scholarly journals Immunohistochemical diagnosis of the cagA-gene genotype of Helicobacter pylori with anti-East Asian CagA-specific antibody

2007 ◽  
Vol 98 (4) ◽  
pp. 521-528 ◽  
Author(s):  
Tomohisa Uchida ◽  
Ryoko Kanada ◽  
Yoshiyuki Tsukamoto ◽  
Naoki Hijiya ◽  
Keiko Matsuura ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Specific Antibody ◽  
East Asian ◽  
Caga Gene ◽  
Immunohistochemical Diagnosis

scholarly journals Genotyping of the cagA gene of Helicobacter pylori on immunohistochemistry with East Asian CagA-specific antibody

2008 ◽  
Vol 58 (4) ◽  
pp. 218-225 ◽  
Author(s):  
Ryoko Kanada ◽  
Tomohisa Uchida ◽  
Yoshiyuki Tsukamoto ◽  
Lam Tung Nguyen ◽  
Naoki Hijiya ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Specific Antibody ◽  
East Asian ◽  
Caga Gene

Prevalence of East Asian (East CagA) and Western variants of the cagA (Western cagA) gene of Helicobacter pylori in Yakutia

2021 ◽  
pp. 63-67
Author(s):  
N.N. Gotovsev ◽  
N.A. Barashkov ◽  
V.G. Pshennikova ◽  
M.V. Pak ◽  
K.C. Loskutova ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
East Asian ◽  
Caga Gene

scholarly journals Prevalence, diversity and disease association of Helicobacter pylori in dyspeptic patients from Pakistan

2013 ◽  
Vol 7 (03) ◽  
pp. 220-228 ◽  
Author(s):  
Adnan Khan ◽  
Amber Farooqui ◽  
Yasir Raza ◽  
Faisal Rasheed ◽  
Hamid Manzoor ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
Disease Burden ◽  
Detection Rate ◽  
East Asian ◽  
Disease Association ◽  
Gastric Biopsy ◽  
Caga Gene ◽  
H Pylori ◽  
Type Strains

Introduction: The etiological association of Helicobacter pylori with gastric ulcer (GU), gastric cancer (GC), and duodenal ulcer (DU) is well-known. Understanding the epidemiology of H. pylori facilitates the estimation of disease burden in a certain population. This study presents the diversity of H. pylori genotypes and their association with different clinical outcomes among dyspeptic patients in Pakistan over a period of four years. Methodology: Gastric biopsy samples from a total of 450 dyspeptic individualswere subjected to PCR, genotypingand histology. Results: A total of 201 (45%) cases were found positive for H. pylori. The detection rate was high in GU (91%), DU (86%) and GC (83%) cases compared with those cases who had intact gastric mucosa (18%). Histology revealed the presence of infection in 68% of cases of mild/chronic nonspecific gastritis with others belonging to the GU sequel. cagA gene carriage was observed in 104 (51%) cases or mostly from DU, GU and GC groups, of which 97 were Western type strains while 3 were East-Asian type strains that are rarely observed in South Asia. vacA allelic variant s1am1 was most commonly observed, followed by s1am2, and s1bm1, with direct correlation in diseased cases (gastritis, GU, DU and GC). Prevalent genotypic combinations were s1am1/cagA- in gastritis and s1am1/cagA+ in DU, GU, and GC. Conclusions: Our study indicates the predominant circulation of Western type cagA and vacAs1am1 type H. pylori strains in Pakistan.

Diversity of Helicobacter pylori CagA gene and its clinical significance

2001 ◽  
Vol 120 (5) ◽  
pp. A56-A56
Author(s):  
T AZUMA ◽  
Y ITO ◽  
M DOJO
Keyword(s):  
Helicobacter Pylori ◽  
Clinical Significance ◽  
Caga Gene

Endoscopic Biopsy Pathology of Helicobacter pylori Gastritis

1999 ◽  
Vol 123 (9) ◽  
pp. 778-781 ◽  
Author(s):  
Maher Toulaymat ◽  
Sharon Marconi ◽  
Jane Garb ◽  
Christopher Otis ◽  
Shirin Nash
Keyword(s):  
Helicobacter Pylori ◽  
Specific Antibody ◽  
Cost Effective ◽  
Endoscopic Biopsy ◽  
Immunohistochemical Method ◽  
Labor Costs ◽  
Chronic Active Gastritis ◽  
Cost Effective Method ◽  
H Pylori ◽  
Helicobacter Pylori Gastritis

Abstract Objectives.—To describe the endoscopic biopsy pathology of Helicobacter pylori gastritis, compare bacterial detection by immunohistochemistry using a specific antibody with the Genta stain, and to compare the relative costs of the 2 techniques. Design.—One hundred cases of gastritis identified as positive for H pylori by Genta stain and 100 cases considered negative by the same technique were stained using an anti-H pylori–specific polyclonal antibody. Laboratory reagent and labor costs for the 2 methods were compared. Results.—Chronic active gastritis with lymphoid follicles was significantly associated with H pylori infection (P < .0001). The immunohistochemical method had a sensitivity of 97% and a specificity of 98% compared with the Genta stain, with strong agreement for grading density of organisms (κ = 0.85; P < .001). Reagent costs were similar for both methods, but immunohistochemistry using an autoimmunostainer required less dedicated technical time and hence was less expensive than the Genta stain. Conclusions.—Immunohistochemistry using a specific antibody is an accurate and cost-effective method for H pylori detection in gastric biopsies.

Characterization of East-Asian Helicobacter pylori encoding Western EPIYA-ABC CagA

2021 ◽  
Author(s):  
Kavinda Tissera ◽  
Myeong-A Kim ◽  
Jing Lai ◽  
Sacheera Angulmaduwa ◽  
Aeryun Kim ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
East Asian

scholarly journals Western-Type Helicobacter pylori CagA are the Most Frequent Type in Mongolian Patients

Cancers ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 725 ◽  
Author(s):  
Tegshee Tserentogtokh ◽  
Boldbaatar Gantuya ◽  
Phawinee Subsomwong ◽  
Khasag Oyuntsetseg ◽  
Dashdorj Bolor ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
Cancer Patients ◽  
East Asian ◽  
Virulent Strains ◽  
Virulent Type ◽  
High Incidence ◽  
Caga Status ◽  
H Pylori ◽  
Gastric Cancer Patients

Helicobacter pylori infection possessing East-Asian-type CagA is associated with carcinogenesis. Mongolia has the highest mortality rate from gastric cancer. Therefore, we evaluated the CagA status in the Mongolian population. High risk and gastric cancer patients were determined using endoscopy and histological examination. H. pylori strains were isolated from different locations in Mongolia. The CagA subtypes (East-Asian-type or Western-type, based on sequencing of Glu-Pro-Ile-Tyr-Ala (EPIYA) segments) and vacA genotypes (s and m regions) were determined using PCR-based sequencing and PCR, respectively. In total, 368 patients were examined (341 gastritis, 10 peptic ulcer, and 17 gastric cancer). Sixty-two (16.8%) strains were cagA-negative and 306 (83.1%) were cagA-positive (293 Western-type, 12 East-Asian-type, and one hybrid type). All cagA-negative strains were isolated from gastritis patients. In the gastritis group, 78.6% (268/341) had Western-type CagA, 2.9% (10/341) had East-Asian-type, and 18.2% (61/341) were cagA-negative. However, all H. pylori from gastric cancer patients possessed Western-type CagA. Histological analyses showed that East-Asian-type CagA was the most virulent strains, followed by Western-type and cagA-negative strains. This finding agreed with the current consensus. CagA-positive strains were the most virulent type. However, the fact that different CagA types can explain the high incidence of gastric cancer might be inapplicable in Mongolia.

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