A collagen membrane influences bone turnover marker in vivo after bone augmentation with xenogenic bone

Abstract Background The aim was to compare early biochemical and histological osseous healing of chronic mandibular defects regenerated with bovine bone substitute with and without collagen membrane in vivo. Methods Eight weeks after formation of a lateral full-thickness perforating bone defect in the mandible of 40 rabbits, bovine bone substitute with (“+”;n = 20) and without (“-”;n = 20) collagen membrane was applied. Blood and bone was collected 24, 72 h, 7, 14 and 21 days after surgery. Total acid phosphatase, bone acid phosphatase, total alkaline phosphatase and bone alkaline phosphatase activities were compared between groups. Formation of new bone was quantified histologically for all time points. Results Twenty-four hours after surgery, bone alkaline phosphatase was significantly elevated in “+” group when compared to “-” (p=0.012). After 72 hours, all bone turnover markers except for total acid phosphatase (p=0.078) where significantly elevated in “+” (all p < 0.05). Fourteen days after surgery, the significant highest values for all bone turnover markers were detected in “-” (all p < 0.05). A significant difference in favor of group “-” could also be detected after 3 weeks in terms of both acid phosphatases (p < 0.05). In histology, no significant differences could be detected. Conclusion Bone regeneration with bovine bone substitute material and collagen membrane shows a significantly earlier bone remodeling activity but does not seem to influence formation of new bone in histological samples.

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A collagen membrane influences bone turnover marker in vivo after bone augmentation with xenogenic bone

10.21203/rs.3.rs-48773/v3 ◽

2020 ◽

Author(s):

Henning Staedt ◽

Michael Dau ◽

Eik Schiegnitz ◽

Daniel G.E. Thiem ◽

Olga Tagadiuc ◽

...

Keyword(s):

Alkaline Phosphatase ◽

Acid Phosphatase ◽

Bone Turnover ◽

Bone Substitute ◽

Bone Alkaline Phosphatase ◽

Collagen Membrane ◽

Bovine Bone ◽

Total Acid ◽

Turnover Markers

Abstract Background: The aim was to compare early biochemical and histological osseous healing of chronic mandibular defects regenerated with bovine bone substitute with and without collagen membrane in vivo. Methods: Eight weeks after formation of a lateral full-thickness perforating bone defect in the mandible of 40 rabbits, bovine bone substitute with (“+”;n=20) and without (“-“;n=20) collagen membrane was applied. Blood and bone was collected 24, 72 hours, 7, 14 and 21 days after surgery. Total acid phosphatase, bone acid phosphatase, total alkaline phosphatase and bone alkaline phosphatase activities were compared between groups. Formation of new bone was quantified histologically for all time points.Results: Twenty-four hours after surgery, bone alkaline phosphatase was significantly elevated in “+” group when compared to “-“ (p=0.012). After 72 hours, all bone turnover markers except for total acid phosphatase (p=0.078) where significantly elevated in “+” (all p<0.05). 14 days after surgery, the significant highest values for all bone turnover markers were detected in “-“ (all p<0.05). A significant difference in favor of group “-“ could also be detected after 3 weeks in terms of both acid phosphatases (p<0.05). In histology, no significant differences could be detected.Conclusion: Bone regeneration with bovine bone substitute material and collagen membrane shows a significantly earlier bone remodeling activity but does not seem to influence formation of new bone in histological samples.

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A collagen membrane influences bone turnover marker in vivo after bone augmentation with xenogenic bone

10.21203/rs.3.rs-48773/v2 ◽

2020 ◽

Author(s):

Henning Staedt ◽

Michael Dau ◽

Eik Schiegnitz ◽

Daniel G.E. Thiem ◽

Olga Tagadiuc ◽

...

Keyword(s):

Alkaline Phosphatase ◽

Acid Phosphatase ◽

Bone Turnover ◽

Bone Substitute ◽

Bone Alkaline Phosphatase ◽

Collagen Membrane ◽

Bovine Bone ◽

Total Acid ◽

Turnover Markers

Abstract Background: The aim was to compare early biochemical and histological osseous healing of chronic mandibular defects regenerated with bovine bone substitute with and without collagen membrane in vivo.Methods: Eight weeks after formation of a lateral full-thickness two-wall defect in the mandible of 40 rabbits, bovine bone substitute with (“+”;n=20) and without (“-“;n=20) collagen membrane was applied. Blood and bone was collected 24, 72 hours, 7, 14 and 21 days after surgery. Total acid phosphatase, bone acid phosphatase, total alkaline phosphatase and bone alkaline phosphatase activities were compared between groups. Formation of new bone was quantified histologically for all time points.Results: Twenty-four hours after surgery, bone alkaline phosphatase was significantly elevated in “+” group when compared to “-“ (p=0.012). After 72 hours, all bone turnover markers except for total acid phosphatase (p=0.078) where significantly elevated in “+” (all p<0.05). 14 days after surgery, the significant highest values for all bone turnover markers were detected in “-“ (all p<0.05). A significant difference in favor of group “-“ could also be detected after 3 weeks in terms of both acid phosphatases (p<0.05). In histology, no significant differences could be detected.Conclusion: Bone regeneration with bovine bone substitute material and collagen membrane shows a significantly earlier bone remodeling activity but does not seem to influence formation of new bone in histological samples.

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A Collagen Membrane Influences Bone Turnover Marker in Vivo After Bone Augmentation With Xenogenic Bone

10.21203/rs.3.rs-48773/v1 ◽

2020 ◽

Author(s):

Henning Staedt ◽

Michael Dau ◽

Eik Schiegnitz ◽

Daniel G.E. Thiem ◽

Olga Tagadiuc ◽

...

Keyword(s):

Alkaline Phosphatase ◽

Acid Phosphatase ◽

Bone Turnover ◽

Bone Substitute ◽

Bone Alkaline Phosphatase ◽

Collagen Membrane ◽

Bovine Bone ◽

Total Acid ◽

Turnover Markers

Abstract Background:The aim was to compare early biochemical and histological osseous healing of chronic mandibular defects regenerated with bovine bone substitute with and without collagen membranein vivo. Methods:Eight weeks after formation of a lateral defect in the mandible of 40 rabbits, bovine bone substitute with (“+”;n=20) and without (“-“;n=20) collagen membrane was applied. Blood and bone was collected 24, 72 hours, 7, 14 and 21 days after surgery. Total acid phosphatase, bone acid phosphatase, total alkaline phosphatase and bone alkaline phosphatase activities were compared between groups. Formation of new bone was quantified histologically for all time points.Results:24 hours after surgery, bone alkaline phosphatase was significantly elevated in “+” group when compared to “-“ (p=0.012). After 72 hours, all bone turnover markers except for total acid phosphatase (p=0.078) where significantly elevated in “+” (all p<0.05). 14 days after surgery, the significant highest values for all bone turnover markers were detected in “-“ (all p<0.05). A significant difference in favor of group “-“ could also be detected after 3 weeks in terms of both acid phosphatases (p<0.05). In histology, no significant differences could be detected.Conclusion: Bone regeneration with bovine bone substitute material and collagen membrane shows a significantly earlier bone remodeling activity but does not seem to influence formation of new bone in histological samples.

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KOSTNYY METABOLIZM I POTERYa KOSTNOY TKANI V OTDALENNYE SROKI POSLE ALLOTRANSPLANTATsII TRUPNOY POChKI (ATP) (ODNOMOMENTNOE ISSLEDOVANIE)

Osteoporosis and Bone Diseases ◽

10.14341/osteo2005230-33 ◽

2005 ◽

Vol 8 (2) ◽

pp. 30-33

Author(s):

I P ERMAKOVA ◽

I A PRONChENKO ◽

V P BUZULINA ◽

N A TOMILINA ◽

R N VEDERNIKOVA ◽

...

Keyword(s):

Renal Failure ◽

Alkaline Phosphatase ◽

Bone Turnover ◽

Mineral Content ◽

Bone Alkaline Phosphatase ◽

Calcium Excretion ◽

High Bone Turnover ◽

High Bone ◽

Turnover Markers ◽

High Degree

Bone turnover markers [osteocalcin (OC), carboxyterminal procollagen I propeptide (PICP), bone alkaline phosphatase (bALP), deoxypyridinoline (DPD), β -crosslaps (CTX), bone acid phosphatase (bACP), parathyroid hormone (PTH)], day calcium excretion (uCaV) and lumbar and hip bone mineral content (BMC) were determined in 195 patients [78 women and 74 men with intact renal function as well as 11 women and 31 men with renal failure (RF)] 40±33 months following KT. KT recipients received triple immunosuppressive therapy (CysA, prednisolone and azathioprine). All groups showed bone remodeling dissociation namely bone resorption increase (high CTX, DPD, bACP) and bone formation retard (mild enhanced level OC, normal or low bALP) in association with high PTH, decreased uCaV and BMC levels. High bone turnover and low uCaV were much more high degree in RF. Vertebral and femur BMC decrease was mild (osteopenia) and only men with RF revealed peripheral osteoporosis. Hyperparathyroidism was associated with decreased transplant function and was main predictor of high bone turnover and bone loss after KT.

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Longitudinal Association between Sex Hormone Levels, Bone Loss, and Bone Turnover in Elderly Men

The Journal of Clinical Endocrinology & Metabolism ◽

10.1210/jc.2003-030736 ◽

2003 ◽

Vol 88 (11) ◽

pp. 5327-5333 ◽

Cited By ~ 170

Author(s):

Luigi Gennari ◽

Daniela Merlotti ◽

Giuseppe Martini ◽

Stefano Gonnelli ◽

Beatrice Franci ◽

...

Keyword(s):

Bone Mineral Density ◽

Alkaline Phosphatase ◽

Bone Loss ◽

Bone Turnover ◽

Bone Turnover Markers ◽

Bone Alkaline Phosphatase ◽

Mineral Density ◽

Elderly Men ◽

Hormone Levels ◽

Turnover Markers

Abstract Male osteoporosis is an increasingly important health problem. It is known that sex steroid hormones play an important role in regulating bone turnover and bone mass in males as well as in females. However, the exact mechanism of bone loss in men remains unknown. In the present study, 200 elderly men (age range, 55–85 yr) were followed for 4 yr to evaluate the relationships between hormone levels, bone turnover markers, bone mineral density, and rates of bone loss. Femoral and lumbar bone mineral density, bone ultrasound parameters at the os calcis, serum testosterone (T), serum estradiol (E2), SHBG levels, and bone turnover markers (urinary crosslaps and bone alkaline phosphatase) were evaluated for each man at enrollment and 4 yr afterward. The free androgen index (FAI) and free estrogen index (FEI) as well as measures of the bioavailable sex hormones [calculated bioavailable E2 (c-bioE2) and T (c-bioT)] were calculated from total hormone levels and SHBG. In the total population, T, c-bioT, c-bioE2, FAI, and FEI, but not E2, decreased significantly with age, whereas SHBG increased significantly. Subjects with FEI, c-bioE2, and E2 levels below the median showed higher rates of bone loss at the lumbar spine and the femoral neck as well as higher speed-of-sounds decrease at the calcaneus with respect to men with FEI, c-bioE2, and E2 levels above the median. Serum bone alkaline phosphatase and urinary crosslaps were significantly higher in men with FEI, c-bioE2, and E2 in the lower quartile than in men with FEI, c-bioE2, and E2 levels in the higher quartile. No statistically significant differences were observed in relation to T, c-bioT, or FAI levels. Finally, the ratio between E2 and T, an indirect measure for aromatase activity, increased significantly with age and was higher in normal than in osteoporotic subjects. In conclusion, results from the present study indicate an important role of estrogens, and particularly of the ability to aromatize T to E2, in the regulation of bone loss and bone metabolism in elderly men.

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Biochemical Markers of Calcium and Bone Metabolism during 18 Months of Lactation in Gambian Women Accustomed to a Low Calcium Intake and in Those Consuming a Calcium Supplement1

The Journal of Clinical Endocrinology & Metabolism ◽

10.1210/jcem.83.4.4737 ◽

1998 ◽

Vol 83 (4) ◽

pp. 1059-1066

Author(s):

Ann Prentice ◽

Landing M. A. Jarjou ◽

Dorothy M. Stirling ◽

Rochelle Buffenstein ◽

Susan Fairweather-Tait

Keyword(s):

Alkaline Phosphatase ◽

Bone Turnover ◽

Bone Metabolism ◽

Calcium Intake ◽

Calcium Supplementation ◽

Bone Alkaline Phosphatase ◽

Dihydroxyvitamin D ◽

1,25 Dihydroxyvitamin D ◽

Low Calcium ◽

Turnover Markers

The effect of 18 months of lactation on indexes of calcium and bone metabolism was studied in 60 Gambian women accustomed to a very low calcium intake. Half the women consumed a calcium supplement from 10 days postpartum for 52 weeks (supplement, 714 mg Ca/day; total Ca intake, 992 ± 114 mg/day), and half consumed placebo (total Ca intake, 288 ± 128 mg/day). Fasting blood and 24-h urine samples were collected at 1.5, 13, 52, and 78 weeks of lactation and analyzed for calciotropic hormones (intact PTH, 1,25-dihydroxyvitamin D, and calcitonin), bone turnover markers (osteocalcin, bone alkaline phosphatase, and urinary deoxypyridinoline), and plasma minerals (calcium and phosphate). The first months of lactation were associated with increased bone turnover and plasma phosphate, and decreased PTH and 1,25-dihydroxyvitamin D. These effects diminished by 52 weeks, although breast milk volumes remained high. The Gambians had higher PTH, 1,25-dihydroxyvitamin D, and bone formation than British women with a greater customary calcium intake. None of the biochemical indexes was affected by calcium supplementation, with the possible exception of bone alkaline phosphatase (−29% at 52 weeks; P = 0.015). These data demonstrate that lactation-associated changes in calcium and bone metabolism are physiological and are independent of dietary calcium supply in women with very low calcium intakes.

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Bone-Derived Serum Enzymes and Bone Density in Perimenopausal Caucasian Women

Annals of Clinical Biochemistry International Journal of Laboratory Medicine ◽

10.1177/000456329303000214 ◽

1993 ◽

Vol 30 (2) ◽

pp. 191-194 ◽

Cited By ~ 4

Author(s):

J D Johnston ◽

S Koneru ◽

T Kuwana ◽

S B Rosalki

Keyword(s):

Alkaline Phosphatase ◽

Acid Phosphatase ◽

Bone Density ◽

Femoral Neck ◽

Serum Levels ◽

Bone Alkaline Phosphatase ◽

Serum Enzymes ◽

Tartrate Resistant Acid Phosphatase ◽

Vertebral Bone ◽

Caucasian Women

Serum levels of bone-origin alkaline phosphatase and of tartrate-resistant acid phosphatase were measured in Caucasian women aged 41–69 years who had volunteered for bone densitometry. Bone alkaline phosphatase and tartrate-resistant acid phosphatase were inversely correlated with vertebral bone density and with femoral neck bone density. Bone alkaline phosphatase and acid phosphatase were also significantly correlated, consistent with the concept of ‘coupling’ between osteoblast and osteoclast activity.

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Putative Effects of Nutritive Polyphenols on Bone Metabolism In Vivo—Evidence from Human Studies

Nutrients ◽

10.3390/nu11040871 ◽

2019 ◽

Vol 11 (4) ◽

pp. 871 ◽

Cited By ~ 10

Author(s):

Katharina Austermann ◽

Natalie Baecker ◽

Peter Stehle ◽

Martina Heer

Keyword(s):

Bone Turnover ◽

Intervention Studies ◽

Inclusion Criteria ◽

Mineral Density ◽

Randomized Controlled ◽

Pubmed Database ◽

Habitual Diet ◽

Study Population ◽

Turnover Markers

For the prevention and treatment of bone loss related diseases, focus has been put on naturally derived substances such as polyphenols. Based on human intervention studies, this review gives an overview of the effects of dietary significant polyphenols (flavonoids, hydroxycinnamic acids, and stilbenes) on bone turnover. Literature research was conducted using PubMed database and articles published between 01/01/2008 and 31/12/2018 were included (last entry: 19/02/2019). Randomized controlled trials using oral polyphenol supplementation, either of isolated polyphenols or polyphenols-rich foods with healthy subjects or study populations with bone disorders were enclosed. Twenty articles fulfilled the inclusion criteria and the average study quality (mean Jadad score: 4.5) was above the pre-defined cut-off of 3.0. Evidence from these studies does not allow an explicit conclusion regarding the effects of dietary important polyphenols on bone mineral density and bone turnover markers. Differences in study population, habitual diet, lifestyle factors, applied polyphenols, used doses, and polyphenol bioavailability complicate the comparison of study outcomes.

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The Effects of Atmospheric Pressure Argon Plasma Treated Bovine Bone Substitute on Bone Regeneration

Coatings ◽

10.3390/coatings9120790 ◽

2019 ◽

Vol 9 (12) ◽

pp. 790

Author(s):

Jong-Ju Ahn ◽

Ji-Hyun Yoo ◽

Eun-Bin Bae ◽

Gyoo-Cheon Kim ◽

Jae Joon Hwang ◽

...

Keyword(s):

Bone Regeneration ◽

Atmospheric Pressure ◽

Bone Substitute ◽

Argon Plasma ◽

Atmospheric Pressure Plasma ◽

Regeneration Ability ◽

Bovine Bone ◽

Significant Difference

This study was undertaken to compare new bone formation between non-expired and expired bovine-derived xenogeneic bone substitute (expired, out-of-use period) and to evaluate the efficacy of argon (Ar)-based atmospheric pressure plasma (APP) treatment on expired bone substitute in rat calvarial defect. The groups were divided into (1) Non/Expired group (Using regular xenografts), (2) Expired group (Using expired xenografts), and (3) Ar/Expired group (Using Ar-based APP treated expired xenografts). Surface observation and cell experiments were performed in vitro. Twelve rats were used for in vivo experiment and the bony defects were created on the middle of the cranium. The bone substitute of each group was implanted into the defective site. After 4 weeks, all the rats were sacrificed, and the volumetric, histologic, and histometric analyses were performed. In the results of osteogenic differentiation and mineralization, Non/Expired and Ar/Expired groups were significantly higher than Expired group (p < 0.05). However, there was no significant difference between groups in the animal study (p > 0.05). Within the limitations of this study, the surface treatment of Ar-based APP has a potential effect on the surface modification of bone grafts. However, there was no significant difference in bone regeneration ability between groups in vivo; thus, studies on APP to enhance bone regeneration should be carried out in the future.

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Differential Patterns of Bone Turnover in Relation to Bone Pain and Disease Extent in Bone in Cancer Patients with Skeletal Metastases

Clinical Chemistry ◽

10.1093/clinchem/45.8.1240 ◽

1999 ◽

Vol 45 (8) ◽

pp. 1240-1247 ◽

Cited By ~ 80

Author(s):

Alfredo Berruti ◽

Luigi Dogliotti ◽

Gabriella Gorzegno ◽

Mirella Torta ◽

Marco Tampellini ◽

...

Keyword(s):

Alkaline Phosphatase ◽

Bone Turnover ◽

Urinary Excretion ◽

Bone Metastases ◽

Primary Tumor ◽

Bone Pain ◽

Bone Alkaline Phosphatase ◽

Skeletal Metastases ◽

Disease Extent ◽

Tumor Types

Abstract Background: The alteration of the bone microenvironment as a consequence of skeletal metastases is poorly understood. The aim of this study was to search for patterns of bone markers in relation to primary tumor type, bone pain, and number of sites involved in patients with bone metastases. Methods: We studied 323 patients with bone metastases from various primary malignancies. We sequentially measured the serum concentrations of bone alkaline phosphatase [by an electrophoretic technique (BALP)], carboxy-terminal telopeptide of type I collagen (ICTP), calcium (CaS), intact parathyroid hormone (PTH), and the fasting urinary excretion of calcium (Ca:Cr). Immunoradiometric serum bone alkaline phosphatase (I-BALP) and urinary excretion of deoxypyridinoline (DPYD) were also assessed in the 175 cases. Data were analyzed as a function of bone pain (assessed by a validated pain questionnaire), the number of radiographically confirmed sites of bone involvement, and the most frequent primary tumor types: breast cancer (BC; 124 patients), prostate cancer (PC; 90 patients), and non-small cell lung cancer (LC; 49 patients). Results: Serum BALP and I-BALP correlated with the number of radiologically identified blastic bone lesions. BALP and I-BALP were more frequently increased in PC (72% for both measurements) than in BC (50% and 60%, respectively) or LC (3% and 5%, respectively; P <0.001 for BALP and P = 0.001 for I-BALP). ICTP and DPYD values did not differ among PC, BC, and LC, but they did show a direct relationship with the disease extent in bone (P <0.001). CaS and Ca:Cr did not vary significantly according to the bone tumor burden. Bone pain directly correlated with ICTP (P <0.001), DPYD (P = 0.002), CaS (P <0.002), and Ca:Cr (P = 0.001), whereas the relationship was inverse for serum PTH (P = 0.002). When patients were stratified according to the primary tumor, ICTP correlated with the bone pain in all subsets (P <0.005, <0.005, and <0.001 for BC, PC, and LC, respectively), as did CaS and Ca:Cr in LC patients (P = 0.01 and 0.02, respectively) but not in PC and BC patients. Conclusions: The patterns of bone turnover markers differ among the primary tumor types. Both resorption and formation markers reflect the number of radiographically identified sites of bone metastases, whereas resorption markers and serum calcium but not formation markers correlate with bone pain.

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