The State of Accumulated Calcium in Mouse Brain Microsomes In Vitro1

1979 ◽  
Vol 85 (6) ◽  
pp. 1439-1446
Author(s):  
Daisaku SATOMI
Keyword(s):  
Mouse Brain ◽  
The State ◽  
Brain Microsomes

scholarly journals High activity of cytochrome P-450-linked aminopyrine N-demethylase in mouse brain microsomes, and associated sex-related difference

1989 ◽  
Vol 261 (3) ◽  
pp. 769-773 ◽  
Author(s):  
V Ravindranath ◽  
H K Ananda Theertha Varada
Keyword(s):  
Mouse Brain ◽  
Male Mouse ◽  
Mouse Liver ◽  
Double Diffusion ◽  
Microsomal Protein ◽  
Cross Reactivity ◽  
Related Difference ◽  
Brain Microsomes ◽  
Demethylase Activity ◽  
Cytochrome P 450

The presence of cytochrome P-450 and associated mono-oxygenase activities was examined in brain microsomes from male and female mice. Although the cytochrome P-450 level in male mouse brain was very low as compared with mouse liver, the aminopyrine N-demethylase and morphine N-demethylase specific activities in male mouse brain were much higher than those observed in mouse liver. Ethoxycoumarin O-de-ethylase and aniline hydroxylase activities were, however, not detected in mouse brain. Sex-related differences were observed in both the cytochrome P-450 levels and aminopyrine N-demethylase activity in mouse brain, the levels of both being higher in male mouse brain as compared with female mouse brain. Aminopyrine N-demethylase activity in mouse brain microsomes was dependent on the presence of oxygen and NADPH and could be inhibited by piperonyl butoxide, N-octyl imidazole and carbon monoxide. Antiserum raised to the phenobarbital-inducible form of rat liver cytochrome P-450 [P-450(b+e)] inhibited mouse brain aminopyrine N-demethylase activity by around 80+ mouse brain microsomal protein exhibited cross-reactivity against this antiserum when examined by Ouchterlony double diffusion and immunoblotting. The present results indicate the presence of a phenobarbital-inducible form of cytochrome P-450 (or a form of cytochrome P-450 that is similar immunologically) in mouse brain microsomes, which is associated with a sex-related difference.

Possible involvement of Cyp3a enzymes in the metabolism of tetrahydrocannabinols by mouse brain microsomes

2010 ◽  
Vol 29 (1) ◽  
pp. 56-60 ◽  
Author(s):  
Kazuhito Watanabe ◽  
Mai Fujinami ◽  
Satoshi Yamaori ◽  
Ikuo Yamamoto
Keyword(s):  
Mouse Brain ◽  
Brain Microsomes

scholarly journals Biosynthesis in vitro of sialosylgalactosyldiacylglycerol by mouse brain microsomes.

1981 ◽  
Vol 256 (23) ◽  
pp. 12306-12309
Author(s):  
J. Pieringer ◽  
S. Keech ◽  
R.A. Pieringer
Keyword(s):  
Mouse Brain ◽  
Brain Microsomes

scholarly journals Studies on human anti-rabies immunization in Brazil: I - Evaluation of the 3 + 1 pre-exposure vaccination schedule under field conditions

1995 ◽  
Vol 37 (4) ◽  
pp. 349-352 ◽  
Author(s):  
Carlos Roberto Zanetti ◽  
Luciana Botelho Chaves ◽  
Andréa de Cássia Rodrigues Silva ◽  
Luiza Marny Lee ◽  
Octavio Augusto de Carvalho Pereira
Keyword(s):  
Mouse Brain ◽  
Vaccination Schedule ◽  
The State ◽  
Field Conditions ◽  
Routine Practice ◽  
Immunization Schedule ◽  
Suckling Mouse ◽  
Serum Samples ◽  
Local Conditions ◽  
The One

The currently used pre-exposure anti-rabies immunization schedule in Brazil is the one called 3+1, employing suckling mouse brain vaccine (3 doses on alternate days and the last one on day 30). Although satisfactory results were obtained in well controlled experimental groups using this immunization schedule, in our routine practice, VNA levels lower than 0.5 IU/ml are frequently found. We studied the pre-exposure 3+1 schedule under field conditions in different cities on the State of São Paulo, Brazil, under variable and sometimes adverse circumstances, such as the use of different batches of vaccine with different titers, delivered, stored and administered under local conditions. Fifty out of 256 serum samples (19.5%) showed VNA titers lower than 0.5 IU/ml, but they were not distributed homogeneously among the localities studied. While in some cities the results were completely satisfactory, in others almost 40% did not attain the minimum VNA titer required. The results presented here, considered separately, question our currently used procedures for human pre-exposure anti-rabies immunization. The reasons determining this situation are discussed.

Sign in / Sign up

Export Citation Format

Share Document