Production of a polyclonal antiserum against the coat protein of Cucurbit yellow stunting disorder crinivirus expressed in Escherichia coli

EPPO Bulletin ◽  
2005 ◽  
Vol 35 (1) ◽  
pp. 99-103 ◽  
Author(s):  
A. C. Cotillon ◽  
C. Desbiez ◽  
S. Bouyer ◽  
C. Wipf-Scheibel ◽  
C. Gros ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Coat Protein ◽  
Polyclonal Antiserum

Expression and assembly of the potato virus Y (PVY) coat protein (CP) in Escherichia coli cells

1993 ◽  
Vol 28 (1) ◽  
pp. 29-35 ◽  
Author(s):  
Yehuda Stram ◽  
Ilan Sela ◽  
Orit Edelbaum ◽  
Edna Tanne ◽  
Miri Karchi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Coat Protein ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Escherichia Coli Cells

scholarly journals Administration of Escherichia coli endotoxin to rat increases liver mass and hepatocyte volume in vivo

1996 ◽  
Vol 313 (2) ◽  
pp. 479-486 ◽  
Author(s):  
Dalong QIAN ◽  
John T. BROSNAN
Keyword(s):  
Escherichia Coli ◽  
Intraperitoneal Administration ◽  
Polyclonal Antiserum ◽  
Metabolic Effects ◽  
Morphometric Study ◽  
Liver Mass ◽  
Hepatic Mass ◽  
Tnf Α ◽  
Factor Α

We have established, in vivo, an increase in liver mass and hepatocyte volume after a single intraperitoneal administration, to fasted rats, of Escherichia coli lipopolysaccharide (0127:B8) at 3 mg/kg. The phenomenon was time- and dose-dependent and could be prevented by treatment with polyclonal antiserum against tumour necrosis factor-α (TNF-α) before the endotoxin injection. Endotoxin caused an increase of 26% in the hepatic mass compared with fasted controls at 24 h. An increase of 27% in the hepatic water content underlay the altered hepatic mass which could not be accounted for by a change in the volume of hepatic blood and/or interstitial fluid (measured in vivo), suggesting an expansion in the hepatocellular volume. This is supported by an increase of 25% in the K+ content of the endotoxic livers. Morphometric study confirmed a 15% increase in hepatocyte volume after endotoxin administration. The data are discussed in the light of possible metabolic effects of increased hepatocyte volume.

Production of recombinant Potato mop-top virus coat protein in Escherichia coli and generation of antisera recognising native virus protein

2003 ◽  
Vol 110 (1) ◽  
pp. 91-97 ◽  
Author(s):  
Valérie Hélias ◽  
Emmanuel Jacquot ◽  
Maryse Guillet ◽  
Yves Le Hingrat ◽  
Danièle Giblot-Ducray
Keyword(s):  
Escherichia Coli ◽  
Coat Protein ◽  
Virus Protein ◽  
Virus Coat Protein ◽  
Native Virus ◽  
Potato Mop Top Virus ◽  
Virus Coat
Sign in / Sign up

Export Citation Format

Share Document