Thy.1lowCD3−Cells Sorted from Nylon Wool-Passed Bone Marrow Cells Can Augment the H-2 Identical but Not Non-Identical Cytotoxic T Lymphocyte Precursors in Mixed Lymphocyte Cultures

In SCID (severe combined immunodeficient) mice, proper assembly of immunoglobulin and T cell receptor (TCR) genes is blocked by defective V(D)J recombination so that B and T lymphocyte differentiation is arrested at an early precursor stage. Treating the mice with gamma irradiation rescues V(D)J rearrangement at multiple TCR loci, promotes limited thymocyte differentiation, and induces thymic lymphomas. These effects are not observed in the B cell lineage. Current models postulate that irradiation affects intrathymic T cell precursors. Surprisingly, we found that transfer of irradiated SCID bone marrow cells to unirradiated host animals rescues both TCR rearrangements and thymocyte differentiation. These data indicate that irradiation affects precursor cells at an earlier stage of differentiation than was previously thought and suggest new models for the mechanism of irradiation rescue.

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A myelopeptide from unstimulated bone marrow cells with immunomodulatory actiivity in lymphocyte cultures from healthy donors and patients with hypogammaglobulinemia and active systemic lupus erythematosus

International Journal of Immunopharmacology ◽

10.1016/0192-0561(88)90146-4 ◽

1988 ◽

Vol 10 (1) ◽

pp. 23-28 ◽

Cited By ~ 4

Author(s):

S. Jahn ◽

H.-D. Volk ◽

R. Grunow ◽

S.T. Kiessig ◽

F. Hiepe ◽

...

Keyword(s):

Systemic Lupus Erythematosus ◽

Bone Marrow ◽

Lupus Erythematosus ◽

Bone Marrow Cells ◽

Active Systemic Lupus Erythematosus ◽

Systemic Lupus ◽

Healthy Donors ◽

Lymphocyte Cultures ◽

Marrow Cells

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T lymphocyte differentiation in vitro from adult human prethymic CD34+ bone marrow cells.

Journal of Experimental Medicine ◽

10.1084/jem.177.6.1531 ◽

1993 ◽

Vol 177 (6) ◽

pp. 1531-1539 ◽

Cited By ~ 51

Author(s):

G E Tjønnfjord ◽

O P Veiby ◽

R Steen ◽

T Egeland

Keyword(s):

Bone Marrow ◽

T Lymphocyte ◽

Bone Marrow Cells ◽

Cell Receptor ◽

Thymic Tissue ◽

Adult Human ◽

Human Cd34 ◽

Cd34 Cells ◽

Marrow Cells

Pluripotent lymphohematopoietic stem cells are probably confined to bone marrow cells expressing CD34 surface molecules. To investigate the capacity of adult human CD34+ bone marrow cells to differentiate along the T lymphoid lineage, we plated purified CD34+ cells from healthy adults in liquid culture on adherent thymic stromal cells prepared from HLA- or blood group-mismatched postnatal thymic tissue. We show that purified CD34+CD3-CD4-CD8- bone marrow cells contained progenitors with the ability to differentiate into CD4+ and CD8+ T lymphocytes expressing surface (s)CD3 and T cell receptor alpha/beta in vitro. These progenitors were found in the CD34+CD2+sCD3-CD4-CD8-, CD34+CD7+sCD3-CD4-CD8-, and CD34+CD2+CD7+sCD3-CD4-CD8-, as well as in the CD34+CD2-sCD3-CD4-CD8-, CD34+CD7-sCD3-CD4-CD8-, and CD34+CD2-CD7-sCD3-CD4-CD8- subsets, indicating that T lymphocyte progenitors sensitive to signals mediated by thymic stroma in vitro are not restricted to CD34+ cells already coexpressing early T lymphocyte-associated markers. Finally, we show that T lymphopoiesis was enhanced by c-kit ligand.

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Quantitative studies on the precursors of cytotoxic lymphocytes. VI. Second signal requirements of specifically activated precursors isolated 12 h after stimulation.

Journal of Experimental Medicine ◽

10.1084/jem.151.1.12 ◽

1980 ◽

Vol 151 (1) ◽

pp. 12-19 ◽

Cited By ~ 37

Author(s):

M E Lalande ◽

M J McCutcheon ◽

R G Miller

Keyword(s):

Cytotoxic T Lymphocytes ◽

T Lymphocyte ◽

Mixed Lymphocyte Reaction ◽

Cytotoxic T Lymphocyte ◽

Cytotoxic Lymphocytes ◽

Spleen Cells ◽

Quantitative Studies ◽

Partial Activation ◽

Lymphocyte Cultures ◽

Mixed Lymphocyte Cultures

It is shown that, in a mixed lymphocyte reaction, the production of cytotoxic T lymphocytes (CL) from cytotoxic T lymphocyte precursors (CLP) requires two signals which are separated time. Using a flow cytometer-cell sorter and a vital, fluorescent DNA stain, Hoechst 3342, CLP specific for the stimulator cells can be separated from other CLP and from stimulator cells 12 h after initiation of mixed lymphocyte cultures. These CLP are in a state of partial activation and can produce CL in the absence of stimulator cells if a second signal in the form of a concanavalin A-induced spleen cell supernate factor is added. Specific CL are also generated when the partially activated CLP are cultured with both nude spleen cells and stimulator cells. In this case it appears that an interaction between the stimulator cells and the nude spleen cells leads to production of the second signal.

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