Inheritance of Dry Root Rot (Rhizoctonia bataticola) Resistance in Chickpea (Cicer arietinum)

Background: The plant growth promotion and efficacy against phytopathogens by the endophytic bacteria are being focused now due to their ecofriendly nature. Methods: Endophytic bacteria (24 nos) isolated from the roots, stems and leaves of black gram plants collected from different locations were tested for their potential to inhibit the growth of R. bataticola under in vitro and in vivo conditions. Result: The leaf endophyte BLE 4 exhibited maximum inhibition (79.6%) of R. bataticola followed by BSE 4 (77.4%), BSE 7 (77.0%) and BLE 1 (74.0%). Among these 4 isolates tested as seed treatment and soil application, there was significant increase in dry weight (7.1 g), plant height (37.7 cm), number of branches (13.2) and number of pods (26.2) in BLE 4 treated plots. Whereas, the incidence of dry root rot and yield were insignificant. In the screening study of isolates for their phosphate solubilization potential, protease activity, siderophore and HCN production, no single isolate possessed all the properties, but siderophore production, protease activity and phosphate solubilization were found in BSE 4, BRE 3, BRE 5 and BRE 10 isolates.

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Rapid and sensitive diagnoses of dry root rot pathogen of chickpea (Rhizoctonia bataticola (Taub.) Butler) using loop-mediated isothermal amplification assay

Scientific Reports ◽

10.1038/srep42737 ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 10

Author(s):

Raju Ghosh ◽

Avijit Tarafdar ◽

Mamta Sharma

Keyword(s):

Root Rot ◽

Isothermal Amplification ◽

Rhizoctonia Bataticola ◽

Loop Mediated Isothermal Amplification ◽

Amplification Assay

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Utilizing the combined antifungal potential of Trichoderma spp. and organic amendments against dry root rot of mungbean

Egyptian Journal of Biological Pest Control ◽

10.1186/s41938-019-0187-8 ◽

2019 ◽

Vol 29 (1) ◽

Cited By ~ 1

Author(s):

Anam Choudhary ◽

Shabbir Ashraf

Keyword(s):

Root Rot ◽

Root Nodules ◽

Organic Amendments ◽

Dry Weight ◽

Dual Culture ◽

Trichoderma Spp ◽

Rhizoctonia Bataticola ◽

Neem Cake ◽

Greenhouse Study

AbstractThe present study was carried out to evaluate the effect of bioagents and organic amendments in suppressing the dry root rot of mungbean incited by Rhizoctonia bataticola. The locally isolated pathogen and fungal biocontrol agents were identified based on morphological and molecular characterization. These identified bioagents were tested in vitro, and the highest mycelial inhibition was recorded in dual culture assay by Trichoderma harzianum (74.44%), and among organic amendments, maximum mycelial inhibition was found in neem cake (61.11%). In a greenhouse study, T. harzianum + neem cake effectively enhanced the percent germination (93.33%) and decreased the percent disease mortality (11.67%) than the other treatments. The morphological parameter like plant height (57.50 cm), dry weight (22.83 g) root nodules (51), pods/plant (58), and 100-seed weight (5.78 g) were found to be at the maximum in this combined application. Physiological pigments viz. chlorophyll (2.41 mg/g) and carotenoids (0.19 mg/g), protein content (5.85 mg/g), and leghemoglobin (11.75 mg/g) were also found to be maximum in T. harzianum + neem cake and minimum phenol content (1.41 mg/g). The study concludes that T. harzianum + neem cake can be recommended as an effective approach for the management of dry root rot of mungbean.

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