Development of highly sensitive and specific in vitro renal solute carrier (SLC) uptake cell models using normal human adult renal proximal tubule epithelial cells for drug transporter interaction studies

2018 ◽  
Vol 295 ◽  
pp. S114-S115
Author(s):  
R. Menth ◽  
C. Zou ◽  
L. Romero ◽  
E. Turner ◽  
K. Huang ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Proximal Tubule ◽  
Renal Proximal Tubule ◽  
Drug Transporter ◽  
Cell Models ◽  
Human Adult ◽  
Highly Sensitive ◽  
Normal Human ◽  
Solute Carrier

Development of SLC transporters kidney cell models using hTERT-immortalized renal proximal tubule epithelial cells (RPTEC/TERT1)

2017 ◽  
Vol 280 ◽  
pp. S139
Author(s):  
Sara Gualdoni ◽  
Chaozhong Zou ◽  
Russell McDaniel ◽  
Christopher Annesi ◽  
Elisabeth Turner
Keyword(s):  
Epithelial Cells ◽  
Proximal Tubule ◽  
Kidney Cell ◽  
Renal Proximal Tubule ◽  
Cell Models ◽  
Slc Transporters

scholarly journals Experimental Cell Line Models for Nephrotoxicity Screening

2021 ◽  
Vol 11 (3) ◽  
pp. 160-166
Author(s):  
I. A. Mazerkina ◽  
V. A. Evteev ◽  
A. B. Prokofiev ◽  
O. V. Muslimova ◽  
E. Yu. Demchenkova
Keyword(s):  
Epithelial Cell ◽  
Proximal Tubule ◽  
Cell Lines ◽  
Test Methods ◽  
Renal Proximal Tubule ◽  
Cell Models ◽  
Proximal Tubule Epithelial Cell ◽  
Epithelial Cell Lines

The aim of the study was to review literature data on cell models for experimental assessment of drug nephrotoxicity in vitro. Because of nephrotoxicity, 2% of new investigational medicinal products are discarded at the stage of preclinical in vivo studies in laboratory animals, and 19%—after phase 3 clinical trials. Prediction of toxicity in cell models could make drug development more cost-effective and help to reduce/avoid animal testing. At present, there are no official international guidelines for assessment of nephrotoxicity in vitro, but there is a lot of research underway. The main toxicity target in kidneys is renal proximal tubule epithelial cells, therefore the main research is focused on the development of renal proximal tubule epithelial cell lines with stable functional characteristics. Another important aspect in nephrotoxicity modeling is the choice of relevant test methods and end points which would reflect potential toxicity mechanisms. The paper reviews existing human renal proximal tubule epithelial cell lines and current test methods for assessing cytotoxicity. Promising areas for future development of cell models for nephrotoxicity assessment— are optimisation and standardisation of in vitro systems that would help to make preclinical predictions of drug nephrotoxicity in vivo.  

scholarly journals Urine-Derived Epithelial Cells as Models for Genetic Kidney Diseases

Cells ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1413
Author(s):  
Tjessa Bondue ◽  
Fanny O. Arcolino ◽  
Koenraad R. P. Veys ◽  
Oyindamola C. Adebayo ◽  
Elena Levtchenko ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Kidney Diseases ◽  
Cell Types ◽  
Cell Models ◽  
Tubular Epithelial Cells ◽  
Disease Mechanisms ◽  
Proximal Tubular Epithelial Cells ◽  
Proximal Tubular ◽  
Disease Cell

Epithelial cells exfoliated in human urine can include cells anywhere from the urinary tract and kidneys; however, podocytes and proximal tubular epithelial cells (PTECs) are by far the most relevant cell types for the study of genetic kidney diseases. When maintained in vitro, they have been proven extremely valuable for discovering disease mechanisms and for the development of new therapies. Furthermore, cultured patient cells can individually represent their human sources and their specific variants for personalized medicine studies, which are recently gaining much interest. In this review, we summarize the methodology for establishing human podocyte and PTEC cell lines from urine and highlight their importance as kidney disease cell models. We explore the well-established and recent techniques of cell isolation, quantification, immortalization and characterization, and we describe their current and future applications.

QS394. The Genomic Analysis of the Heterogeneity Hypoxia Responses in Renal Proximal Tubule Epithelial Cells After Knock-Down of HIF-1α and HIF-2α

2008 ◽  
Vol 144 (2) ◽  
pp. 424
Author(s):  
Zhen Wang ◽  
Edwin H. Rodriguez ◽  
Michael T. Longaker ◽  
Patrick O. Brown ◽  
Jen-Tsan A. Chi ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Proximal Tubule ◽  
Genomic Analysis ◽  
Renal Proximal Tubule ◽  
Knock Down
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