scholarly journals Characterization of a Life-Cycle-Stage-Regulated Membrane Protein Tyrosine Phosphatase in Trypanosoma Brucei

1995 ◽  
Vol 234 (3) ◽  
pp. 871-877 ◽  
Author(s):  
Norbert Bakalara ◽  
Andreas Seyfang ◽  
Charles Davis ◽  
Theo Baltz
Keyword(s):  
Life Cycle ◽  
Membrane Protein ◽  
Trypanosoma Brucei ◽  
Protein Tyrosine Phosphatase ◽  
Tyrosine Phosphatase ◽  
Life Cycle Stage ◽  
Protein Tyrosine

scholarly journals Protein tyrosine phosphatase TbPTP1: a molecular switch controlling life cycle differentiation in trypanosomes

2006 ◽  
Vol 175 (2) ◽  
pp. 293-303 ◽  
Author(s):  
Balázs Szöőr ◽  
Jude Wilson ◽  
Helen McElhinney ◽  
Lydia Tabernero ◽  
Keith R. Matthews
Keyword(s):  
Life Cycle ◽  
Trypanosoma Brucei ◽  
Protein Tyrosine Phosphatase ◽  
Tyrosine Phosphatase ◽  
Tsetse Fly ◽  
Mammalian Host ◽  
African Trypanosomes ◽  
Protein Tyrosine ◽  
Ptp1b Inhibitors ◽  
Stumpy Form

Differentiation in African trypanosomes (Trypanosoma brucei) entails passage between a mammalian host, where parasites exist as a proliferative slender form or a G0-arrested stumpy form, and the tsetse fly. Stumpy forms arise at the peak of each parasitaemia and are committed to differentiation to procyclic forms that inhabit the tsetse midgut. We have identified a protein tyrosine phosphatase (TbPTP1) that inhibits trypanosome differentiation. Consistent with a tyrosine phosphatase, recombinant TbPTP1 exhibits the anticipated substrate and inhibitor profile, and its activity is impaired by reversible oxidation. TbPTP1 inactivation in monomorphic bloodstream trypanosomes by RNA interference or pharmacological inhibition triggers spontaneous differentiation to procyclic forms in a subset of committed cells. Consistent with this observation, homogeneous populations of stumpy forms synchronously differentiate to procyclic forms when tyrosine phosphatase activity is inhibited. Our data invoke a new model for trypanosome development in which differentiation to procyclic forms is prevented in the bloodstream by tyrosine dephosphorylation. It may be possible to use PTP1B inhibitors to block trypanosomatid transmission.

scholarly journals Characterization of the Activation of Protein Tyrosine Phosphatase, Receptor-Type, Z Polypeptide 1 (PTPRZ1) by Hypoxia Inducible Factor-2 Alpha

PLoS ONE ◽  
2010 ◽  
Vol 5 (3) ◽  
pp. e9641 ◽  
Author(s):  
Victoria Wang ◽  
David A. Davis ◽  
Ravindra P. Veeranna ◽  
Muzammel Haque ◽  
Robert Yarchoan
Keyword(s):  
Protein Tyrosine Phosphatase ◽  
Tyrosine Phosphatase ◽  
Hypoxia Inducible Factor ◽  
Receptor Type ◽  
Protein Tyrosine
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