Wilavidin* ‐ a novel member of the avidin family that forms unique biotin‐binding hexamers

FEBS Journal ◽  
2021 ◽  
Author(s):  
Orly Avraham ◽  
Edward A. Bayer ◽  
Oded Livnah
Keyword(s):  
Biotin Binding

Expression of various biotin-binding proteins in transgenic tobacco confers resistance to potato tuber moth, Phthorimaea operculella (Zeller) (fam. Gelechiidae)

2010 ◽  
Vol 19 (6) ◽  
pp. 1041-1051 ◽  
Author(s):  
Colleen Murray ◽  
Ngaire P. Markwick ◽  
Ryohei Kaji ◽  
Joanne Poulton ◽  
Harry Martin ◽  
...  
Keyword(s):  
Potato Tuber ◽  
Transgenic Tobacco ◽  
Binding Proteins ◽  
Phthorimaea Operculella ◽  
Potato Tuber Moth ◽  
Biotin Binding

scholarly journals A tomato cDNA encoding a biotin-binding protein

1987 ◽  
Vol 15 (9) ◽  
pp. 3928-3928 ◽  
Author(s):  
N. E. Hoffman ◽  
E. Pichersky ◽  
A. R. Cashmore
Keyword(s):  
Binding Protein ◽  
Biotin Binding

scholarly journals Biotin binding to avidin. Oligosaccharide side chain not required for ligand association

1987 ◽  
Vol 248 (1) ◽  
pp. 167-171 ◽  
Author(s):  
Y Hiller ◽  
J M Gershoni ◽  
E A Bayer ◽  
M Wilchek
Keyword(s):  
Concanavalin A ◽  
Sugar Content ◽  
Binding Activity ◽  
Side Chain ◽  
Affinity Column ◽  
Binding Properties ◽  
Commercial Preparation ◽  
Oligosaccharide Moiety ◽  
Biotin Binding

A commercially available, purified preparation of avidin was found to comprise two polypeptide bands (Mr 18,000 and Mr 15,500 respectively). Both bands bound biotin as assessed by biotin overlays of protein blots. The Mr 15,500 polypeptide was found to differ from the Mr 18,000 polypeptide only in its sugar content. When the commercial preparation was applied to a concanavalin A affinity column, the glycosylated forms were retarded as expected, and homotypic nonglycosylated avidin tetramers which failed to bind selectively to the column were collected in the effluent. The biotin-binding properties of the nonglycosylated avidin were equivalent to those obtained for the native (glycosylated) avidin molecule, indicating that the oligosaccharide moiety is not essential for the binding activity.

scholarly journals In situ observation of streptavidin-biotin binding on an immunoassay well surface using an atomic force microscope

FEBS Letters ◽  
1996 ◽  
Vol 390 (2) ◽  
pp. 161-164 ◽  
Author(s):  
S. Allen ◽  
J. Davies ◽  
A.C. Dawkes ◽  
M.C. Davies ◽  
J.C. Edwards ◽  
...  
Keyword(s):  
Atomic Force Microscope ◽  
In Situ Observation ◽  
Atomic Force ◽  
Biotin Binding

Electrochemical determination of avidin-biotin binding using an electroactive biotin derivative as a marker

1994 ◽  
Vol 33 (2) ◽  
pp. 205-207 ◽  
Author(s):  
Kazuharu Sugawara ◽  
Shunitz Tanaka ◽  
Hiroshi Nakamura
Keyword(s):  
Electrochemical Determination ◽  
Biotin Binding

scholarly journals Pre-hybridisation: An efficient way of suppressing endogenous biotin-binding activity inherent to biotin–streptavidin detection system

2014 ◽  
Vol 406 ◽  
pp. 143-147 ◽  
Author(s):  
Raju Ahmed ◽  
Emma Spikings ◽  
Shaobo Zhou ◽  
Andrew Thompsett ◽  
Tiantian Zhang
Keyword(s):  
Detection System ◽  
Binding Activity ◽  
Biotin Binding ◽  
Endogenous Biotin

Optofluidic twin-core hollow fiber interferometer for label-free sensing of the streptavidin-biotin binding

2018 ◽  
Vol 277 ◽  
pp. 353-359 ◽  
Author(s):  
Xinghua Yang ◽  
Wenting Yu ◽  
Zhihai Liu ◽  
Jun Yang ◽  
Yu Zhang ◽  
...  
Keyword(s):  
Hollow Fiber ◽  
Label Free ◽  
Biotin Binding
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