EJ Gimeno ◽  
NR Salvetti
Keyword(s):  
Lectin Binding ◽  
Binding Pattern ◽  
Polycystic Ovaries

Age-related and photoperiodic variation of the DAZ gene family in the testis of the Syrian hamster (Mesocricetus auratus)

Zygote ◽  
2018 ◽  
Vol 26 (2) ◽  
pp. 127-134 ◽  
Author(s):  
Candela Rocío González ◽  
Luciana Moverer ◽  
Ricardo Saúl Calandra ◽  
Silvia Inés González-Calvar ◽  
Alfredo Daniel Vitullo
Keyword(s):  
Protein Expression ◽  
Gene Family ◽  
Syrian Hamster ◽  
Mesocricetus Auratus ◽  
Cell Number ◽  
The Novel ◽  
Age Related ◽  
Mrna And Protein Expression ◽  
Long Photoperiod ◽  
Daz Gene

SummaryThe Deleted in AZoospermia (DAZ) gene family regulates the development, maturation and maintenance of germ cells and spermatogenesis in mammals. The DAZ family consists of two autosomal genes, Boule and Dazl (Daz-like), and the Daz gene on chromosome Y. The aim of this study was to analyze the localization of DAZL and BOULE during testicular ontogeny of the seasonal-breeding Syrian hamster, Mesocricetus auratus. We also evaluated the testicular expression of DAZ family genes under short- or long-photoperiod conditions. In the pre-pubertal and adult testis, DAZL protein was found mainly in spermatogonia. BOULE was found in the spermatogonia from 20 days of age and during the pre-pubertal and adult period it was also detected in spermatocytes and round spermatids. DAZL and BOULE expression in spermatogonia was strictly nuclear only in 20-day-old hamsters. We also detected the novel mRNA and protein expression of BOULE in Leydig cells. In adult hamsters, Dazl expression was increased in regressed testis compared with non-regressed testis and DAZL protein expression was restricted to primary spermatocytes in regressed testis. These results show that DAZL and BOULE are expressed in spermatogonia at early stages in the Syrian hamster, then both proteins translocate to the cytoplasm when meiosis starts. In the adult regressed testis, the absence of DAZL in spermatogonia might be related to the decrease in germ cell number, suggesting that DAZ gene family expression is involved in changes in seminiferous epithelium during photoregression.

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