Occurrence and relevance of Mycoplasma spp. in free-ranging pheasants from northwestern Germany

Since 2008/2009, the population of free-ranging ring-necked pheasants was recorded to decrease all over Germany. Various Mycoplasma (M.) spp. are causing severe respiratory signs in captive game bird species. Furthermore, M. gallisepticum is responsible for massive die-offs in consequence to severe conjunctivitis in house finches in the USA. Therefore, the prevalence of mycoplasmas in free-ranging pheasants was investigated and a potential impact on the population decline of pheasants discussed. Within this study, 150 free-ranging pheasants were sampled via tracheal swabs and tissue samples of the trachea and the periorbital skin, as the latter displayed inflammatory alterations in previous studies. In total, 177 samples were investigated for the presence of mycoplasmas using cultural and molecular biological methods. In 76 birds, necropsy was performed additionally. In total, 73.7% (51/76) of the examined pheasants had periorbital skin alterations. Furthermore, 64.4% (114/177) of the samples tested positive for mycoplasmas via PCR. Overall, 102/177 samples (57.6%, 78/105 tracheal swabs, 19/51 skin tissue, 5/21 trachea tissue) tested positive for mycoplasmas via culture. Mycoplasma gallinaceum (n = 50), M. pullorum (n = 45), M. glycophilum (n = 43), M. iners (n = 11), and M. gallinarum (n = 5) were frequently isolated. In 45 cases (45.9%), multiple Mycoplasma spp. were isolated from one sample. All examined samples tested negative for M. gallisepticum. Of 51 skin samples investigated for mycoplasmas, 24 (47.1%) showed inflammatory skin alterations in histology, and 58.3% (14/24) of these samples tested positive for Mycoplasma spp. additionally. Overall, there was a significant correlation between inflammatory altered skin samples and the detection of mycoplasmas in periorbital skin samples. Based on the present results, the isolated Mycoplasma spp. may play a role as facultative agents for the observed inflammatory skin alterations. However, additional investigation is needed to confirm this presumption.

Absence of Mycoplasma spp. in nightingales (Luscinia megarhynchos) and blue (Cyanistes caeruleus) and great tits (Parus major) in Germany and its potential implication for evolutionary studies in birds

Mycoplasma spp. are important pathogens in poultry and cause high economic losses for poultry industry worldwide. In other bird species (e.g. white storks, birds of prey, and several waterfowl species), Mycoplasma spp. are regularly found in healthy individuals, hence, considered apathogenic or part of the microbiota of the upper respiratory tract. However, as Mycoplasma spp. are absent in healthy individuals of some wild bird species, they might play a role as respiratory pathogen in these bird species, e.g. Mycoplasma gallisepticum in house finches. The knowledge on the occurrence of Mycoplasma spp. in wild birds is limited. To evaluate the relevance of Mycoplasma spp. in free-ranging nightingales and tits, 172 wild caught birds were screened for the presence of mycoplasmas. The birds were sampled via choanal swabs and examined via molecular methods (n = 172) and, when possible, via culture (n = 142). The Mycoplasma sp. was determined by sequencing the 16S rRNA gene and 16S-23S Intergenic Transcribed Spacer Region. All birds were tested negative for mycoplasmas via PCR and/or mycoplasmal culture. Hence, free-ranging nightingales and tits do not show any mycoplasma in their microbial flora of the respiratory tract. Therefore, these songbird species may suffer from clinical mycoplasmosis when being infected. We hypothesize that birds relying on their vocal ability for reproduction have excluded mycoplasmas from their respiratory flora compared to other bird species.

Renewed coexistence: learning from steering group stakeholders on a beaver reintroduction project in England

Species reintroductions are growing in popularity, and example motivations include supporting species populations or the restoration of ecosystem function. Interactions between humans and the reintroduced species are likely to occur post-reintroduction. Coexistence between humans and wildlife is adaptive and dynamic, in part requiring management of conflicts between humans and wildlife, or of conflicts between humans over wildlife management. We seek to learn from the experiences of steering group members in a Eurasian beaver (Castor fiber) project in England and identify how governance of coexistence with reintroduced species may differ from the governance of coexistence with species that are already present in the landscape. Using a qualitative thematic analysis of an online survey, we identify a series of lessons in six key areas: (1) project governance, (2) stakeholder engagement, (3) research and monitoring programme, (4) strategy to manage arising conflicts, (5) public engagement, and (6) broad perspectives on reintroduction trials. We advocate for reflective evaluation as an essential component of reintroduction projects to enable knowledge-sharing from experiences, leading to improved practices in the future. Reflecting on our analysis, we identify and define ‘Renewed Coexistence’—a new term that draws on pre-existing coexistence knowledge but identifies the unique elements that relate to governing coexistence with reintroduced species.

The faded stripes of Bengal: a historical perspective on the easternmost distribution of the striped hyena

The striped hyena (Hyaena hyaena) is an understudied large carnivore with no known historic range map. Knowledge of the past and present extent of its easternmost distribution beyond 85° east longitude is dubious. Through a comprehensive review of historical evidence and contemporary records, we investigated striped hyena presence in Bengal, i.e., Bangladesh and the Indian state of West Bengal in South Asia. We found 14 historical records, with the oldest one dating to 1876. Our review establishes Bangladesh as a former striped hyena range country and the striped hyena as one of the first large carnivores to go extinct in Bangladesh. We identified northern Bangladesh as part of its historical range (until ~1965), and south-central Bangladesh as a possible part of its historical range. In West Bengal, India, hyenas were historically present up to the southern tributaries of the Brahmaputra River, but the present range is reduced. The area south of the Damodar River is its last refuge in Bengal. We also found 15 contemporary records (2010–2021) in Bengal, from sites situated on the eastern limit of the Chota Nagpur Plateau. These records noted 25 sightings including 9 deaths due to poaching, train accidents, and retaliatory killings. Our review demonstrates that hyenas are currently present up to 87° east longitude, which extends the currently documented easternmost range for the species by almost 1,000 km. We recommend methods which can be applied to delineate the historical extent of striped hyenas elsewhere as well as for other poorly understood species.