scholarly journals Histone H2B ubiquitination mediated chromatin relaxation is essential for the induction of somatic cell reprogramming

2021 ◽  
Author(s):  
Liying Wang ◽  
Zhiliang Xu ◽  
Libin Wang ◽  
Chao Liu ◽  
Huafang Wei ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Cell Reprogramming ◽  
Histone H2b ◽  
Somatic Cell Reprogramming ◽  
Chromatin Relaxation

Chromatin architecture reorganization during somatic cell reprogramming

2021 ◽  
Vol 70 ◽  
pp. 104-113
Author(s):  
Kun Zhao ◽  
Mingzhu Wang ◽  
Shaorong Gao ◽  
Jiayu Chen
Keyword(s):  
Somatic Cell ◽  
Cell Reprogramming ◽  
Chromatin Architecture ◽  
Somatic Cell Reprogramming

scholarly journals miR-6539 is a novel mediator of somatic cell reprogramming that represses the translation of Dnmt3b

2017 ◽  
Vol 63 (4) ◽  
pp. 415-423 ◽  
Author(s):  
Fujia WU ◽  
Li TAO ◽  
Shuai GAO ◽  
Likun REN ◽  
Zhuqing WANG ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Cell Reprogramming ◽  
Somatic Cell Reprogramming

CCCTC-Binding Factor Transcriptionally Targets Wdr5 to Mediate Somatic Cell Reprogramming

2017 ◽  
Vol 26 (10) ◽  
pp. 743-750 ◽  
Author(s):  
Feng Wang ◽  
Jinghua Han ◽  
Li Wang ◽  
Ying Jing ◽  
Zhu Zhu ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Cell Reprogramming ◽  
Somatic Cell Reprogramming ◽  
Binding Factor

scholarly journals Sequenced Somatic Cell Reprogramming and Differentiation Inside Nested Hydrogel Droplets

2020 ◽  
Vol 4 (8) ◽  
pp. 2000071
Author(s):  
David W. Green ◽  
Jolanta A. Watson ◽  
Gregory S. Watson ◽  
Artemis Stamboulis
Keyword(s):  
Somatic Cell ◽  
Cell Reprogramming ◽  
Somatic Cell Reprogramming

scholarly journals Selective targeting of TET catalytic domain promotes somatic cell reprogramming

2020 ◽  
Vol 117 (7) ◽  
pp. 3621-3626 ◽  
Author(s):  
Anup Kumar Singh ◽  
Bo Zhao ◽  
Xiuhua Liu ◽  
Xin Wang ◽  
Hongzhi Li ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Catalytic Domain ◽  
Specific Binding ◽  
Screening Strategy ◽  
Cell Reprogramming ◽  
Promoter Regions ◽  
Somatic Cell Reprogramming ◽  
Enzymatic Function ◽  
Small Molecule Compound ◽  
Tet Enzymes

Ten-eleven translocation (TET) family enzymes (TET1, TET2, and TET3) oxidize 5-methylcytosine (5mC) and generate 5-hydroxymethylcytosine (5hmC) marks on the genome. Each TET protein also interacts with specific binding partners and partly plays their role independent of catalytic activity. Although the basic role of TET enzymes is well established now, the molecular mechanism and specific contribution of their catalytic and noncatalytic domains remain elusive. Here, by combining in silico and biochemical screening strategy, we have identified a small molecule compound, C35, as a first-in-class TET inhibitor that specifically blocks their catalytic activities. Using this inhibitor, we explored the enzymatic function of TET proteins during somatic cell reprogramming. Interestingly, we found that C35-mediated TET inactivation increased the efficiency of somatic cell programming without affecting TET complexes. Using high-throughput mRNA sequencing, we found that by targeting 5hmC repressive marks in the promoter regions, C35-mediated TET inhibition activates the transcription of the BMP-SMAD-ID signaling pathway, which may be responsible for promoting somatic cell reprogramming. These results suggest that C35 is an important tool for inducing somatic cell reprogramming, as well as for dissecting the other biological functions of TET enzymatic activities without affecting their other nonenzymatic roles.

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