The Isolation and Characterization of the Plasma Membrane of Cultured Chinese Hamster Ovary Cells
Keyword(s):
Plasma membranes were prepared from cultured Chinese hamster ovary cells utilizing a two-phase polymer system and were characterized by enzymatic and chemical assay, and by electron microscopy. The usual degree of purification of presumptive membrane markers such as Na+–K+ ATPase (ATP phosphohydrolase, EC 3.6.1.3) ranged from three-to eightfold. Gel electrophoresis in SDS revealed several polypeptides and two glycopeptides which were enriched in the plasma membrane fraction.
1979 ◽
Vol 101
(1)
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pp. 149-156
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1993 ◽
Vol 4
(2)
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pp. 130-140
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1975 ◽
Vol 86
(2)
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pp. 253-260
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1992 ◽
Vol 205
(2)
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pp. 827-839
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Keyword(s):
1995 ◽
Vol 187
(1)
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pp. 111-119
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1988 ◽
Vol 939
(2)
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pp. 247-259
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1993 ◽
Vol 268
(14)
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pp. 10145-10153