A forward genetic screen to explore chloroplast protein import in vivo identifies Moco sulfurase, pivotal for ABA and IAA biosynthesis and purine turnover

2010 ◽  
pp. no-no ◽  
Author(s):  
Rong Zhong ◽  
Jennifer Thompson ◽  
Eric Ottesen ◽  
Gayle K. Lamppa
Keyword(s):  
Protein Import ◽  
Genetic Screen ◽  
Forward Genetic Screen ◽  
Chloroplast Protein Import ◽  
Chloroplast Protein ◽  
Iaa Biosynthesis

scholarly journals In vivo studies on the roles of Tic110, Tic40 and Hsp93 during chloroplast protein import

2004 ◽  
Vol 41 (3) ◽  
pp. 412-428 ◽  
Author(s):  
Sabina Kovacheva ◽  
Jocelyn Bédard ◽  
Ramesh Patel ◽  
Penny Dudley ◽  
David Twell ◽  
...  
Keyword(s):  
Protein Import ◽  
In Vivo Studies ◽  
Chloroplast Protein Import ◽  
Chloroplast Protein

scholarly journals In vivo Studies on the Roles of Tic55-Related Proteins in Chloroplast Protein Import in Arabidopsis thaliana

2009 ◽  
Vol 2 (6) ◽  
pp. 1397-1409 ◽  
Author(s):  
Patrik Boij ◽  
Ramesh Patel ◽  
Christel Garcia ◽  
Paul Jarvis ◽  
Henrik Aronsson
Keyword(s):  
Arabidopsis Thaliana ◽  
Protein Import ◽  
In Vivo Studies ◽  
Chloroplast Protein Import ◽  
Chloroplast Protein ◽  
Related Proteins

scholarly journals Massively parallel in vivo CRISPR screening identifies RNF20/40 as epigenetic regulators of cardiomyocyte maturation

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Nathan J. VanDusen ◽  
Julianna Y. Lee ◽  
Weiliang Gu ◽  
Catalina E. Butler ◽  
Isha Sethi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Genetic Screen ◽  
Forward Genetics ◽  
Epigenetic Mark ◽  
Biological Processes ◽  
Dynamic Changes ◽  
Forward Genetic Screen ◽  
High Resource ◽  
Organ Systems

AbstractThe forward genetic screen is a powerful, unbiased method to gain insights into biological processes, yet this approach has infrequently been used in vivo in mammals because of high resource demands. Here, we use in vivo somatic Cas9 mutagenesis to perform an in vivo forward genetic screen in mice to identify regulators of cardiomyocyte (CM) maturation, the coordinated changes in phenotype and gene expression that occur in neonatal CMs. We discover and validate a number of transcriptional regulators of this process. Among these are RNF20 and RNF40, which form a complex that monoubiquitinates H2B on lysine 120. Mechanistic studies indicate that this epigenetic mark controls dynamic changes in gene expression required for CM maturation. These insights into CM maturation will inform efforts in cardiac regenerative medicine. More broadly, our approach will enable unbiased forward genetics across mammalian organ systems.

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