Computer-based image analysis of cartilage differentiation in embryonic limb bud micromass cultures

1989 ◽  
Vol 156 (3) ◽  
pp. 353-361 ◽  
Author(s):  
James C. Garrison ◽  
Paul Peterson ◽  
Edwin M. Uyeki
Author(s):  
Yongchun Zhou ◽  
Junye Liu ◽  
Guozhen Guo ◽  
Kangchu Li ◽  
Jie Zhang ◽  
...  
Keyword(s):  
Limb Bud ◽  

Author(s):  
Sapna Tibrewal ◽  
Peng Tian ◽  
Dharanish Kedarisetti ◽  
Jayashree Kalpathy-Cramer ◽  
Deniz Erdogmus ◽  
...  

2011 ◽  
Vol 25 (1) ◽  
pp. 153-159 ◽  
Author(s):  
Yang Xiao ◽  
Ran Liu ◽  
Lina Xing ◽  
Yajun Xu ◽  
Lanqin Shang ◽  
...  

1991 ◽  
Vol 125 (2) ◽  
pp. 201-211 ◽  
Author(s):  
Banani Bhaumick ◽  
R. Marvin Bala

Abstract. Insulin-like growth factors I and II have been shown differentially to affect the growth and carbohydrate metabolism of differentiating cartilage developed from mouse embryonic limb buds in organ culture. To gain insight into the relative importance of IGF-I and II actions in different stages of development of cartilage we have established a primary culture of differentiating chondrocytes from mouse embryonic limb buds. Trypsin digested limb bud cells from 9-11 day old mouse embryos differentiated into chondrocytes by 5-7 days in culture. At all stages of differentiation, distinct receptors of IGF-I and II were observed. IGF-I stimulated growth and sulphate incorporation of the non-differentiated and differentiated chondrocytes. IGF-II stimulated growth of the non-differentiated cells and had no effect on growth or sulphate incorporation by the differentiated cells. IGF-II, however, stimulated the glucose uptake by the cells at all stages of differentiation. These data confirm our previous suggestion that IGF-I in cartilage is the regulator of growth and differentiation, while IGF-II may be an important regulator of glucose metabolism in the tissue.


2006 ◽  
Vol 144 (3) ◽  
pp. 221-227 ◽  
Author(s):  
J. K. SAINIS ◽  
S. P. SHOUCHE ◽  
S. G. BHAGWAT

Varietal identification is an important aspect of crop research and utilization. Identification using computer-based image analysis could be an alternative to visual identification. However, the effectiveness of image analysis systems needs to be established under various real conditions. Three wheat varieties were sown on three different dates. Variation in the grain size and shape of these varieties, brought about by changes in the environmental conditions, was measured using Comprehensive Image Processing Software (CIPS). Some parameters showed considerable grain-to-grain variation, which was either inherent or due to environmental changes during grain filling. Euclidean distances were calculated using either means of all the parameters (ED1), or using only those parameters that did not show a high coefficient of variation (ED2). For samples of the same variety sown at different times, Euclidean distances were smaller compared with samples of different varieties, indicating that grains of the same variety resembled one another. By using the criterion of minimum Euclidean distance it was possible to distinguish between varieties, in spite of variation in grain shape and size due to environmental conditions. It was possible to identify correctly an unknown sample, taken as a test case.


2012 ◽  
Vol 52 (No. 7) ◽  
pp. 316-323
Author(s):  
P. Čermák ◽  
P. Mazal ◽  
D. Palovčíková ◽  
L. Jankovský

By means of computer-based image analysis it was possible to accelerate and automate a number of originally time-consuming activities in plant pathology and forest protection. In 1997–2005, image analysis was applied to the measuring of the size of colonies of fungi in vitro, feeding mark area, mass measuring of the size of spores and other microscopic objects, assessment of the progress of rot in a stem etc. The paper brings particular examples of the application of image analysis including methodical procedures and examples of research results obtained using Lucia G and Quick Photo Micro programs.


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