Identification of glycosaminoglycan-binding sites within hepatitis C virus envelope glycoprotein E2*

2005 ◽  
Vol 12 (6) ◽  
pp. 584-593 ◽  
Author(s):  
L. V. Olenina ◽  
T. I. Kuzmina ◽  
B. N. Sobolev ◽  
T. E. Kuraeva ◽  
E. F. Kolesanova ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Envelope Glycoprotein ◽  
Binding Sites ◽  
Virus Envelope ◽  
Glycoprotein E2

scholarly journals Identification of Amino Acid Residues in CD81 Critical for Interaction with Hepatitis C Virus Envelope Glycoprotein E2

2000 ◽  
Vol 74 (8) ◽  
pp. 3642-3649 ◽  
Author(s):  
Adrian Higginbottom ◽  
Elizabeth R. Quinn ◽  
Chiung-Chi Kuo ◽  
Mike Flint ◽  
Louise H. Wilson ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Amino Acid ◽  
Envelope Glycoprotein ◽  
Tertiary Structure ◽  
Antibody Binding ◽  
Viral Envelope ◽  
Amino Acid Residues ◽  
Virus Envelope ◽  
Glycoprotein E2

ABSTRACT Human CD81 has been previously identified as the putative receptor for the hepatitis C virus envelope glycoprotein E2. The large extracellular loop (LEL) of human CD81 differs in four amino acid residues from that of the African green monkey (AGM), which does not bind E2. We mutated each of the four positions in human CD81 to the corresponding AGM residues and expressed them as soluble fusion LEL proteins in bacteria or as complete membrane proteins in mammalian cells. We found human amino acid 186 to be critical for the interaction with the viral envelope glycoprotein. This residue was also important for binding of certain anti-CD81 monoclonal antibodies. Mutating residues 188 and 196 did not affect E2 or antibody binding. Interestingly, mutation of residue 163 increased both E2 and antibody binding, suggesting that this amino acid contributes to the tertiary structure of CD81 and its ligand-binding ability. These observations have implications for the design of soluble high-affinity molecules that could target the CD81-E2 interaction site(s).

scholarly journals Structure of Hepatitis C Virus Envelope Glycoprotein E2 Antigenic Site 412 to 423 in Complex with Antibody AP33

2012 ◽  
Vol 86 (23) ◽  
pp. 13085-13088 ◽  
Author(s):  
L. Kong ◽  
E. Giang ◽  
T. Nieusma ◽  
J. B. Robbins ◽  
M. C. Deller ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Envelope Glycoprotein ◽  
Antigenic Site ◽  
Virus Envelope ◽  
Glycoprotein E2

scholarly journals CS-SELEX Generates High-Affinity ssDNA Aptamers as Molecular Probes for Hepatitis C Virus Envelope Glycoprotein E2

PLoS ONE ◽  
2009 ◽  
Vol 4 (12) ◽  
pp. e8142 ◽  
Author(s):  
Fang Chen ◽  
Yilan Hu ◽  
Dongqing Li ◽  
Haidan Chen ◽  
Xiao-Lian Zhang
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Envelope Glycoprotein ◽  
Molecular Probes ◽  
Virus Envelope ◽  
High Affinity ◽  
Glycoprotein E2

A model for the hepatitis C virus envelope glycoprotein E2

2000 ◽  
Vol 40 (3) ◽  
pp. 355-366 ◽  
Author(s):  
Asutosh T. Yagnik ◽  
Armin Lahm ◽  
Annalisa Meola ◽  
Rosa Maria Roccasecca ◽  
Bruno B. Ercole ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Envelope Glycoprotein ◽  
Virus Envelope ◽  
Glycoprotein E2

scholarly journals Identification of Conserved Residues in Hepatitis C Virus Envelope Glycoprotein E2 That Modulate Virus Dependence on CD81 and SRB1 Entry Factors

2014 ◽  
Vol 88 (18) ◽  
pp. 10584-10597 ◽  
Author(s):  
M. Lavie ◽  
S. Sarrazin ◽  
R. Montserret ◽  
V. Descamps ◽  
T. F. Baumert ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Envelope Glycoprotein ◽  
Virus Envelope ◽  
Conserved Residues ◽  
Glycoprotein E2

scholarly journals Identification of New Functional Regions in Hepatitis C Virus Envelope Glycoprotein E2

2010 ◽  
Vol 85 (4) ◽  
pp. 1777-1792 ◽  
Author(s):  
A. Albecka ◽  
R. Montserret ◽  
T. Krey ◽  
A. W. Tarr ◽  
E. Diesis ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Envelope Glycoprotein ◽  
Virus Envelope ◽  
Functional Regions ◽  
Glycoprotein E2

scholarly journals Interaction of hepatitis C virus envelope glycoprotein E2 with the large extracellular loop of tupaia CD81

2009 ◽  
Vol 15 (2) ◽  
pp. 240 ◽  
Author(s):  
Zhan-Fei Tian ◽  
Hong Shen ◽  
Xi-Hua Fu ◽  
Yi-Chun Chen ◽  
Hubert E Blum ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Envelope Glycoprotein ◽  
Extracellular Loop ◽  
Virus Envelope ◽  
Large Extracellular Loop ◽  
Glycoprotein E2

scholarly journals Cellular Binding of Hepatitis C Virus Envelope Glycoprotein E2 Requires Cell Surface Heparan Sulfate

2003 ◽  
Vol 278 (42) ◽  
pp. 41003-41012 ◽  
Author(s):  
Heidi Barth ◽  
Christiane Schäfer ◽  
Mohammed I. Adah ◽  
Fuming Zhang ◽  
Robert J. Linhardt ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Cell Surface ◽  
Heparan Sulfate ◽  
Envelope Glycoprotein ◽  
Virus Envelope ◽  
Glycoprotein E2
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