An effective method for allele-specific sequencing using restriction enzyme and biotinylation (ASSURE B)

1996 ◽  
Vol 5 (4) ◽  
pp. 591-594 ◽  
Author(s):  
DE-XING ZHANG ◽  
G. M. HEWITT
Keyword(s):  
Restriction Enzyme ◽  
Allele Specific ◽  
Allele Specific Sequencing

Detecting multiple cystic fibrosis mutations by polymerase chain reaction-mediated site-directed mutagenesis

1991 ◽  
Vol 37 (5) ◽  
pp. 753-755 ◽  
Author(s):  
K J Friedman ◽  
W E Highsmith ◽  
L M Silverman
Keyword(s):  
Cystic Fibrosis ◽  
Polymerase Chain Reaction ◽  
Restriction Enzyme ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Chain Reaction ◽  
Allele Specific ◽  
Specific Restriction ◽  
Polymerase Chain

Abstract The polymerase chain reaction (PCR) has been applied in a novel manner to detect the multiple mutations causing cystic fibrosis (CF). PCR-mediated site-directed mutagenesis (PSM) has been applied to create allele-specific restriction enzyme cutting sites for three of the more common mutations. Two other mutations after cutting sites on their own. We discuss the implications for the expedient detection of five different CF-causing mutations.

Identification of the CD32/FcγRIIc-Q13/STP13 polymorphism using an allele-specific restriction enzyme digestion assay

2001 ◽  
Vol 258 (1-2) ◽  
pp. 85-95 ◽  
Author(s):  
D Metes ◽  
A.A Gambotto ◽  
J Nellis ◽  
A Ruscin ◽  
A.M Stewart-Akers ◽  
...  
Keyword(s):  
Restriction Enzyme ◽  
Enzyme Digestion ◽  
Restriction Enzyme Digestion ◽  
Allele Specific ◽  
Specific Restriction

NovelNAT2haplotyping using allele-specific sequencing

2014 ◽  
Vol 15 (8) ◽  
pp. 1117-1124 ◽  
Author(s):  
Seong-Ho Kang ◽  
Geon Park ◽  
Sook Jin Jang ◽  
Dae-Soo Moon
Keyword(s):  
Allele Specific ◽  
Allele Specific Sequencing

scholarly journals WASP: allele-specific software for robust discovery of molecular quantitative trait loci

2014 ◽  
Author(s):  
Bryce van de Geijn ◽  
Graham McVicker ◽  
Yoav Gilad ◽  
Jonathan Pritchard
Keyword(s):  
Quantitative Trait Loci ◽  
Qtl Mapping ◽  
Error Rate ◽  
Quantitative Trait ◽  
Rna Seq ◽  
Read Mapping ◽  
Trait Loci ◽  
Allele Specific ◽  
Allele Specific Sequencing

Allele-specific sequencing reads provide a powerful signal for identifying molecular quantitative trait loci (QTLs), however they are challenging to analyze and prone to technical artefacts. Here we describe WASP, a suite of tools for unbiased allele-specific read mapping and discovery of molecular QTLs. Using simulated reads, RNA-seq reads and ChIP-seq reads, we demonstrate that our approach has a low error rate and is far more powerful than existing QTL mapping approaches.

Use of allele‐specific sequencing primers is an efficient alternative to PCR subcloning of low‐copy nuclear genes

2011 ◽  
Vol 12 (1) ◽  
pp. 128-135 ◽  
Author(s):  
ANNE‐CATHRINE SCHEEN ◽  
BERNARD E. PFEIL ◽  
ANNA PETRI ◽  
NAHID HEIDARI ◽  
STEPHAN NYLINDER ◽  
...  
Keyword(s):  
Nuclear Genes ◽  
Allele Specific ◽  
Efficient Alternative ◽  
Allele Specific Sequencing
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