CERAMIDE-GALACTOSYLTRANSFERASE AND CEREBROSIDE-SULPHOTRANSFERASE LOCALISATION IN GOLGI MEMBRANES ISOLATED BY A CONTINUOUS SUCROSE GRADIENT OF MOUSE BRAIN MICROSOMES

1979 ◽  
Vol 33 (2) ◽  
pp. 497-504 ◽  
Author(s):  
H. P. Siegrist ◽  
T. Burkart ◽  
U. N. Wiesmann ◽  
N. N. Herschkowitz ◽  
M. A. Spycher
Keyword(s):  
Mouse Brain ◽  
Sucrose Gradient ◽  
Golgi Membranes ◽  
Continuous Sucrose Gradient ◽  
Brain Microsomes

LOCALIZATION OF 2',3'-CYCLIC NUCLEOTIDE-3'-PHOSPHOHYDROLASE OF RABBIT BRAIN BY SEDIMENTATION IN A CONTINUOUS SUCROSE GRADIENT

1978 ◽  
Vol 30 (4) ◽  
pp. 735-744 ◽  
Author(s):  
R. Shapira ◽  
W. C. Mobley ◽  
S. B. Thiele ◽  
M. R. Wilhelmi ◽  
A. Wallace ◽  
...  
Keyword(s):  
Cyclic Nucleotide ◽  
Sucrose Gradient ◽  
Rabbit Brain ◽  
Continuous Sucrose Gradient

scholarly journals High activity of cytochrome P-450-linked aminopyrine N-demethylase in mouse brain microsomes, and associated sex-related difference

1989 ◽  
Vol 261 (3) ◽  
pp. 769-773 ◽  
Author(s):  
V Ravindranath ◽  
H K Ananda Theertha Varada
Keyword(s):  
Mouse Brain ◽  
Male Mouse ◽  
Mouse Liver ◽  
Double Diffusion ◽  
Microsomal Protein ◽  
Cross Reactivity ◽  
Related Difference ◽  
Brain Microsomes ◽  
Demethylase Activity ◽  
Cytochrome P 450

The presence of cytochrome P-450 and associated mono-oxygenase activities was examined in brain microsomes from male and female mice. Although the cytochrome P-450 level in male mouse brain was very low as compared with mouse liver, the aminopyrine N-demethylase and morphine N-demethylase specific activities in male mouse brain were much higher than those observed in mouse liver. Ethoxycoumarin O-de-ethylase and aniline hydroxylase activities were, however, not detected in mouse brain. Sex-related differences were observed in both the cytochrome P-450 levels and aminopyrine N-demethylase activity in mouse brain, the levels of both being higher in male mouse brain as compared with female mouse brain. Aminopyrine N-demethylase activity in mouse brain microsomes was dependent on the presence of oxygen and NADPH and could be inhibited by piperonyl butoxide, N-octyl imidazole and carbon monoxide. Antiserum raised to the phenobarbital-inducible form of rat liver cytochrome P-450 [P-450(b+e)] inhibited mouse brain aminopyrine N-demethylase activity by around 80+ mouse brain microsomal protein exhibited cross-reactivity against this antiserum when examined by Ouchterlony double diffusion and immunoblotting. The present results indicate the presence of a phenobarbital-inducible form of cytochrome P-450 (or a form of cytochrome P-450 that is similar immunologically) in mouse brain microsomes, which is associated with a sex-related difference.

Possible involvement of Cyp3a enzymes in the metabolism of tetrahydrocannabinols by mouse brain microsomes

2010 ◽  
Vol 29 (1) ◽  
pp. 56-60 ◽  
Author(s):  
Kazuhito Watanabe ◽  
Mai Fujinami ◽  
Satoshi Yamaori ◽  
Ikuo Yamamoto
Keyword(s):  
Mouse Brain ◽  
Brain Microsomes
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