PREPUBERTAL GYNAECOMASTIA IN ASSOCIATION WITH AN INTERSTITIAL-CELL TUMOUR OF THE TESTIS

SUMMARY Steroid metabolism by a unique, transplantable, oestrogen-induced, hormone-dependent interstitial-cell tumour of a rat testis was investigated. After incubation of the minced tumour in medium without substrate the presence of 3α-androstanediol, 3-epiandrosterone and androsterone was shown. There was inconclusive evidence for the presence of smaller amounts of 3β-androstanediol, and no evidence for testosterone. [14C]Pregnenolone substrate was shown to be metabolized after 2 h to androstenedione, androsterone and a small amount of testosterone. Epi- and dehydroepiandrosterone were probably formed from [14C]pregnenolone, but were incompletely characterized.

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A COMPARATIVE STUDY OF THE BIOSYNTHESIS OF TESTOSTERONE BY HUMAN TESTES AND A VIRILIZING INTERSTITIAL CELL TUMOUR

Acta Endocrinologica ◽

10.1530/acta.0.0560726 ◽

1967 ◽

Vol 56 (4) ◽

pp. 726-736 ◽

Cited By ~ 8

Author(s):

D. C. Sharma ◽

E. A. Racz ◽

R. I. Dorfman ◽

E. J. Schoen

Keyword(s):

Interstitial Cell ◽

Testicular Tissue ◽

Cell Tumour ◽

Isotopic Dilution ◽

Testicular Tumour ◽

Early Puberty ◽

Laboratory Findings ◽

Major Reaction ◽

The Right

ABSTRACT A comparative in vitro study of an interstitial cell tumour of the testis from a virilized patient and »normal« testes from another male has been made by incubating homogenates of these tissues with radioactive progesterone, 17α-hydroxyprogesterone, testosterone and androst-4-ene-3,17-dione-4-14C. The patient with the testicular tumour was a 14-year old boy whose clinical studies, reported in detail elsewhere, revealed a history of early puberty and laboratory findings of markedly elevated levels of urinary total 17-ketosteroids and pregnanetriol. These urinary values fell to normal following removal of the right testicular tumour, which was interpreted as being a benign interstitial cell tumour on histologic examination. The radioactive metabolites from the testicular tissue of both subjects were separated, purified and identified by partition column chromatography in various systems with authentic standards, formation of derivatives and isotopic dilution analysis. The interstitial cell tumour converted a significant amount of the substrate progesterone into 16α-hydroyprogesterone, besides testosterone and androst-4-ene-3,17-dione; reduction at C20 was also a major reaction when either progesterone or 17α-hydroxyprogesterone was the substrate. On a comparative basis the activity of C17-20 desmolase was about five times more in the tumour as compared to the testicular tissue.

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Neoplasia in the Siberian weasel (Mustela sibirica): two case reports of fibrosarcoma and interstitial cell tumour

European Journal of Wildlife Research ◽

10.1007/s10344-007-0101-7 ◽

2007 ◽

Vol 54 (1) ◽

pp. 15-20 ◽

Cited By ~ 3

Author(s):

Martina Zöller ◽

Famke Aeffner ◽

Jochen Müller ◽

Kerstin Mätz-Rensing ◽

Franz-Josef Kaup

Keyword(s):

Interstitial Cell ◽

Case Reports ◽

Cell Tumour

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MASCULINIZING EFFECTS OF A TESTICULAR INTERSTITIAL CELL TUMOUR GRAFT ON FEMALE RATS

Journal of Endocrinology ◽

10.1677/joe.0.0590353 ◽

1973 ◽

Vol 59 (2) ◽

pp. 353-361

Author(s):

NATALIE POURREAU-SCHNEIDER

Keyword(s):

Interstitial Cell ◽

Mating Behaviour ◽

Reproductive Function ◽

Cell Tumour ◽

Female Rats ◽

Surgical Removal ◽

Follicular Atresia ◽

Hormone Production ◽

Target Organs ◽

Palpable Nodule

SUMMARY An androgen-producing testicular interstitial cell tumour of rats was grafted into intact and spayed female rats. The tumour inhibited luteinization, produced follicular atresia, stimulated the uterine myometrium and endometrium, and caused vaginal mucus formation. Anoestrus set in and mating behaviour disappeared, rendering gestation impossible. After surgical removal of the tumour, the masculinization disappeared rapidly. With the return of vaginal oestrus (sometimes only 4 days after removal of the tumour) most of the rats mated and gave birth to normal young. When a palpable nodule reappeared, the reproductive function was again lost. The tumour produced similar changes in the target organs of spayed females. The inhibitory action of the endocrine tumour on pituitary gonadotrophic hormone production was shown by the absence of castration cells in the pituitary of the tumour-bearing spayed female.

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STEROID METABOLISM IN VITRO BY AN INTERSTITIAL CELL TUMOUR AND THE ATTACHED PREPUBERTAL TESTIS

Journal of Endocrinology ◽

10.1677/joe.0.0590637 ◽

1973 ◽

Vol 59 (3) ◽

pp. 637-649 ◽

Cited By ~ 3

Author(s):

GWEN RICHARDS ◽

A. MUNRO NEVILLE

Keyword(s):

Metabolic Pathways ◽

Interstitial Cell ◽

Reductase Activity ◽

Testicular Tissue ◽

Cell Tumour ◽

Tumour Tissue ◽

Steroid Metabolism

SUMMARY A virilizing interstitial cell tumour and the attached testicular tissue from a 4-year-old boy were incubated in vitro with [7α-3H]pregnenolone and [4-14C]progesterone, or [4-14C]androstenedione and [7α-3H]5α-dihydrotestosterone. Ring A saturated steroids were produced from 4-ene precursors by the prepubertal testis, but this tissue was unable to convert pregnenolone or progesterone to 17α-hydroxylated C21 steroids, or to C19 steroids. The virilizing interstitial cell tumour metabolized pregnenolone and progesterone to 17α-hydroxyprogesterone, androstenedione and testosterone. In addition, dehydroepiandrosterone was detected as a product of pregnenolone. The tumour lacked 4-ene-5α-steroid reductase activity. 5α-Dihydrotestosterone was metabolized to 5α-androstane-3,17-dione, androsterone, isoandrosterone, 5a-androstane-3α,17β-diol and 5α-androstane-3β,17β-diol in both the normal and tumour tissue. The significance of these metabolic pathways is discussed.

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Contrast-enhanced ultrasound evaluation of testicular interstitial cell tumours in conscious non-sedated dogs

Veterinární Medicína ◽

10.17221/101/2017-vetmed ◽

2018 ◽

Vol 63 (No. 3) ◽

pp. 125-130 ◽

Cited By ~ 1

Author(s):

M. Quartuccio ◽

C. Mangano ◽

F. Macri ◽

M. Rizzo ◽

S. Di Pietro ◽

...

Keyword(s):

Interstitial Cell ◽

Regional Blood Flow ◽

Mean Transit Time ◽

Cell Tumour ◽

Surrounding Tissue ◽

Histological Evaluation ◽

Single Type ◽

Contrast Enhanced Ultrasound ◽

Ultrasound Evaluation ◽

Contrast Enhanced

Testicular tumours are the most common neoplasms of the genital system in male dogs. The three main types reported in dogs are interstitial cell tumour, seminoma and Sertoli cell tumour. Interstitial cell tumour is related to the presence of single or multiple nodules inside the testicular parenchyma, and it is detected by palpation or is often an incidental finding during ultrasonography examination. Contrast-enhanced ultrasound allows characterisation of the perfusion of the testicular lesion and reveals the micro-vascularisation; however, perfusion parameters may be strongly influenced by sedative drug administration, so our aim was to evaluate qualitative and quantitative perfusion of a single type of tumour (interstitial cell tumour) with contrast-enhanced ultrasound in conscious dogs to exclude any influence of pharmacological agents on vascular flow. Thirty dogs with focal testicular lesions found by palpation and/or by ultrasound (B-mode and Doppler) examination were selected; contrast-enhanced ultrasound was performed only in subjects that presented testicular focal lesions. After orchiectomy, testes were submitted to histological evaluation; 2-minute clips recorded during contrast-enhanced ultrasound were analysed only in the case of dogs with interstitial cell tumours (n = 12). Contrast medium showed wash-in at around 25–30 seconds, at the same time as the surrounding tissue: lesions were hyperenhancing, homogeneous or inhomogeneous with rim enhancement and contained prominent inner vessels; however, enhancement of small regions was absent. Quantitative analysis demonstrated significantly higher PI% (P = 0.005), regional blood volume (P = 0.02) and regional blood flow (P = 0.007) values in lesions than in surrounding tissue; no differences were found for time-to-peak and mean transit time. In conclusion, the contrast-enhanced ultrasound pattern observed in conscious non-sedated dogs with interstitial cell tumour was similar to the pattern described in a previous study in dogs after intramuscular administration of medetomidine (10 µg/kg) and butorphanol (0.2 mg/kg).

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