Evaluation of chimerism by quantitative PCR analysis of DNA polymorphism after allogeneic hematopoietic stem cell transplantation in a pediatric population with malignancies

2010 ◽  
Vol 15 (1) ◽  
pp. 81-87
Author(s):  
F. Bautista ◽  
L. Moreno ◽  
J. M. Fernández-Navarro ◽  
L. Senent ◽  
M. M. Andrés ◽  
...  
Keyword(s):  
Stem Cell ◽  
Hematopoietic Stem Cell Transplantation ◽  
Stem Cell Transplantation ◽  
Cell Transplantation ◽  
Quantitative Pcr ◽  
Hematopoietic Stem Cell ◽  
Dna Polymorphism ◽  
Pediatric Population ◽  
Pcr Analysis ◽  
Hematopoietic Stem

scholarly journals Unrelated hematopoietic stem cell transplantation in the pediatric population: single institution experience

2015 ◽  
Vol 37 (4) ◽  
pp. 236-241 ◽  
Author(s):  
Daniela Hespanha Marinho ◽  
José Zanis Neto ◽  
Carmem Maria Sales Bonfim ◽  
Vaneuza Araujo Moreira Funke ◽  
Lisandro Lima Ribeiro
Keyword(s):  
Stem Cell ◽  
Hematopoietic Stem Cell Transplantation ◽  
Stem Cell Transplantation ◽  
Cell Transplantation ◽  
Hematopoietic Stem Cell ◽  
Pediatric Population ◽  
Single Institution ◽  
Hematopoietic Stem

Role of fibrinolytic and clotting parameters in the diagnosis of liver veno-occlusive disease after hematopoietic stem cell transplantation in a pediatric population

2005 ◽  
Vol 93 (04) ◽  
pp. 682-689 ◽  
Author(s):  
Luca Spiezia ◽  
Simone Cesaro ◽  
Chiara Messina ◽  
Myriam Paris ◽  
Marta Pillon ◽  
...  
Keyword(s):  
Stem Cell ◽  
Hematopoietic Stem Cell Transplantation ◽  
Stem Cell Transplantation ◽  
Cell Transplantation ◽  
Hematopoietic Stem Cell ◽  
Pediatric Population ◽  
Occlusive Disease ◽  
Plasminogen Activator Inhibitor 1 ◽  
Hematopoietic Stem ◽  
Pai 1

SummaryHepatic veno-occlusive disease (VOD) is a severe complication after hematopoietic stem cell transplantation (HSCT). Recent studies, mainly in adults receiving HSCT, have identified an increase in the plasminogen activator inhibitor-1 (PAI-1) as a possible marker of VOD. To confirm this finding, the fibrinolytic, coagulation and liver function parameters were assayed before and weekly for 1 month after 61 HSCT performed in 53 consecutive children. Non-VOD patients had a slight increase in t-PA antigen, fibrinogen and P-selectin levels, as well as a mildly longer aPTT and a drop in antithrombin after HSCT. The 6 children with VOD (9.84%) had an early and significant increase in PAI-1 antigen and activity (p< 0.0001), t-PA antigen (p< 0.0001) and D-dimer (p< 0.01) levels, and a decrease in plasminogen, α2-antiplasmin and PT emerged 2(±1) days before the clinical diagnosis of VOD by comparison with mean post-HSCT values in the non-VOD patients. Significant differences were also detected for these parameters and antithrombin levels between non-VOD and VOD patients soon after the clinical onset of VOD, whereas the rise in bilirubin levels became significant only later on. In conclusion, variations in fibrinolytic test findings after HSCT, and PAI-1 in particular, may facilitate the early diagnosis of VOD in pediatric patients after HSCT.

scholarly journals Quantitative PCR technology in chimerism status evaluation after hematopoietic stem cell transplantation

2019 ◽  
Vol 2 (1) ◽  
pp. 44-47
Author(s):  
Katarina Stingl Jankovic
Keyword(s):  
Stem Cell ◽  
Hematopoietic Stem Cell Transplantation ◽  
Stem Cell Transplantation ◽  
Cell Transplantation ◽  
Quantitative Pcr ◽  
Hematopoietic Stem Cell ◽  
Disease Relapse ◽  
Hematopoietic Stem ◽  
Qpcr Method ◽  
Pcr Technology

Chimerism status evaluation is one of the most useful methods for the assessment of the hematopoietic stem cell transplantation outcome, since it gives information about engraftment and disease relapse, as well as providing indication for timely interventions such as donor lymphocyte infusions. The aim of this article is to give an overview of the different methods used for chimerism detection, with a especial emphasis on the quantitative PCR (qPCR) based techniques as the newest in line of techniques used for chimerism monitoring. This overview covers a description of the qPCR method as well as a discussion about the advantages this technique offers in comparison to other methods.

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