scholarly journals An effective system for detecting Iris yellow spot virus transmission by Thrips tabaci

2009 ◽  
Vol 59 (3) ◽  
pp. 422-428 ◽  
Author(s):  
T. Inoue ◽  
T. Murai ◽  
T. Natsuaki
Keyword(s):  
Virus Transmission ◽  
Thrips Tabaci ◽  
Iris Yellow Spot Virus ◽  
Yellow Spot ◽  
Spot Virus ◽  
Effective System

scholarly journals Transmission of Iris Yellow Spot Virus by Frankliniella fusca and Thrips tabaci (Thysanoptera: Thripidae)

2012 ◽  
Vol 105 (1) ◽  
pp. 40-47 ◽  
Author(s):  
Rajagopalbabu Srinivasan ◽  
Sivamani Sundaraj ◽  
Hanu R. Pappu ◽  
Stan Diffie ◽  
David G. Riley ◽  
...  
Keyword(s):  
Thrips Tabaci ◽  
Iris Yellow Spot Virus ◽  
Yellow Spot ◽  
Spot Virus ◽  
Frankliniella Fusca

scholarly journals Distribution and Transmission of Iris yellow spot virus

Plant Disease ◽  
2001 ◽  
Vol 85 (8) ◽  
pp. 838-842 ◽  
Author(s):  
A. Kritzman ◽  
M. Lampel ◽  
B. Raccah ◽  
A. Gera
Keyword(s):  
Frankliniella Occidentalis ◽  
Thrips Tabaci ◽  
Iris Yellow Spot Virus ◽  
Yellow Spot ◽  
Onion Thrips ◽  
Spot Virus ◽  
Nucleocapsid Gene ◽  
Thrips Species ◽  
Mother Plants ◽  
Thrips Population

Iris yellow spot virus (IYSV), a new tospovirus associated with a disease in onion (Allium cepa) that is known to growers in Israel as “straw bleaching,” was identified and further characterized by host range, serology, electron microscopy, and molecular analysis of the nucleocapsid gene. The transmissibility of IYSV by Thrips tabaci and Frankliniella occidentalis was studied. IYSV was efficiently transmitted by T. tabaci from infected to healthy onion seedlings and leaf pieces. Two biotypes of F. occidentalis, collected from two different locations in Israel, failed to transmit the virus. Surveys to relate the incidence of thrips populations to that of IYSV were conducted in onion fields. They revealed that the onion thrips T. tabaci was the predominant thrips species, and that its incidence was strongly related to that of IYSV. Forty-five percent of the thrips population collected from IYSV-infected onion and garlic fields in Israel transmitted the virus. IYSV was not transmitted to onion seedlings from infected mother plants through the seed, and was not located in bulbs of infected plants.

scholarly journals First Report of Iris yellow spot virus in Onion in Mauritius

Plant Disease ◽  
2010 ◽  
Vol 94 (11) ◽  
pp. 1373-1373 ◽  
Author(s):  
K. Lobin ◽  
A. Saison ◽  
B. Hostachy ◽  
S. P. Benimadhu ◽  
H. R. Pappu
Keyword(s):  
Disease Incidence ◽  
Consensus Sequence ◽  
Thrips Tabaci ◽  
Iris Yellow Spot Virus ◽  
Yellow Spot ◽  
N Gene ◽  
Spot Virus ◽  
Viral Pathogen ◽  
First Report ◽  
Das Elisa

Iris yellow spot virus (IYSV; family Bunyaviridae, genus Tospovirus) transmitted by thrips (Thrips tabaci Lindeman) is an economically important viral pathogen of bulb and seed onion (Allium cepa) crops in many onion-growing areas of the world (2,3). In Africa, IYSV has been reported in Reunion (4) and South Africa (1). In June 2008, diamond-shaped lesions that are typical of IYSV were observed on onion seed scapes in an onion plot of 0.25 ha at Reduit in the central part of Mauritius. Disease incidence was 80% with a severity of 50 to 75% of the scape surface area. Lodging was observed in 25% of the symptomatic plants. Twenty-two symptomatic plants were tested and found to be positive for IYSV when tested by double antibody sandwich (DAS)-ELISA with a commercially available kit (Agdia Inc., Elkhart, IN). The presence of the virus was confirmed by reverse transcription (RT)-PCR tests with primers 917L: 5′-TAAAACTTAACTAACACAAA-3′ and 56U: 5′-TCCTAAGTATTCACCAT-3′ as forward and reverse primers, respectively, for specific sequences flanking the CP gene. Another set of primers specific to the small (S) RNA of IYSV (5′-TAAAACAAACATTCAAACAA-3′ and 5′-CTCTTAAACACATTT AACAAGCAC-3′) produced an amplicon of approximately 1.2 kb that includes the 772-bp nucleocapsid (N) gene. The 1.2-kb amplicon was cloned and four clones were sequenced and consensus sequence was used for comparisons. Sequence analysis showed that the N gene of the IYSV isolate from Mauritius (GenBank Accession No. HM218822) shared the highest nucleotide sequence identity (99%) with several known IYSV N gene sequences (Accession Nos. FJ785835 and AM900393) available in the GenBank, confirming the presence of IYSV in the onion crops in Mauritius. A survey was subsequently carried out from July to November 2008 in major onion-growing localities at La Marie, Henrietta, Reduit, and Plaine Sophie (center); Bassin, La Ferme, and La Chaumiere (west); Grand Sable, Petit Sable, and Plaisance (south, southeast); and Belle Mare, Trou d'Eau Douce, and Palmar (east) to monitor the distribution of the disease on the island. Symptomatic samples with diamond-to-irregularly shaped lesions were observed and 155 symptomatic and 35 nonsymptomatic samples were collected and screened by DAS-ELISA for IYSV and Tomato spotted wilt virus (TSWV), another tospovirus reported to infect onion elsewhere. Sixty-six percent of the symptomatic samples screened (102 of 155) tested positive for IYSV. No IYSV was detected in the symptomless samples. There was no serological indication of TSWV infection in the samples. Samples that tested positive for IYSV were collected from Belle mare, Palmar, and Trou d'eau douce in the east and La Ferme in the west. Cultivars infected were Gandiole, Local Red, and Veronique. No IYSV was detected in the bulbs. The vector, T. tabaci, was observed in infected onion parcels surveyed and is known to occur in all onion-producing areas of the island. To our knowledge, this is the first report of IYSV in onion in Mauritius. Further surveys and monitoring of IYSV incidence, along with its impact on the yield, need to be established. References: (1) L. J. du Toit et al. Plant Dis. 91:1203, 2007. (2) D. H. Gent et al. Plant Dis. 88:446, 2004. (3) H. R. Pappu et al. Virus Res. 141:219, 2009. (4) I. Robène-Soustrade et al. Plant Pathol. 55:288, 2006.

Iris yellow spot virus. [Distribution map].

2009 ◽  
Author(s):  
Keyword(s):  
New York ◽  
Thrips Tabaci ◽  
Iris Yellow Spot Virus ◽  
Yellow Spot ◽  
Allium Porrum ◽  
Distribution Map ◽  
Spot Virus ◽  
South Wales ◽  
Virus Distribution ◽  
Distribution In Europe

Abstract A new distribution map is provided for Iris yellow spot virus, Bunyaviridae: Tospovirus. Hosts: onion (Allium cepa), Dutch iris (Iris hollandica), leek (Allium porrum) and Hippeastrum spp. Information is given on the geographical distribution in Europe (France, Mainland France, Germany, Italy, Mainland Italy, Netherlands, Poland, Serbia, Slovenia, Spain, Mainland Spain, UK, England and Wales), Asia (India, Maharashtra, Iran, Israel, Japan, Honshu, Kyushu), Africa (Reunion, South Africa, Tunisia), North America (Canada, Ontario, USA, Arizona, California, Colorado, Georgia, Idaho, Nevada, New Mexico, New York, Oregon, Texas, Utah, Washington), Central America and Caribbean (Guatemala), South America (Brazil, Pernambuco, Sao Paulo, Chile, Peru), Oceania (Australia, New South Wales, Victoria, Western Australia, New Zealand). It is vectored by Thrips tabaci (Thysanoptera: Thripidae).

scholarly journals Evaluation of Lisianthus as an Indicator Host for Iris yellow spot virus

Plant Disease ◽  
2011 ◽  
Vol 95 (12) ◽  
pp. 1520-1527 ◽  
Author(s):  
Rajagopalbabu Srinivasan ◽  
Stan Diffie ◽  
Sivamani Sundaraj ◽  
Stephen W. Mullis ◽  
David Riley ◽  
...  
Keyword(s):  
Transmission Rate ◽  
Thrips Tabaci ◽  
Iris Yellow Spot Virus ◽  
Yellow Spot ◽  
Effect Of Temperature ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mechanical Transmission ◽  
Symptom Expression ◽  
Spot Virus ◽  
Temperature Regimes

Iris yellow spot virus (IYSV) can severely affect onion production. IYSV is transmitted by the onion thrips, Thrips tabaci. However, information on IYSV–thrips–onion interactions is limited due to the difficulty associated with infecting onion plants experimentally. Lisianthus (Eustoma russellianum) was used as an indicator host to study mechanical transmission of IYSV, IYSV transmission by T. tabaci, IYSV distribution in the host plant, and the effect of temperature on IYSV symptom expression. Mechanical inoculation tests from IYSV-infected onion plants to noninfected lisianthus plants resulted in a mean transmission rate of 82.5 ± 6.9% (mean ± standard error), and from IYSV-infected lisianthus plants to noninfected lisianthus plants resulted in a mean transmission rate of 89.2 ± 7.1%. T. tabaci adults transmitted IYSV at a rate of 80.0 ± 8.3% from infected onion plants to noninfected lisianthus plants. To assess IYSV distribution in infected lisianthus plants, leaf sections, stems, and roots were tested by enzyme-linked immunosorbent assay (ELISA). All the plant parts tested positive for IYSV, but not on every plant assayed. Alternating night and day temperatures of 18 and 23°C, 25 and 30°C, and 30 and 37°C were evaluated for the effects on IYSV symptom expression. More severe symptoms developed on inoculated plants incubated at the 18 and 23°C or 25 and 30°C temperature regimes than at the 30 and 37°C regime, and symptoms were observed earliest on plants incubated at the 25 and 30°C temperature regime compared to the other temperature regimes.

Sign in / Sign up

Export Citation Format

Share Document