scholarly journals Disruption of the gene encoding the NADH-binding subunit of NADH: ubiquinone oxidoreductase in Neurospora crassa. Formation of a partially assembled enzyme without FMN and the iron-sulphur cluster N-3

1994 ◽  
Vol 220 (2) ◽  
pp. 551-558 ◽  
Author(s):  
Wolfgang FECKE ◽  
Vladimir D. SLED ◽  
Tomoko OHNISHI ◽  
Hanns WEISS
1998 ◽  
Vol 76 (1) ◽  
pp. 139-143 ◽  
Author(s):  
Paulo Caseiro Alves ◽  
Arnaldo Videira

The assembly of mitochondrial NADH : ubiquinone oxidoreductase (complex I) was studied in the E35 stopper mutant of Neurospora crassa at different times during growth in liquid media. Assembly of complex I as well as of its membrane domain is impaired in this strain throughout the growth period. Nevertheless, a structure that resembles the peripheral arm of the enzyme is still formed in the mitochondria of this mutant. The absence of the membrane domain of complex I in E35 can be attributed to the specific deletion of the mitochondrial ND2 and ND3 subunits of the enzyme.Key words: mitochondria, complex I, stopper mutants, Neurospora crassa.


1992 ◽  
Vol 20 (3) ◽  
pp. 395-404 ◽  
Author(s):  
Najat Haouazine ◽  
Anete de Souza Pereira ◽  
Marie-France Jubier ◽  
Dominique Lancelin ◽  
Evelyne Delcher ◽  
...  

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