Aggregation of thyroid autoantibodies in first-degree relatives of patients with autoimmune thyroid disease is mainly due to genes: a twin study

2004 ◽  
Vol 60 (3) ◽  
pp. 329-334 ◽  
Author(s):  
Thomas Heiberg Brix ◽  
Pia Skov Hansen ◽  
Kirsten Ohm Kyvik ◽  
Laszlo Hegedus
Keyword(s):  
Thyroid Disease ◽  
Twin Study ◽  
Autoimmune Thyroid Disease ◽  
Thyroid Autoantibodies ◽  
Autoimmune Thyroid ◽  
First Degree Relatives

scholarly journals Autoimmune Thyroid Disease Correlates to Islet Autoimmunity on Zinc Transporter 8 Autoantibody: A Cross-Section Study

2020 ◽  
Author(s):  
Yun Cai ◽  
Jieni Yan ◽  
Yong Gu ◽  
Heng Chen ◽  
Qingfang Hu ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Thyroid Disease ◽  
Autoimmune Thyroid Disease ◽  
Islet Autoantibodies ◽  
Islet Autoimmunity ◽  
Thyroid Autoantibodies ◽  
Healthy Controls ◽  
Autoimmune Thyroid ◽  
Relative Value

Abstract Background The most common coexisting organ-specific autoimmune disease in patients with Type 1 diabetes mellitus (T1DM) is autoimmune thyroid disease (AITD). Many studies have showed prevalence rate of thyroid autoantibodies range from 3.7-35% in T1DM patients, while some of them suggested the associations between thyroid autoantibodies and islet autoantibodies. However, little work has been done about the anti-islet autoimmune status in patients with autoimmune thyroid disease (AITD), and so far there have been no clinical report based on large population about zinc transporter 8 autoantibody (ZnT8A) in patients with AITD. We aimed to explore the presence of islet autoantibodies, ZnT8A, glutamic acid decarboxylase autoantibodies (GADA) and tyrosine phosphatase autoantibodies (IA-2A) compared with thyroid autoantibodies, thyroid peroxidase autoantibodies (TPOAb) and thyroglobulin autoantibodies (TGAb) and thyrotropin receptor autoantibodies (TRAb) in AITD patients. Methods In total 740 AITD patients, 108 type 1 diabetes mellitus (T1DM) patients with AITD, 172 non-autoimmune thyroid disease (nAITD) patients and 115 healthy controls were recruited in the cross-sectional study. Islet autoantibodies, ZnT8A, GADA, IA-2A and thyroid autoantibodies, TPOAb, TGAb, TRAb were detected with Radioimmunoassay and Chemiluminescence. Islet autoantibody relative value was established to compare the distribution of the three islet autoantibodies. Results The prevalence of ZnT8A and GADA in AITD group was significantly higher than that in healthy controls (ZnT8A: 15.00% vs 1.74%, GADA: 7.97% vs 0.87%, both P<0.05). Similarly, the prevalence of IA-2A in AITD group was higher than that in healthy controls (4.19% vs 0%, P<0.05). However, any islet autoantibodies positive rate in AITD group was significantly lower than that in T1DM with AITD group. Analysis of multivariable linear regression suggested that ZnT8A relative value was positively related with GADA relative value (β=0.352, P<0.01) and TPOAb titer (β=0.002, P<0.01), and GADA relative value was also positively related with ZnT8A relative value (β=0.183, P<0.01). Conclusions An increased prevalence of ZnT8A as well as a relatively high prevalence of islet autoimmunity was found in AITD patients, indicating that there is a potential link between thyroid autoimmunity and islet autoimmunity. Trial registration Retrospectively registered.

Familial Aggregation of Autoimmune Thyroiditis in First-Degree Relatives of Patients with Juvenile Autoimmune Thyroid Disease

Thyroid ◽  
2003 ◽  
Vol 13 (3) ◽  
pp. 297-300 ◽  
Author(s):  
R.K. Marwaha ◽  
Saurav Sen ◽  
Nikhil Tandon ◽  
M. Sahoo ◽  
R.P. Walia ◽  
...  
Keyword(s):  
Autoimmune Thyroiditis ◽  
Thyroid Disease ◽  
Autoimmune Thyroid Disease ◽  
Familial Aggregation ◽  
Autoimmune Thyroid ◽  
First Degree Relatives

Further Evidence for a Strong Genetic Influence on the Development of Autoimmune Thyroid Disease: The California Twin Study

Thyroid ◽  
2002 ◽  
Vol 12 (8) ◽  
pp. 647-653 ◽  
Author(s):  
Daniel A. Ringold ◽  
John T. Nicoloff ◽  
Matthew Kesler ◽  
Heather Davis ◽  
Ann Hamilton ◽  
...  
Keyword(s):  
Thyroid Disease ◽  
Twin Study ◽  
Autoimmune Thyroid Disease ◽  
Genetic Influence ◽  
Autoimmune Thyroid

scholarly journals Biological variability of thyroid autoantibodies (anti-TPO and anti-Tg) in clinically and biochemically stable patients with autoimmune thyroid disease

2002 ◽  
Vol 16 (1) ◽  
pp. 37-39 ◽  
Author(s):  
Concepción González ◽  
Monserrat Hernando ◽  
Fernando Cava ◽  
Eva Herrero ◽  
Luis Carlos García-Díez ◽  
...  
Keyword(s):  
Thyroid Disease ◽  
Autoimmune Thyroid Disease ◽  
Thyroid Autoantibodies ◽  
Biological Variability ◽  
Autoimmune Thyroid

Serum BAFF and thyroid autoantibodies in autoimmune thyroid disease

2016 ◽  
Vol 462 ◽  
pp. 96-102 ◽  
Author(s):  
Jiunn-Diann Lin ◽  
Yuan-Hung Wang ◽  
Wen-Fang Fang ◽  
Chia-Jung Hsiao ◽  
Amarzaya Chagnaadorj ◽  
...  
Keyword(s):  
Thyroid Disease ◽  
Autoimmune Thyroid Disease ◽  
Thyroid Autoantibodies ◽  
Autoimmune Thyroid

Harmonization in autoimmune thyroid disease diagnostics

2018 ◽  
Vol 56 (10) ◽  
pp. 1778-1782 ◽  
Author(s):  
Renato Tozzoli ◽  
Nicola Bizzaro
Keyword(s):  
Thyroid Disease ◽  
Autoimmune Thyroid Disease ◽  
Thyroid Autoantibodies ◽  
Thyroid Autoantibody ◽  
Autoimmune Thyroid ◽  
Disease Diagnostics ◽  
Reference Limits ◽  
Measurement Units ◽  
Total Testing Process ◽  
Definition Of

Abstract In this review we analyze all aspects of total testing process regarding the measurement of antithyroid peroxidase, antithyroglobulin and antithyrotropin receptor autoantibodies. The main critical points related to the preanalytical, analytical and postanalytical steps of autoimmune thyroid disease diagnostics are considered, focusing on harmonization of autoimmune thyroid tests request, retesting intervals, terminology of thyroid autoantibodies, measurement units and definition of reference limits. Harmonization in thyroid autoantibody testing is a relevant example of feasible harmonization in autoimmunology.

scholarly journals Autoimmune thyroid disease correlates to islet autoimmunity on zinc transporter 8 autoantibody

2021 ◽  
Author(s):  
Yun Cai ◽  
Jieni Yan ◽  
Yong Gu ◽  
Heng Chen ◽  
Yang Chen ◽  
...  
Keyword(s):  
Thyroid Disease ◽  
Autoimmune Thyroid Disease ◽  
Large Population ◽  
Zinc Transporter ◽  
Islet Autoantibodies ◽  
Islet Autoimmunity ◽  
Thyroid Peroxidase ◽  
Thyroid Autoantibodies ◽  
Autoimmune Thyroid ◽  
Zinc Transporter 8

Objective: The most common coexisting organ-specific autoimmune disease in patients with type 1 diabetes mellitus (T1DM) is autoimmune thyroid disease (AITD). However, there have been little clinical reports based on large population about the prevalence of zinc transporter 8 autoantibody (ZnT8A) and other islet autoantibodies in AITD patients. We aimed to explore the presence of islet autoantibodies, ZnT8A, glutamic acid decarboxylase autoantibodies (GADA) and insulinoma-associated antigen 2 autoantibodies (IA-2A) compared with thyroid autoantibodies, thyroid peroxidase autoantibodies (TPOAb) and thyroglobulin autoantibodies (TGAb) and thyrotropin receptor autoantibodies (TRAb) in patients with Graves’ disease (GD), Hashimoto’s thyroiditis (HT) and T1DM patients with AITD. Methods: Totally, 389 patients with GD, 334 patients with HT, 108 T1DM patients with AITD and 115 healthy controls (HC) were recruited in the study. Islet autoantibodies (ZnT8A, GADA and IA-2A) were detected by radioligand binding assay. Thyroid autoantibodies, TPOAb and TGAb were detected by chemiluminescence assay, and TRAb was detected by radioimmunoassay. Results: The prevalence of ZnT8A, GADA and IA-2A was higher in GD and HT patients than that of HC (ZnT8A: GD 8.48%, HT 10.8% vs HC 1.74%; GADA: GD 7.46%, HT 7.74% vs HC 0.870%; IA-2A: GD 4.88%, HT 3.59% vs HC 0%; All P<0.05); but lower than that of T1DM subjects with AITD (ZnT8A: 42.6%; IA-2A: 44.4%; GADA: 74.1%; all P<0.0001). Conclusions: An increased prevalence of ZnT8A as well as GADA and IA-2A was found in both GD and HT patients, indicating that there is a potential link between thyroid autoimmunity and islet autoimmunity.

Molecular cloning of antigens to thyroid autoantibodies using the expression vector lambda gt 11

1987 ◽  
Vol 116 (1_Suppl) ◽  
pp. S139-S145 ◽  
Author(s):  
Basil Rapoport ◽  
Hideshi Hirayu ◽  
Pui Seto ◽  
Ronald P. Magnusson
Keyword(s):  
Molecular Cloning ◽  
Cdna Library ◽  
Thyroid Disease ◽  
Autoimmune Thyroid Disease ◽  
Host Protein ◽  
Thyroid Autoantibodies ◽  
Thyroid Cells ◽  
High Background ◽  
Autoimmune Thyroid ◽  
Rat Thyroid

Abstract. The molecular cloning of certain antigens to thyroid autoantibodies present in patients with autoimmune thyroid disease would be of great value in further understanding the pathogenesis of these diseases, and in devising new approaches for their treatment. The ideal method for molecular cloning is to first obtain a partial amino acid sequence of a purified protein and to construct an oligonucleotide probe for screening an appropriate cDNA library. Unfortunately in the case if thyroid autoimmunity, important antigens such as the TSH receptor and the microsomal antigen have not been purified. An alternate approach devised by Young & Davis (1983) is to construct a cDNA library in a vector that expresses the encoded proteins. The library can then be screened with an antibody as a probe. We have constructed cDNA libraries in gt11 using mRNA purified from human, pig and rat thyroid cells. Our experiences in constructing and screening these libraries will be described. The advantages of this system are 1) the protein does not have to be purified, 2) previously unknown antigens may be identified. The disadvantages are 1) lack of specificity with antibody selection, 2) because the cDNA is inserted in the β-galactosidase gene in the vector the antigen is expressed as a fusion protein. This may disturb the tertiary structure of the antigen and alter its antigenicity, 3) cDNA inserts frequently only contain part of the antigen molecule, and may therefore lack important epitopes; polyclonal antibody may therefore be preferable to monoclonal, 4) only 1 in 6 cDNA inserts will be in the correct reading frame for antigen expression, 5) the expressed protein is not glycosylated, 6) antibody absorption or purification is necessary to reduce the high background of antibody binding to bacterial host protein products. Despite these disadvantages the system has been used to clone numerous proteins. Thus far we have been successful in cloning 3 newly recognized autoimmune thyroid disease-related antigens that are of potential importance in further understanding the nature of these diseases.

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