Computer automated cell size and shape analysis in cryomicroscopy

1987 ◽  
Vol 146 (2) ◽  
pp. 209-219 ◽  
Author(s):  
Kenneth R. Diller ◽  
Shanti J. Aggarwal
2018 ◽  
Vol 75 (3) ◽  
pp. 760-771 ◽  
Author(s):  
Owusu F Aidoo ◽  
Chrysantus M Tanga ◽  
Thomson M Paris ◽  
Sandra A Allan ◽  
Samira A Mohamed ◽  
...  

2020 ◽  
Vol 90 (10) ◽  
pp. 1410-1435
Author(s):  
Sojiro Fukuda ◽  
Hajime Naruse

ABSTRACT Hybrid event beds are the deposits from sediment gravity flows that change their rheological behavior through their passage, entraining muddy sediments and damping turbulence. Muddy facies of hybrid event beds are often associated with abundant mud clasts which show a wide variety of size and shape. The variation of clast occurrence in hybrid event beds is expected to preserve the information of entrainment and transport processes of muddy sediments in submarine density currents. However, previous analyses of hybrid event beds have focused on describing the overall clast occurrence rather than the statistical size and shape analyses because traditional shape parameters are incapable of characterizing the complex shape of mud clasts. Here, a new quantitative grain-shape analysis of mud clasts is conducted and allows visualization of the spatial variation of clast size and shape, which suggests the wide variety of origin and transport systems of entrained mud clasts. This new method revises the traditional elliptic Fourier analysis, substituting Fourier power spectra (FPS) for traditional elliptic Fourier descriptors to overcome the mirror-wise shape problem. Further, principal-component analysis is shown to capture significant shape attributes more effectively than traditional shape parameters. The proposed method is applied to mud clasts in sediment-gravity-flow deposits in the lower Pleistocene Otadai Formation, central Japan. Results imply that there are distinctive shape and size differences of mud clasts that are strongly associated with depositional facies rather than the distance from the source. The clasts have a higher angularity than other facies in the debrite intervals in hybrid event beds. It is also shown that clasts in sandy, structureless facies have different characteristics in shapes based on elongation and convexity compared to laminated facies. Comparison between different shape-analysis methods demonstrates that none of the traditional methods are able to visualize these trends as effectively as the method presented herein. These results highlight the importance of the quantitative shape analysis of sediment grains and the effectiveness of FPS-based elliptic Fourier analysis.


Plant Methods ◽  
2009 ◽  
Vol 5 (1) ◽  
pp. 5 ◽  
Author(s):  
Peter A McAtee ◽  
Ian C Hallett ◽  
Jason W Johnston ◽  
Robert J Schaffer

Author(s):  
Denis H. Lynn ◽  
Guy L. Gilron

Fifteen marine strombidiid species were measured and identified after protargol staining of bottle-cast samples collected during an annual study of the ciliate microzooplankton in the Caribbean Sea. Nine of these are described herein as new species. One new species from the genus Tontonia, T. simplicidens sp. nov., is defined, based on the pattern of the girdle and ventral kinety. Six new species of Strombidium, S. bilobum sp. nov., S. eurystomum sp. nov., S. ioanum sp. nov., S. maedai sp. nov., S. pollostomum sp. nov. and S. sphaericum sp. nov. are defined, based on cell size and shape, the arrangement of the oral ciliature, and the nature of the macronucleus. One new strombidiid genus Cyrtostrombidium gen. nov., is defined by the presence of a unique oral ‘basket’ and the absence of the ventral polykinetidal zone. Two species of Cyrtostrombidium, C. longisomum sp. nov. and C. wailesi sp. nov., are distinguished, based on cell size and macronuclear structure. Assemblages of six previously-described strombidiid species, S. constrictum, S. dalum, S. epidemum, S. inclinatum, S. wulffi, and Laboea strobila are also briefly described.


2006 ◽  
Vol 16 (17) ◽  
pp. 1685-1693 ◽  
Author(s):  
Jason Meyers ◽  
Jennifer Craig ◽  
David J. Odde

2006 ◽  
Vol 84 (8) ◽  
pp. 1233-1248 ◽  
Author(s):  
Christopher T. Yarnes ◽  
William J. Boecklen ◽  
Kati Tuominen ◽  
Juha-Pekka Salminen

Interspecific variation in phenolic metabolism across plant species has been correlated to numerous ecological factors, yet generalities concerning the ecological role(s) of phenolics remain elusive. Moreover, studies of allometric variation (absolute and relative concentration) in phenolic metabolism are rare despite the importance of each to ecological interactions. In this study, we characterize individual phenolics in a group of 12 oak species from the Chihuahuan Desert and examine interspecific variation in the absolute and relative concentrations of phenolics using size and shape analysis. Size and shape analysis was able to successfully identify those compounds that contribute most to the interspecific allometric variation in phenolics and classify the oak species on the basis of phenolic metabolism. White versus black oak subgenera were found to be most different in their phenolic composition, where the two black oak species contained fewer and less diverse phenolics. Within the 10 white oak species, a predominantly eastern white oak, Quercus muhlenbergii Engelmann, was found to vary significantly from the more widespread white oaks of the Chihuahuan Desert. We also report the occurrence of complex tannins in oaks of North America for the first time, update proposed pathways of ellagitannin biosynthesis in oaks, and discuss the applicability of size and shape analysis to ecological studies of phenolics.


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