The Minor Components of Normal Human Adult Haemoglobin

1960 ◽  
Vol 6 (2) ◽  
pp. 191-197 ◽  
Author(s):  
W. B. Gratzer ◽  
G. H. Beaven
1963 ◽  
Vol 9 (2) ◽  
pp. 135-145 ◽  
Author(s):  
Masumi Nakamichi ◽  
Samuel Raymond

Abstract Details of a technic for analysis of hemoglobin mixtures using acrylamide-gel electrophoresis are presented. The method offers increased speed and reproducibility. Measurements on the unstained gel are made at 525 and at 420 m µ in a procedure affording increased precision of measurements for minor components. The gel is then stained with amidoblack to detect and measure nonhemoglobin components. The major component of normal human adult hemoglobin is separated into two components in this medium. The two components (A1, A3) appear in varying relative amounts depending on the source of the specimen. The normal minor component A2 also appears. After staining the pattern with a protein dye such as amidoblack 10B, additional components appear in the electrophoresis patterns of hemolysates from carefully washed red cells. These components have a constant migration ratio referred to hemoglobin A1 and appear to be nonheme-containing intraerythrocytic proteins.


1978 ◽  
Vol 253 (7) ◽  
pp. 2327-2332 ◽  
Author(s):  
M.J. McDonald ◽  
R. Shapiro ◽  
M. Bleichman ◽  
J. Solway ◽  
H.F. Bunn

2010 ◽  
Vol 66 (11) ◽  
pp. 1144-1152 ◽  
Author(s):  
Andrey Kovalevsky ◽  
Toshiyuki Chatake ◽  
Naoya Shibayama ◽  
Sam-Yong Park ◽  
Takuya Ishikawa ◽  
...  

1989 ◽  
Vol 183 (2) ◽  
pp. 419-423 ◽  
Author(s):  
Kazuyuki HIRANO ◽  
Kazutomi KUSANO ◽  
Yoshikazu MATSUMOTO ◽  
Torgny STIGBRAND ◽  
Shiro IINO ◽  
...  

1990 ◽  
Vol 27 (1) ◽  
pp. 26-35 ◽  
Author(s):  
David P. Kuehn ◽  
Joel C. Kahane

Blood ◽  
1947 ◽  
Vol 2 (Special_Issue_Number_1) ◽  
pp. 54-59 ◽  
Author(s):  
STUART L. VAUGHAN ◽  
FRANCES BROCKMYRE

Abstract A study of aspirated sternal bone marrow of 50 normal human adult volunteer subjects has been presented. Samples of exactly 3 cc. each were collected in a uniform manner. The technic of performing total nucleated cell counts, differential counts, reticulocyte counts, and megakaryocyte counts has been described and the results given. These results have been subjected to statistical analysis and compared with those of other workers.


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