Evolution of Gene Regulation as Revealed by Differential Regulation of the Chicken Lysozyme Transgene and the Endogenous Mouse Lysozyme Gene in Mouse Macrophages

Having role in gene regulation and silencing, miRNAs have been implicated in development and progression of a number of diseases, including cancer. Herein, I present potential miRNAs associated with BAP1 gene identified using in-silico tools such as TargetScan and Exiqon miRNA Target Prediction. I identified fifteen highly conserved miRNA (hsa-miR-423-5p, hsa-miR-3184-5p, hsa-miR-4319, hsa-miR125b-5p, hsa-miR-125a-5p, hsa-miR-6893-3p, hsa-miR-200b-3p, hsa-miR-200c-3p, hsa-miR-505-3p.1, hsa-miR-429, hsa-miR-370-3p, hsa-miR-125a-5p, hsa-miR-141-3p, hsa-miR-200a-3p, and hsa-miR-429) associated with BAP1 gene. We also predicted the differential regulation of these twelve miRNAs in different cancer types.

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Faculty Opinions recommendation of The evolution of gene regulation underlies a morphological difference between two Drosophila sister species.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1104214.569118 ◽

2008 ◽

Author(s):

Yoav Gilad

Keyword(s):

Gene Regulation ◽

Morphological Difference ◽

Sister Species ◽

Evolution Of Gene Regulation

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Characterization of a myeloid-specific enhancer of the chicken lysozyme gene. Major role for an Ets transcription factor-binding site.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)32378-5 ◽

1994 ◽

Vol 269 (27) ◽

pp. 17794-17801

Author(s):

B. Ahne ◽

W.H. Strätling

Keyword(s):

Transcription Factor ◽

Binding Site ◽

Transcription Factor Binding Site ◽

Transcription Factor Binding ◽

Factor Binding Site ◽

Factor Binding ◽

Lysozyme Gene ◽

Ets Transcription Factor ◽

Chicken Lysozyme

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The far upstream chicken lysozyme enhancer at -6.1 kilobase, by interacting with NF-M, mediates lipopolysaccharide-induced expression of the chicken lysozyme gene in chicken myelomonocytic cells.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)47423-6 ◽

1994 ◽

Vol 269 (49) ◽

pp. 31302-31309

Author(s):

R Goethe ◽

P V Loc

Keyword(s):

Induced Expression ◽

Lysozyme Gene ◽

Myelomonocytic Cells ◽

Chicken Lysozyme

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Evolution of gene regulation between ascidian species

Mechanisms of Development ◽

10.1016/j.mod.2017.04.287 ◽

2017 ◽

Vol 145 ◽

pp. S108-S109

Author(s):

Alicia Madgwick ◽

Damien Gailly ◽

Marta Silvia Magri ◽

José-Luis Gomez-Skarmeta ◽

Patrick Lemaire

Keyword(s):

Gene Regulation ◽

Ascidian Species ◽

Evolution Of Gene Regulation

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Secretion of foreign proteins mediated by chicken lysozyme gene regulatory sequences

Biochemistry and Cell Biology ◽

10.1139/o02-163 ◽

2002 ◽

Vol 80 (6) ◽

pp. 777-788 ◽

Cited By ~ 4

Author(s):

Gregory R Lampard ◽

Ann M. Verrinder Gibbins

Keyword(s):

Fluorescent Protein ◽

Regulatory Sequences ◽

Heterologous Proteins ◽

Transgenic Chicken ◽

Reporter Protein ◽

Lysozyme Gene ◽

Foreign Proteins ◽

Gene Regulatory ◽

Chicken Lysozyme

Exploitation of the insulating properties of the complete chicken lysozyme gene domain may facilitate the production of transgenic chicken bioreactors with the capacity to deposit valuable proteins in the egg white. Chimeric genes consisting of the chicken lysozyme gene regulatory sequences and sequences encoding foreign proteins could be inserted randomly into the chicken genome and retain appropriate expression levels. The research reported here established that chicken lysozyme gene regulatory sequences can be used to direct the production and secretion of green fluorescent protein (used as a reporter protein) in transiently transfected chicken blastodermal cells. Attempts to verify these findings in transgenic hens are currently in progress. To provide a rapid means of generating constructs encoding other foreign proteins under the control of lysozyme gene regulatory sequences that can facilitate the secretion of heterologous proteins in vivo, a generic lysozyme gene regulatory scaffold was created using a poxvirus-mediated gene targeting system.Key words: chicken lysozyme gene, secretion, homologous recombination.

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Transcriptional activation of the chicken lysozyme gene by NF-κ Bp65 (RelA) and c-Rel, but not by NF-κ Bp50

Biochemical Journal ◽

10.1042/bj3130039 ◽

1996 ◽

Vol 313 (1) ◽

pp. 39-44 ◽

Cited By ~ 25

Author(s):

Loc VAN PHI

Keyword(s):

Transcription Factors ◽

Transcriptional Activation ◽

Nuclear Factor Kappa B ◽

Protein Complexes ◽

Terminal Differentiation ◽

Binding Activity ◽

Nuclear Factor ◽

Lysozyme Gene ◽

Chloramphenicol Acetyltransferase Gene ◽

Chicken Lysozyme

The lysozyme gene is expressed at a low level in myeloblasts and is progressively activated to constitutively high expression in mature macrophages. The binding activity of the newly defined NF-ĸB/Rel family of transcription factors increases during the terminal differentiation of macrophages. In this study, I show that NF-ĸB/Rel-like proteins bind to the nuclear factor kappa B (ĸB)-like sequence of the lysozyme promoter. These binding activities were induced by treatment of HD11 cells with lipopolysaccharide. Immunomobility shift assays show that c-Rel is possibly a factor in the complexes that bind to the ĸB-like sequence lysĸB. Binding activity to one of the protein complexes seems to be regulated by phosphorylation. In fact, overexpression of p65 and c-Rel stimulates expression of the chloramphenicol acetyltransferase gene controlled by the lysozyme promoter. Furthermore, co-transfection experiments reveal that the ĸB-like sequence within the lysozyme promoter mediates the transactivation by p65 and c-Rel. These results indicate that the p65 and c-Rel could be components of the protein complexes that bind to the ĸB-like sequence and this binding could contribute to the progressively activated expression of the lysozyme gene during the terminal differentiation of macrophages.

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