Microphysiometry to Evaluate Real-Time Response of Mammary Epithelial Cells to IGF-I

2000 ◽  
Vol 28 (1-2) ◽  
pp. 209-211 ◽  
Author(s):  
Rose Marie Robinson ◽  
R. Michael Akers ◽  
Kimberly E. Forsten
Keyword(s):  
Epithelial Cells ◽  
Real Time ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Time Response ◽  
Igf I

Stimulation of DNA synthesis in cultures of ovine mammary epithelial cells by insulin and insulin-like growth factors

1989 ◽  
Vol 123 (2) ◽  
pp. 319-326 ◽  
Author(s):  
S. J. Winder ◽  
A. Turvey ◽  
I. A. Forsyth
Keyword(s):  
Epithelial Cells ◽  
Dna Synthesis ◽  
Time Course ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Mammary Tissue ◽  
Type I ◽  
Type I Igf Receptor ◽  
Pregnant Sheep ◽  
Igf I

ABSTRACT Ovine mammary epithelial cell clumps (30–90 μm) were plated onto attached gels of rat tail collagen in serum-free medium. Synthesis of DNA by these cultures could be stimulated by insulin-like growth factor-I (IGF-I) with a median effective dose of 5 μg/l, irrespective of stage of pregnancy. The time-course of response, however, was significantly slower in cells prepared from mammary tissue of non-pregnant and early pregnant sheep compared with sheep later in pregnancy. IGF-II had approximately 10% of the potency of IGF-I in stimulating DNA synthesis. Insulin acted over a wide concentration range and produced a maximum rate of stimulation not significantly different from that produced by IGF-I. These results are consistent with actions through the type-I IGF receptor although insulin may also act through its own receptor, possibly stimulating local IGF-I production. It is concluded that IGF-I is an important mitogen for ovine mammary epithelial cells. Journal of Endocrinology (1989) 123, 319–326

IGF-I, EGF, and sex steroids regulate autophagy in bovine mammary epithelial cells via the mTOR pathway

2009 ◽  
Vol 88 (2) ◽  
pp. 117-130 ◽  
Author(s):  
Agnieszka Sobolewska ◽  
Malgorzata Gajewska ◽  
Joanna Zarzyńska ◽  
Barbara Gajkowska ◽  
Tomasz Motyl
Keyword(s):  
Epithelial Cells ◽  
Sex Steroids ◽  
Mammary Epithelial Cells ◽  
Mtor Pathway ◽  
Mammary Epithelial ◽  
Bovine Mammary Epithelial Cells ◽  
Igf I

The effect of calcium on mammary epithelial cell proliferation and the plasminogen activating system

1999 ◽  
Vol 79 (3) ◽  
pp. 277-283 ◽  
Author(s):  
F. Cheli ◽  
B. Zavizion ◽  
O. Todoulou ◽  
I. Politis
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Extracellular Calcium ◽  
Mammary Epithelial ◽  
Bovine Mammary Epithelial Cells ◽  
Type Plasminogen Activator ◽  
Urokinase Type Plasminogen Activator ◽  
Igf I ◽  
Pai 1

The objectives of this study were to examine 1) the effect of extracellular calcium on proliferation of bovine mammary epithelial cells, 2) whether extracellular calcium regulates the mitogenic effect of insulin-like growth factor (IGF-I) and epidermal growth factor (EGF) towards mammary epithelial cells, and 3) whether the effects of calcium on growth are mediated through changes in the plasminogen activating system. The BME-UV1 cells were used as a model system. Results showed that optimal proliferation of BME-UV1 cells grown in the presence of 10% dialyzed FBCS was achieved when the culture medium was supplemented with 1–2 mmol L−1 of extracellular Ca2+. IGF-I (P<0.01) but not EGF, increased proliferation of BME-UV1 cells. Furthermore, calcium does not regulate IGF-I and EGF responsiveness of BME-UV1 cells. Northern blot analysis was performed to examine the effect of extracellular calcium on expression of urokinase-type plasminogen activator (u-PA), PA inhibitor 1 (PAI-1) and u-PA receptor (u-PAR) genes by BME-UV1 cells in culture. Results showed that calcium increased expression of all above-mentioned genes after 24 h of exposure of cells to calcium, at a time that the effect of calcium on growth was not apparent. Calcium had no effect on u-PA and u-PAR expression after 48 and 72 h of exposure of cells to calcium, at a time that the effect of calcium on growth was predominant. Calcium caused a small increase of PAI-1 expression after 48 and 72 h but this small increase is apparently of limited biological value. Key words: Mammary epithelial cells, calcium, growth factors, plasminogen activating system

The Effect of Bovine Colostrums IGF-I on Cultured Human Mammary Epithelial Cells Line HBL-100

2011 ◽  
Vol 343-344 ◽  
pp. 1064-1069
Author(s):  
Ji Xian Mo ◽  
Bo Hui Zhang ◽  
Xue Jun Gao
Keyword(s):  
Cell Growth ◽  
Epithelial Cells ◽  
Doubling Time ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Population Doubling ◽  
Population Doubling Time ◽  
Human Mammary Epithelial Cells ◽  
Igf I ◽  
Human Mammary Epithelial

This experimental studied the effect of the bovine colostrums IGF-I on the cultured human mammary epithelial cells line HBL-100. We selected the best optimal concentration of the bovine colostrums IGF-I on the HBL-100 and researched the cell growth curve, cell population doubling time and cell growth saturation density. Using HPLC to determine lactose content in order to reflect bovine colostrums IGF-I which would influence on the ability of lactation of cultured human mammary epithelial cells line HBL-100. The optimal IGF- I concentration was 200ng/ml, the minimum time of cell population doubling time was 13.8h, cell growth saturation density reached 8.602×105/ml; High concentrations of IGF-Ⅰhad inhibit trend to the HBL-100 cells; bovine colostrums IGF-I could promote the synthesis of the lactose of the HBL-100 significantly.

scholarly journals Short Communication: Intramammary Infusion of IGF-I Increases Bromodeoxyuridine Labeling in Mammary Epithelial Cells of Prepubertal Heifers

2005 ◽  
Vol 88 (8) ◽  
pp. 2771-2773 ◽  
Author(s):  
L.F.P. Silva ◽  
J.S. Liesman ◽  
B.E. Etchebarne ◽  
M.S. Weber Nielsen ◽  
M.J. VandeHaar
Keyword(s):  
Epithelial Cells ◽  
Short Communication ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Igf I

Differential effects of retinoids on proliferation of bovine mammary epithelial cells in collagen gel culture

2001 ◽  
Vol 68 (2) ◽  
pp. 157-164 ◽  
Author(s):  
STIG PURUP ◽  
SØREN KROGH JENSEN ◽  
KRIS SEJRSEN
Keyword(s):  
Cell Proliferation ◽  
Retinoic Acid ◽  
Growth Factors ◽  
Growth Factor ◽  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Collagen Gel ◽  
Mammary Epithelial ◽  
Bovine Mammary Epithelial Cells ◽  
Igf I

The effects of increasing concentrations of retinol, retinal and retinoic acid on proliferation of bovine mammary epithelial cells were investigated in collagen gel cultures. All retinoids significantly inhibited proliferation of mammary epithelial cells. The relative inhibitory potency of the retinoids was: retinoic acid > retinal > retinol. Maximal inhibition at 10 μg/ml corresponded to a 75–95% inhibition of proliferation obtained in basal medium. Retinol, retinal and retinoic acid also inhibited proliferation of cells growth-stimulated with insulin-like growth factor-I (IGF-I). Retinoids in highest concentrations (10 μg/ml) inhibited 68–85% of proliferation of cells obtained in culture medium containing 25 ng IGF-I/ml. Retinol and retinoic acid also inhibited proliferation of cells growth-stimulated by insulin and other growth factors from the IGF growth factor family (des(1-3)IGF-I and IGF-II), as well as growth factors from the epidermal growth factor family (EGF and TGF-α), with retinoic acid being more effective than retinol. At a concentration of 100 ng/ml, retinol and retinoic acid inhibited respectively 24–38 and 44–52% of mammary cell proliferation stimulated by growth factors of the IGF family, and at 10000 ng/ml, 61–71% of cell proliferation was inhibited. The growth-stimulating effect of insulin, EGF and TGF-α was inhibited 42–64% by retinol and retinoic acid at 100 ng/ml, and 64–84% at 10000 ng/ml. The present results show that retinol, retinal and retinoic acid are potent inhibitors of bovine mammary epithelial cell proliferation. It is suggested that retinoids may have concentration-dependent roles in regulation of pubertal mammary growth and development, indicating that the milk yield potential of heifers may be affected by vitamin A status.

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