Evaluation of endogenous control genes for gene expression studies across multiple tissues and in the specific sets of fat- and muscle-type samples of the pig

2011 ◽  
Vol 128 (4) ◽  
pp. 319-325 ◽  
Author(s):  
Y.R. Gu ◽  
M.Z. Li ◽  
K. Zhang ◽  
L. Chen ◽  
A.A. Jiang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Endogenous Control ◽  
Muscle Type ◽  
Expression Studies ◽  
Gene Expression Studies

scholarly journals Evaluation of endogenous control gene(s) for gene expression studies in human blood exposed to 60 Co γ-rays ex vivo

2014 ◽  
Vol 56 (1) ◽  
pp. 177-185 ◽  
Author(s):  
S. Thangminlal Vaiphei ◽  
Joshua Keppen ◽  
Saibadaiahun Nongrum ◽  
R.C. Chaubey ◽  
L. Kma ◽  
...  
Keyword(s):  
Gene Expression ◽  
Human Blood ◽  
Ex Vivo ◽  
Endogenous Control ◽  
Control Gene ◽  
Endogenous Control Gene ◽  
Expression Studies ◽  
Γ Rays ◽  
Gene Expression Studies

scholarly journals Validation of endogenous control reference genes for normalizing gene expression studies in endometrial carcinoma

2015 ◽  
Vol 21 (9) ◽  
pp. 723-735 ◽  
Author(s):  
Thangesweran Ayakannu ◽  
Anthony H. Taylor ◽  
Jonathon M. Willets ◽  
Laurence Brown ◽  
David G. Lambert ◽  
...  
Keyword(s):  
Gene Expression ◽  
Endometrial Carcinoma ◽  
Reference Genes ◽  
Endogenous Control ◽  
Expression Studies ◽  
Gene Expression Studies

Identification of endogenous control genes for gene expression studies in peripheral blood of patients with coronary artery disease

2013 ◽  
Vol 47 (2) ◽  
pp. 192-196 ◽  
Author(s):  
Siew Wai Fong ◽  
Suhairi Ibrahim ◽  
Mohd Sapawi Mohamed ◽  
Ling Ling Few ◽  
Wei Cun See Too ◽  
...  
Keyword(s):  
Gene Expression ◽  
Coronary Artery Disease ◽  
Coronary Artery ◽  
Peripheral Blood ◽  
Endogenous Control ◽  
Expression Studies ◽  
Gene Expression Studies ◽  
Artery Disease

scholarly journals Assessment of common housekeeping genes as reference for gene expression studies using RT-qPCR in mouse choroid plexus

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kim Hoa Ho ◽  
Annarita Patrizi
Keyword(s):  
Gene Expression ◽  
Choroid Plexus ◽  
Sensory Deprivation ◽  
Housekeeping Genes ◽  
Housekeeping Gene ◽  
Experimental Conditions ◽  
Brain Repair ◽  
Expression Studies ◽  
Testing Algorithms ◽  
Gene Expression Studies

AbstractChoroid plexus (ChP), a vascularized secretory epithelium located in all brain ventricles, plays critical roles in development, homeostasis and brain repair. Reverse transcription quantitative real-time PCR (RT-qPCR) is a popular and useful technique for measuring gene expression changes and also widely used in ChP studies. However, the reliability of RT-qPCR data is strongly dependent on the choice of reference genes, which are supposed to be stable across all samples. In this study, we validated the expression of 12 well established housekeeping genes in ChP in 2 independent experimental paradigms by using popular stability testing algorithms: BestKeeper, DeltaCq, geNorm and NormFinder. Rer1 and Rpl13a were identified as the most stable genes throughout mouse ChP development, while Hprt1 and Rpl27 were the most stable genes across conditions in a mouse sensory deprivation experiment. In addition, Rpl13a, Rpl27 and Tbp were mutually among the top five most stable genes in both experiments. Normalisation of Ttr and Otx2 expression levels using different housekeeping gene combinations demonstrated the profound effect of reference gene choice on target gene expression. Our study emphasized the importance of validating and selecting stable housekeeping genes under specific experimental conditions.

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