Mosaic translation hypothesis: chimeric polypeptides produced via multiple ribosomal frameshifting as a basis for adaptability

FEBS Journal ◽  
2021 ◽  
Author(s):  
Umut Çakır ◽  
Noujoud Gabed ◽  
Marie Brunet ◽  
Xavier Roucou ◽  
Igor Kryvoruchko
Keyword(s):  
Ribosomal Frameshifting

scholarly journals Modulation of Viral Programmed Ribosomal Frameshifting and Stop Codon Readthrough by the Host Restriction Factor Shiftless

Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1230
Author(s):  
Sawsan Napthine ◽  
Chris H. Hill ◽  
Holly C. M. Nugent ◽  
Ian Brierley
Keyword(s):  
Rna Binding ◽  
Mutational Analysis ◽  
Stop Codon ◽  
Leukemia Virus ◽  
Size Exclusion ◽  
Human Immunodeficiency Virus Replication ◽  
Mobility Shift ◽  
Ribosomal Frameshifting ◽  
Stop Codon Readthrough

The product of the interferon-stimulated gene C19orf66, Shiftless (SHFL), restricts human immunodeficiency virus replication through downregulation of the efficiency of the viral gag/pol frameshifting signal. In this study, we demonstrate that bacterially expressed, purified SHFL can decrease the efficiency of programmed ribosomal frameshifting in vitro at a variety of sites, including the RNA pseudoknot-dependent signals of the coronaviruses IBV, SARS-CoV and SARS-CoV-2, and the protein-dependent stimulators of the cardioviruses EMCV and TMEV. SHFL also reduced the efficiency of stop-codon readthrough at the murine leukemia virus gag/pol signal. Using size-exclusion chromatography, we confirm the binding of the purified protein to mammalian ribosomes in vitro. Finally, through electrophoretic mobility shift assays and mutational analysis, we show that expressed SHFL has strong RNA binding activity that is necessary for full activity in the inhibition of frameshifting, but shows no clear specificity for stimulatory RNA structures.

scholarly journals Unfolding Intermediate of mRNA Pseudoknot Correlates with Ribosomal Frameshifting

2018 ◽  
Vol 114 (3) ◽  
pp. 592a-593a
Author(s):  
Kai-Chun Chang ◽  
Po-Szu Hsieh ◽  
An-Yi Lee ◽  
Emmanuel Salawu ◽  
You-Hsin Lin ◽  
...  
Keyword(s):  
Ribosomal Frameshifting

scholarly journals Mutation of a highly conserved base in the yeast mitochondrial 21S rRNA restricts ribosomal frameshifting

1995 ◽  
Vol 248 (2) ◽  
pp. 207-216 ◽  
Author(s):  
Brigitte Weiss-Brummer ◽  
Alfred Zollner ◽  
Albert Haid ◽  
Shahla Thomnson
Keyword(s):  
Ribosomal Frameshifting

Resolution-exchanged structural modeling and simulations jointly unravel that subunit rolling underlies the mechanism of programmed ribosomal frameshifting

2018 ◽  
Vol 35 (6) ◽  
pp. 945-952 ◽  
Author(s):  
Kai-Chun Chang ◽  
Emmanuel Oluwatobi Salawu ◽  
Yuan-Yu Chang ◽  
Jin-Der Wen ◽  
Lee-Wei Yang
Keyword(s):  
Structural Modeling ◽  
Modeling And Simulations ◽  
Ribosomal Frameshifting

Expression of the Rous sarcoma virus pol gene by ribosomal frameshifting

Science ◽  
1985 ◽  
Vol 230 (4731) ◽  
pp. 1237-1242 ◽  
Author(s):  
T Jacks ◽  
H. Varmus
Keyword(s):  
Rous Sarcoma Virus ◽  
Ribosomal Frameshifting ◽  
Rous Sarcoma ◽  
Sarcoma Virus

scholarly journals Competing pathways control host resistance to virus via tRNA modification and programmed ribosomal frameshifting

2012 ◽  
Vol 8 (1) ◽  
pp. 567 ◽  
Author(s):  
Nathaniel D Maynard ◽  
Derek N Macklin ◽  
Karla Kirkegaard ◽  
Markus W Covert
Keyword(s):  
Host Resistance ◽  
Trna Modification ◽  
Ribosomal Frameshifting ◽  
Competing Pathways

scholarly journals Accounting for Programmed Ribosomal Frameshifting in the Computation of Codon Usage Bias Indices

2018 ◽  
Vol 8 (10) ◽  
pp. 3173-3183 ◽  
Author(s):  
Victor Garcia ◽  
Stefan Zoller ◽  
Maria Anisimova
Keyword(s):  
Codon Usage ◽  
Codon Usage Bias ◽  
Ribosomal Frameshifting
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