scholarly journals Intracellular Localization of DNA Polymerases in the Oocyte of Starfish, Asterina pectinifera. (starfish/DNA polymerase alpha/DNA polymerase beta/germinal vesicle/enucleation)

1983 ◽  
Vol 25 (6) ◽  
pp. 547-551 ◽  
Author(s):  
NOBORU OISHI ◽  
HIRAKU SHIMADA
Keyword(s):  
Dna Polymerase ◽  
Intracellular Localization ◽  
Dna Polymerases ◽  
Germinal Vesicle ◽  
Dna Polymerase Beta ◽  
Dna Polymerase Alpha ◽  
Polymerase Beta ◽  
Asterina Pectinifera

scholarly journals Involvement of deoxyribonucleic acid polymerase β in nuclear deoxyribonucleic acid synthesis

1978 ◽  
Vol 173 (1) ◽  
pp. 309-314 ◽  
Author(s):  
T R Butt ◽  
W M Wood ◽  
E L McKay ◽  
R L P Adams
Keyword(s):  
Dna Synthesis ◽  
Dna Polymerase ◽  
Deoxyribonucleic Acid ◽  
Nuclear Dna ◽  
Dna Polymerase Beta ◽  
Cell Nuclei ◽  
High Molecular Weight Dna ◽  
Dna Polymerase Alpha ◽  
Polymerase Beta

The effects on DNA synthesis in vitro in mouse L929-cell nuclei of differential extraction of DNA polymerases alpha and beta were studied. Removal of all measurable DNA polymerase alpha and 20% of DNA polymerase beta leads to a 40% fall in the replicative DNA synthesis. Removal of 70% of DNA polymerase beta inhibits replicative synthesis by 80%. In all cases the nuclear DNA synthesis is sensitive to N-ethylmaleimide and aCTP (arabinosylcytosine triphosphate), though less so than DNA polymerase alpha. Addition of deoxyribonuclease I to the nuclear incubation leads to synthesis of high-molecular-weight DNA in a repair reaction. This occurs equally in nuclei from non-growing or S-phase cells. The former nuclei lack DNA polymerase alpha and the reaction reflects the sensitivity of DNA polymerase beta to inhibiton by N-ethylmaleimide and aCTP.

scholarly journals Purification, biochemical characterization and serological analysis of cellular deoxyribonucleic acid polymerases and a reverse transcriptase from spleen of a patient with myelofibrotic syndrome

1977 ◽  
Vol 167 (3) ◽  
pp. 513-524 ◽  
Author(s):  
P Chandra ◽  
L K Steel
Keyword(s):  
Reverse Transcriptase ◽  
Dna Polymerase ◽  
Dna Polymerase Beta ◽  
Human Spleen ◽  
Leukaemia Virus ◽  
Polymerase Gamma ◽  
Dna Polymerase Alpha ◽  
Polymerase Beta ◽  
Dna Polymerase Gamma ◽  
Cellular Dna

The present study describes the separation and purification of a reverse transcriptase and cellular DNA polymerases from the human spleen of a patient with myelofibrotic syndrome. The specific requirements with respect to bivalent cations and template-primers for DNA polymerase-alpha, DNA polymerase-beta and DNA polymerase-gamma, as well as for the reverse transcriptase, are reported. Sedimentation-velocity measurements of the purified enzymes gave values of 150000, 40000, 100000 and 70000 daltons for DNA polymerase-alpha DNA polymerase-beta, DNA polymerase-gamma and the reverse transcriptase respectively. Serological studies have shown that the reverse transcriptase from human spleen is not antigenically related to cellular DNA polymerase-alpha, -beta or -gamma, but is antigenically related to reverse transcriptase from simian sarcoma virus and gibbon-ape leukaemia virus.

scholarly journals Chick embryo DNA polymerase beta. Purified enzyme consists of a single Mr = 40,000 polypeptide.

1980 ◽  
Vol 255 (20) ◽  
pp. 9942-9948 ◽  
Author(s):  
M. Yamaguchi ◽  
K. Tanabe ◽  
Y.N. Taguchi ◽  
M. Nishizawa ◽  
T. Takahashi ◽  
...  
Keyword(s):  
Chick Embryo ◽  
Dna Polymerase ◽  
Dna Polymerase Beta ◽  
Polymerase Beta
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