Identification of internal control genes in milk-derived mammary epithelial cells during lactation cycle of Indian zebu cow

2016 ◽  
Vol 87 (3) ◽  
pp. 344-353 ◽  
Author(s):  
Pradeep Jatav ◽  
Monika Sodhi ◽  
Ankita Sharma ◽  
Sandeep Mann ◽  
Amit Kishore ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Internal Control ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Internal Control Genes ◽  
Lactation Cycle ◽  
Zebu Cow

scholarly journals The p100 coactivator is present in the nuclei of mammary epithelial cells and its abundance is increased in response to prolactin in culture and in mammary tissue during lactation

2001 ◽  
Vol 171 (2) ◽  
pp. 329-337 ◽  
Author(s):  
MK Broadhurst ◽  
TT Wheeler
Keyword(s):  
Epithelial Cells ◽  
Cultured Cells ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Mammary Tissue ◽  
Barr Virus ◽  
Beta Casein ◽  
Epstein Barr ◽  
Lactation Cycle

The p100 coactivator, first identified as a coactivator of the Epstein-Barr virus-encoded transcription factor, EBNA-2, in cultured cells, interacts with a number of transcription factors. However, the role of p100 in animals is unclear. We found that the abundance of p100 is closely associated with the lactating state in mammary tissue of mice and cows. Using two antibodies against independent parts of the protein, p100 immunoreactivity was localised to mammary epithelial cells, and was enriched in both nuclei and endoplasmic reticulum/organelle fractions. Stimulation of beta-casein expression in cultured mammary epithelial cells was associated with an increase in abundance of the p100 protein. The relative abundance of p100 mRNA was not altered in mammary tissue throughout the gestation-lactation cycle, indicating that the abundance of p100 is altered by a post-transcriptional mechanism. Further work is required to clarify the function of p100 in mammary epithelial cells.

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