Esterified cholesterol and triglyceride are present in plasma membranes of Chinese hamster ovary cells

Growth of Chinese-hamster ovary cells in [32P]phosphate and [3H]leucine and subsequent assay of the plasma membranes reveals phosphorylation in two protein regions corresponding to molecular weights of 280000 and 195000. Culture in the presence of the 6-N,2-O′-dibutyryl derivative of cyclic AMP plus testosterone does not stimulate [32P]-phosphate incorporation, but determines a modification in the qualitative pattern of phosphorylation.

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The Isolation and Characterization of the Plasma Membrane of Cultured Chinese Hamster Ovary Cells

Canadian Journal of Biochemistry ◽

10.1139/o75-126 ◽

1975 ◽

Vol 53 (8) ◽

pp. 914-920 ◽

Cited By ~ 8

Author(s):

R. L. Juliano ◽

E. Gagalang

Keyword(s):

Plasma Membrane ◽

Chinese Hamster Ovary ◽

Plasma Membranes ◽

Chinese Hamster Ovary Cells ◽

Polymer System ◽

Chinese Hamster ◽

Two Phase ◽

Ovary Cells ◽

Isolation And Characterization ◽

Chemical Assay

Plasma membranes were prepared from cultured Chinese hamster ovary cells utilizing a two-phase polymer system and were characterized by enzymatic and chemical assay, and by electron microscopy. The usual degree of purification of presumptive membrane markers such as Na+–K+ ATPase (ATP phosphohydrolase, EC 3.6.1.3) ranged from three-to eightfold. Gel electrophoresis in SDS revealed several polypeptides and two glycopeptides which were enriched in the plasma membrane fraction.

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Malignancy-related characteristics of wild type and drug-resistant chinese hamster ovary cells

Pathology ◽

10.3109/00313029309066588 ◽

1993 ◽

Vol 25 (3) ◽

pp. 268-276 ◽

Cited By ~ 5

Author(s):

Wanda B. Mackinnon ◽

Marlen Dyne ◽

Rebecca Hancock ◽

Carolyn E. Mountford ◽

Adrienne J. Grant ◽

...

Keyword(s):

Chinese Hamster Ovary ◽

Chinese Hamster Ovary Cells ◽

Chinese Hamster ◽

Drug Resistant ◽

Wild Type ◽

Ovary Cells

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Faculty Opinions recommendation of Integrin alphaIIbbeta3 activation in Chinese hamster ovary cells and platelets increases clustering rather than affinity.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.2712967.2376067 ◽

2010 ◽

Author(s):

Myron Cybulsky ◽

Sharon Hyduk

Keyword(s):

Chinese Hamster Ovary ◽

Chinese Hamster Ovary Cells ◽

Chinese Hamster ◽

Ovary Cells

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Review of the current methods of Chinese Hamster Ovary (CHO) cells cultivation for production of therapeutic protein

Current Drug Discovery Technologies ◽

10.2174/1570163817666200312102137 ◽

2020 ◽

Vol 17 ◽

Cited By ~ 3

Author(s):

Shazid Md. Sharker ◽

Md. Atiqur Rahman

Keyword(s):

Chinese Hamster Ovary ◽

Cho Cells ◽

Mass Production ◽

Chinese Hamster Ovary Cells ◽

Chinese Hamster ◽

Therapeutic Protein ◽

Specific Productivity ◽

Ovary Cells ◽

Further Development ◽

Interesting Area

Most of clinical approved protein-based drugs or under in clinical trial have a profound impact in the treatment of critical diseases. The mammalian eukaryotic cells culture approaches, particularly the CHO (Chinese Hamster Ovary) cells are mainly used in the biopharmaceutical industry for the mass-production of therapeutic protein. Recent advances in CHO cell bioprocessing to yield recombinant proteins and monoclonal antibodies have enabled the expression of quality protein. The developments of cell lines are possible to upgrade specific productivity. As a result, it holds an interesting area for academic as well as industrial researchers around the world. This review will concentrate on the recent progress of the mammalian CHO cells culture technology and the future scope of further development for the mass-production of protein therapeutics.

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