Primary ridge augmentation with collagenated xenogenic block bone substitute in combination with collagen membrane and rhBMP-2: a pilot histological investigation

Abstract Background The purpose of this study was to evaluate the volumetric changes in partially edentulous alveolar ridges augmented with customized titanium ridge augmentation matrices (CTRAM), freeze-dried bone allograft, and a resorbable collagen membrane. Methods A pre-surgical cone beam computed tomography (CBCT) scan was obtained for CTRAM design/fabrication and to evaluate pre-surgical ridge dimensions. Ridge augmentation surgery using CTRAM, freeze-dried bone allograft, and a resorbable collagen membrane was performed at each deficient site. Clinical measurements of the area of augmentation were made at the time of CTRAM placement and re-entry, and a 2nd CBCT scan 7 months after graft placement was used for volumetric analysis. Locations of each CTRAM in situ were also compared to their planned positions. Re-entry surgery and implant placement was performed 8 months after CTRAM placement. Results Nine subjects were treated with CTRAM and freeze-dried bone allograft. Four out of the nine patients enrolled (44.4%) experienced premature CTRAM exposure during healing, and in two of these cases, CTRAM were removed early. Early exposure did not result in total graft failure in any case. Mean volumetric bone gain was 85.5 ± 30.9% of planned augmentation volume (61.3 ± 33.6% in subjects with premature CTRAM exposure vs. 104.9% for subjects without premature exposure, p = 0.03). Mean horizontal augmentation (measured clinically) was 3.02 mm, and vertical augmentation 2.86 mm. Mean surgical positional deviation of CTRAM from the planned location was 1.09 mm. Conclusion The use of CTRAM in conjunction with bone graft and a collagen membrane resulted in vertical and horizontal bone gain suitable for implant placement.

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Comparison of collagen membrane and bone substitute as a carrier for rhBMP-2 in lateral onlay graft

Clinical Oral Implants Research ◽

10.1111/clr.12320 ◽

2013 ◽

Vol 26 (1) ◽

pp. e13-e19 ◽

Cited By ~ 17

Author(s):

Yun-Young Chang ◽

Jung-Seok Lee ◽

Min-Soo Kim ◽

Seong-Ho Choi ◽

Jung-Kiu Chai ◽

...

Keyword(s):

Bone Substitute ◽

Collagen Membrane ◽

Onlay Graft

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Comparison of Calcium Sulfate and Bovine Collagen Barriers for Alveolar Ridge Augmentation

Journal of Contemporary Dentistry ◽

10.5005/jp-journals-10031-1083 ◽

2014 ◽

Vol 4 (3) ◽

pp. 129-138

Author(s):

Robert Spears ◽

Ibtisam Al-Hashimi ◽

Eric S Solomon ◽

David G Kerns ◽

William W Hallmon ◽

...

Keyword(s):

Bone Graft ◽

Calcium Sulfate ◽

Autogenous Bone ◽

Collagen Membrane ◽

Alveolar Ridge ◽

Bone Augmentation ◽

Alveolar Ridge Augmentation ◽

Ridge Augmentation ◽

Bovine Collagen ◽

Bone Gain

ABSTRACT Calcium sulfate is a biologically compatible osteoconductive graft material that binds underlying bone graft and provide space maintenance. The purpose of this study was to evaluate calcium sulfate as a barrier compared to a collagen membrane for augmentation of a standardized surgically created ridge defect. For this purpose, bilateral extraction of mandibular premolars was performed on six foxhounds (Canis familiaris). Eight weeks later, a standardized osseous ridge defects (24 total) were created using a 6 mm trephine. The study was approved by the Institutional Animal Care and Use Committee (IACUC) at Baylor College of Dentistry. The osseous defects were allocated into three groups (8 defects each): group 1 received autogenous bone graft covered with collagen membrane (CM); group 2 received autogenous bone graft covered with calcium sulfate barrier (CS), and group 3 was used as control and did not receive bone augmentation and was used as control. The animals were sacrificed after 12 weeks following bone augmentation and sites were evaluated histologically for total ridge width, percentage of bone gain and cortical bone thickness. Results All sites exhibited bony fill within the defect. Analysis of variance did not reveal statistically significant difference in the mean total bone gain among CM, CS, and control groups (12.2,11.6, and 11.9) mm2, respectively, p = 0.875. Conclusion Calcium sulfate does not appear to improve bone regeneration in an osseous defect. How to cite this article Heaton ML, Kerns DG, Hallmon WW, Kessler HP, Spears R, Solomon ES, Al-Hashimi I. Comparison of Calcium Sulfate and Bovine Collagen Barriers for Alveolar Ridge Augmentation. J Contemp Dent 2014;4(3):129-138.

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Bone Augmentation of Peri-Implant Dehiscence Defects Using Multilaminated Small Intestinal Submucosa as a Barrier Membrane: An Experimental Study in Dogs

BioMed Research International ◽

10.1155/2019/8962730 ◽

2019 ◽

Vol 2019 ◽

pp. 1-11

Author(s):

Siwen Wang ◽

Weiyi Wu ◽

Yuhua Liu ◽

Xinzhi Wang ◽

Lin Tang ◽

...

Keyword(s):

Bone Regeneration ◽

Bone Substitute ◽

Small Intestinal Submucosa ◽

Control Group ◽

Collagen Membrane ◽

Micro Ct ◽

Bone Substitute Material ◽

Substitute Material ◽

Small Intestinal ◽

Intestinal Submucosa

Objective. The aim of the study is to evaluate the effects of multilaminated small intestinal submucosa (mSIS) combined with bone substitute material to repair peri-implant defects during guided bone regeneration procedures. Methods. Twelve implants were placed in bilateral lower premolars of three beagle dogs, and a peri-implant buccal bone defect (3 mm width and 4 mm height) was created at each implant site. A total of 12 sites were filled with a particulate bone substitute material and then randomly divided into three treatment groups: covered by mSIS membrane (mSIS group), covered by collagen membrane (BG group), and no treatment (control group), each group of four sites. After 12 weeks of healing, all of the animals were euthanized and dissected blocks were obtained for micro-computed tomography (micro-CT) and histological analyses. Results. Micro-CT results revealed similar horizontal width of augmented tissue and new bone formation between mSIS and BG groups (P<0.05). Histological analyses revealed that the differences in horizontal widths of newly formed bone and bone-to-implant contact between mSIS and BG groups were not significant (P>0.05). All of these parameters were significantly different from those in the control group (P<0.05). Conclusions. These findings confirmed that mSIS combined with the bone substitute material enhanced bone regeneration in peri-implant defects, in a manner similar to that of a collagen membrane.

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Combined use of xenogeneic bone substitute material covered with a native bilayer collagen membrane for alveolar ridge preservation: A randomized controlled clinical trial

Clinical Oral Implants Research ◽

10.1111/clr.13149 ◽

2018 ◽

Vol 29 (5) ◽

pp. 522-529 ◽

Cited By ~ 12

Author(s):

Ronald E. Jung ◽

Vitor M. Sapata ◽

Christoph H. F. Hämmerle ◽

Hui Wu ◽

Xiu-lian Hu ◽

...

Keyword(s):

Bone Substitute ◽

Collagen Membrane ◽

Controlled Clinical Trial ◽

Ridge Preservation ◽

Randomized Controlled Clinical Trial ◽

Bone Substitute Material ◽

Randomized Controlled ◽

Alveolar Ridge Preservation ◽

Combined Use ◽

Substitute Material

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A collagen membrane influences bone turnover marker in vivo after bone augmentation with xenogenic bone

Head & Face Medicine ◽

10.1186/s13005-020-00249-9 ◽

2020 ◽

Vol 16 (1) ◽

Author(s):

Henning Staedt ◽

Michael Dau ◽

Eik Schiegnitz ◽

Daniel G. E. Thiem ◽

Olga Tagadiuc ◽

...

Keyword(s):

Alkaline Phosphatase ◽

Acid Phosphatase ◽

Bone Turnover ◽

Bone Substitute ◽

Bone Alkaline Phosphatase ◽

Collagen Membrane ◽

Bovine Bone ◽

Total Acid ◽

Turnover Markers

Abstract Background The aim was to compare early biochemical and histological osseous healing of chronic mandibular defects regenerated with bovine bone substitute with and without collagen membrane in vivo. Methods Eight weeks after formation of a lateral full-thickness perforating bone defect in the mandible of 40 rabbits, bovine bone substitute with (“+”;n = 20) and without (“-”;n = 20) collagen membrane was applied. Blood and bone was collected 24, 72 h, 7, 14 and 21 days after surgery. Total acid phosphatase, bone acid phosphatase, total alkaline phosphatase and bone alkaline phosphatase activities were compared between groups. Formation of new bone was quantified histologically for all time points. Results Twenty-four hours after surgery, bone alkaline phosphatase was significantly elevated in “+” group when compared to “-” (p=0.012). After 72 hours, all bone turnover markers except for total acid phosphatase (p=0.078) where significantly elevated in “+” (all p < 0.05). Fourteen days after surgery, the significant highest values for all bone turnover markers were detected in “-” (all p < 0.05). A significant difference in favor of group “-” could also be detected after 3 weeks in terms of both acid phosphatases (p < 0.05). In histology, no significant differences could be detected. Conclusion Bone regeneration with bovine bone substitute material and collagen membrane shows a significantly earlier bone remodeling activity but does not seem to influence formation of new bone in histological samples.

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