scholarly journals Isolation and inactivation of the nuclear gene encoding the rotenone-insensitive internal NADH: ubiquinone oxidoreductase of mitochondria from Saccharomyces cerevisiae

1991 ◽  
Vol 195 (3) ◽  
pp. 857-862 ◽  
Author(s):  
C. A. M. MARRES ◽  
S. VRIES ◽  
L. A. GRIVELL
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nuclear Gene ◽  
Gene Encoding ◽  
Ubiquinone Oxidoreductase ◽  
Nadh Ubiquinone Oxidoreductase

Saccharomyces cerevisiae contains two functional citrate synthase genes

1986 ◽  
Vol 6 (6) ◽  
pp. 1936-1942
Author(s):  
K S Kim ◽  
M S Rosenkrantz ◽  
L Guarente
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Carbon Source ◽  
Citrate Synthase ◽  
Tricarboxylic Acid Cycle ◽  
Nuclear Gene ◽  
Tricarboxylic Acid ◽  
Yeast Genome ◽  
Gene Encoding ◽  
Acid Cycle ◽  
Nonfermentable Carbon Source

The tricarboxylic acid cycle occurs within the mitochondria of the yeast Saccharomyces cerevisiae. A nuclear gene encoding the tricarboxylic acid cycle enzyme citrate synthase has previously been isolated (M. Suissa, K. Suda, and G. Schatz, EMBO J. 3:1773-1781, 1984) and is referred to here as CIT1. We report here the isolation, by an immunological method, of a second nuclear gene encoding citrate synthase (CIT2). Disruption of both genes in the yeast genome was necessary to produce classical citrate synthase-deficient phenotypes: glutamate auxotrophy and poor growth on rich medium containing lactate, a nonfermentable carbon source. Therefore, the citrate synthase produced from either gene was sufficient for these metabolic roles. Transcription of both genes was maximally repressed in medium containing both glucose and glutamate. However, transcription of CIT1 but not of CIT2 was derepressed in medium containing a nonfermentable carbon source. The significance of the presence of two genes encoding citrate synthase in S. cerevisiae is discussed.

The wheat mitochondrial genome contains an ORF showing sequence homology to the gene encoding the subunit 6 of the NADH-ubiquinone oxidoreductase

1992 ◽  
Vol 20 (3) ◽  
pp. 395-404 ◽  
Author(s):  
Najat Haouazine ◽  
Anete de Souza Pereira ◽  
Marie-France Jubier ◽  
Dominique Lancelin ◽  
Evelyne Delcher ◽  
...  
Keyword(s):  
Mitochondrial Genome ◽  
Sequence Homology ◽  
Gene Encoding ◽  
Ubiquinone Oxidoreductase ◽  
Nadh Ubiquinone Oxidoreductase

The NAM9-1 suppressor mutation in a nuclear gene encoding ribosomal mitochondrial protein of Saccharomyces cerevisiae

Gene ◽  
1995 ◽  
Vol 162 (1) ◽  
pp. 81-85 ◽  
Author(s):  
Aleksandra Dmochowska ◽  
Agata Konopińska ◽  
Magdalena Krzymowska ◽  
Barbara Szcześniak ◽  
Magdalena Boguta
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mitochondrial Protein ◽  
Nuclear Gene ◽  
Suppressor Mutation ◽  
Gene Encoding

scholarly journals The PET54 gene of Saccharomyces cerevisiae: characterization of a nuclear gene encoding a mitochondrial translational activator and subcellular localization of its product.

Genetics ◽  
1989 ◽  
Vol 122 (2) ◽  
pp. 297-305 ◽  
Author(s):  
M C Costanzo ◽  
E C Seaver ◽  
T D Fox
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nuclear Gene ◽  
Open Reading Frames ◽  
Gene Encoding ◽  
Reading Frame ◽  
Acid Protein ◽  
Translational Activator ◽  
The Right ◽  
A Minor

Abstract The product of the nuclear Saccharomyces cerevisiae gene PET54 is specifically required, along with at least two other nuclear gene products, for translation of the mitochondrial mRNA encoding subunit III of cytochrome c oxidase (coxIII). We have genetically mapped PET54 (to the right arm of chromosome VII, 4.8 cM centromere-distal to SUF15), and have biochemically characterized the gene and its product. We determined the nucleotide sequence of a 1.6-kb DNA fragment carrying PET54 and identified the PET54 reading frame by determining the sequence of an ochre mutant allele as well as frameshift and frameshift-revertant alleles of the gene. The wild-type PET54 gene encodes a slightly basic 293-amino acid protein. PET54 is expressed from two mRNAs, both with unusual features: a major transcript with an extremely short 5'-untranslated leader, and a minor transcript with a relatively long 5'-leader carrying three short open reading frames. Antiserum raised against a trpE-PET54 fusion protein was used to probe subcellular fractions. These experiments showed that the PET54 protein is specifically associated with mitochondria, suggesting that it is likely to act directly in coxIII translation.

scholarly journals Saccharomyces cerevisiae contains two functional citrate synthase genes.

1986 ◽  
Vol 6 (6) ◽  
pp. 1936-1942 ◽  
Author(s):  
K S Kim ◽  
M S Rosenkrantz ◽  
L Guarente
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Carbon Source ◽  
Citrate Synthase ◽  
Tricarboxylic Acid Cycle ◽  
Nuclear Gene ◽  
Tricarboxylic Acid ◽  
Yeast Genome ◽  
Gene Encoding ◽  
Acid Cycle ◽  
Nonfermentable Carbon Source

The tricarboxylic acid cycle occurs within the mitochondria of the yeast Saccharomyces cerevisiae. A nuclear gene encoding the tricarboxylic acid cycle enzyme citrate synthase has previously been isolated (M. Suissa, K. Suda, and G. Schatz, EMBO J. 3:1773-1781, 1984) and is referred to here as CIT1. We report here the isolation, by an immunological method, of a second nuclear gene encoding citrate synthase (CIT2). Disruption of both genes in the yeast genome was necessary to produce classical citrate synthase-deficient phenotypes: glutamate auxotrophy and poor growth on rich medium containing lactate, a nonfermentable carbon source. Therefore, the citrate synthase produced from either gene was sufficient for these metabolic roles. Transcription of both genes was maximally repressed in medium containing both glucose and glutamate. However, transcription of CIT1 but not of CIT2 was derepressed in medium containing a nonfermentable carbon source. The significance of the presence of two genes encoding citrate synthase in S. cerevisiae is discussed.

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