Identification and characterization of genomic DNA sequences of the S-ribonuclease gene associated with self-incompatibility alleles S1to S5in European pear

Abstract In Brassica species that exhibit self-incompatibility, two genes, SLG and SRK, at the S locus are involved in the recognition reaction with self and non-self pollen. From a pollen-recessive S29 haplotype of Brassica rapa, both cDNA and genomic DNA clones for these two genes were isolated and characterized. The nucleotide sequence for the S domain of SRK29 showed a high degree of similarity with that of SLG29, and they belong to Class II type. RNA gel blot analysis showed that the transcript of SLG29 consisted of the first and second exons, and no other transcript containing any part of the intron sequence was detected. Because no transmembrane domain was encoded by the second exon of SLG29, SLG29 was designated a secreted type glycoprotein. SLGs of two other pollen-recessive haplotypes, S40 and S44, of B. rapa also had a similar structure to that of SLG29. Previously, SLG2 from a pollen-recessive haplotype, S2, of Brassica oleracea was found to produce two different transcripts, one for the secreted type glycoprotein and the other for a putative membrane-anchored form of SLG. Therefore, the nature of these SLGs from pollen-recessive haplotypes of B. rapa is different from that of SLG2 of B. oleracea.

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Survey, Identification, and Characterization of Cylindrocarpon-Like Asexual Morphs in Spanish Forest Nurseries

Plant Disease ◽

10.1094/pdis-01-18-0171-re ◽

2018 ◽

Vol 102 (11) ◽

pp. 2083-2100 ◽

Cited By ~ 4

Author(s):

Beatriz Mora-Sala ◽

Ana Cabral ◽

Maela León ◽

Carlos Agustí-Brisach ◽

Josep Armengol ◽

...

Keyword(s):

Dna Sequences ◽

Elongation Factor ◽

Its Region ◽

Phylogenetic Position ◽

Translation Elongation ◽

Forest Nurseries ◽

Asexual Morphs ◽

Identification And Characterization ◽

Elongation Factor 1

Cylindrocarpon-like asexual morphs infect herbaceous and woody plants, mainly in agricultural scenarios, but also in forestry systems. The aim of the present study was to characterize a collection of Cylindrocarpon-like isolates recovered from the roots of a broad range of forest hosts from nurseries showing decline by morphological and molecular studies. Between 2009 and 2012, 17 forest nurseries in Spain were surveyed and a total of 103 Cylindrocarpon-like isolates were obtained. Isolates were identified based on DNA sequences of the partial gene regions histone H3 (his3). For the new species, the internal transcribed spacer and intervening 5.8S nrRNA gene (ITS) region, β-tubulin (tub2), and translation elongation factor 1-α (tef1) were also used to determine their phylogenetic position. Twelve species belonging to the genera Cylindrodendrum, Dactylonectria, and Ilyonectria were identified from damaged roots of 15 different host genera. The species C. alicantinum, D. macrodidyma, D. novozelandica, D. pauciseptata, D. pinicola, D. torresensis, I. capensis, I. cyclaminicola, I. liriodendri, I. pseudodestructans, I. robusta, and I. rufa were identified. In addition, two Dactylonectria species (D. hispanica sp. nov. and D. valentina sp. nov.), one Ilyonectria species (I. ilicicola sp. nov.), and one Neonectria species (N. quercicola sp. nov.) are newly described. The present study demonstrates the prevalence of this fungal group associated with seedlings of diverse hosts showing decline symptoms in forest nurseries in Spain.

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Identification and Characterization of Components of a Putative Petunia S-Locus F-Box–Containing E3 Ligase Complex Involved in S-RNase–Based Self-Incompatibility

The Plant Cell ◽

10.1105/tpc.106.041061 ◽

2006 ◽

Vol 18 (10) ◽

pp. 2531-2553 ◽

Cited By ~ 115

Author(s):

Zhihua Hua ◽

Teh-hui Kao

Keyword(s):

E3 Ligase ◽

Self Incompatibility ◽

S Locus ◽

Identification And Characterization

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Characterization of equine cDNA sequences for αS1-, β- and κ-casein

Journal of Dairy Research ◽

10.1017/s002202990200599x ◽

2003 ◽

Vol 70 (1) ◽

pp. 29-36 ◽

Cited By ~ 23

Author(s):

Tina Lenasi ◽

Irena Rogelj ◽

Peter Dovc

Keyword(s):

Dna Sequences ◽

Genomic Dna ◽

Genomic Region ◽

Interspecies Comparison ◽

Cryptic Exon ◽

Conserved Sequence ◽

Cdna Sequences ◽

Sequence Identity ◽

Lactating Mammary Gland

Here we report the entire cDNA sequences for equine αS1-, β- and κ-casein. Based on interspecies comparison, nine exons were found in equine β-casein and five in κ-casein. In equine αS1-casein cDNA the exon 5 was missing, which resulted in the total of 18 exons instead of 19 theoretically possible exons in αS1-casein cDNA. Comparison of DNA sequences representing exon 5 in other species with corresponding equine genomic region confirmed the presence of cryptic exon in horse genomic DNA. Equine αS1-casein mRNA was present in three forms in the lactating mammary gland and we showed that the two shorter forms were produced by skipping either the exon 8 or exon 15. In horse, as in some other mammals, β- and κ-casein are considerably more conserved (sequence identity 53% to 59% and 57% to 67%, respectively) than αS1-casein which appears as the most variable casein among species (sequence identity 40% to 54%). Interestingly, horse caseins resemble human much more than bovine caseins which may also explain the high dietetic value of mares' milk.

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Cloning and characterization of an alternative splicing transcript of the gene coding for human cytidine deaminase

Biochemistry and Cell Biology ◽

10.1139/o06-197 ◽

2007 ◽

Vol 85 (1) ◽

pp. 96-102 ◽

Cited By ~ 1

Author(s):

Bianca Cristina Garcia Lisboa ◽

Tamara da Rocha Machado ◽

Daniel Carvalho Pimenta ◽

Sang Won Han

Keyword(s):

Alternative Splicing ◽

Dna Sequences ◽

Genomic Sequence ◽

Cytidine Deaminase ◽

Alternative Form ◽

Nih3t3 Cells ◽

Reading Frame ◽

Gene Coding ◽

Identification And Characterization

Human cytidine deaminase (HCD) catalyzes the deamination of cytidine or deoxycytidine to uridine or deoxyuridine, respectively. The genomic sequence of HCD is formed by 31 kb with 4 exons and several alternative splicing signals, but an alternative form of HCD has yet to be reported. Here we describe the cloning and characterization of a small form of HCD, HSCD, and it is likely to be a product of alternative splicing of HCD. The alignment of DNA sequences shows that the HSCD matches HCD in 2 parts, except for a deletion of 170 bp. Based on the HCD genome organization, exons 1 and 4 should be joined and all sequences of introns and exons 2 and 3 should be deleted by splicing. This alternative splicing shifted the translation of the reading frame from the point of splicing. The estimated molecular mass is 9.8 kDa, and this value was confirmed by Western blot and mass spectroscopy after expressing the gene fused with glutathionine-S-transferase in the pGEX vector. The deletion and shift of the reading frame caused a loss of HCD activity, which was confirmed by enzyme assay and also with NIH3T3 cells modified to express HSCD and challenged against cytosine arabinoside. In this work we describe the identification and characterization of HSCD, which is the product of alternative splicing of the HCD gene.

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