scholarly journals Molecular characterization of Serratia marcescens strains by RFLP and sequencing of PCR-amplified 16S rDNA and 16S?23S rDNA intergenic spacer

2007 ◽  
Vol 45 (2) ◽  
pp. 174-178 ◽  
Author(s):  
H. Zhu ◽  
W.Y. Zhou ◽  
M. Xu ◽  
Y.L. Shen ◽  
D.Z. Wei
Keyword(s):  
Serratia Marcescens ◽  
16S Rdna ◽  
Molecular Characterization ◽  
Intergenic Spacer ◽  
Rdna Intergenic Spacer

Molecular characterization of Candidatus Phytoplasma aurantifolia isolate infecting chickpea (Cicer arietinum) in Dharwad, Karnataka

2019 ◽  
Author(s):  
Gurupada Balol ◽  
C Channakeshava ◽  
M S Patil
Keyword(s):  
16S Rdna ◽  
Molecular Characterization ◽  
Cicer Arietinum ◽  
Nested Pcr ◽  
Rdna Sequences ◽  
Pcr Product ◽  
16S Rdna Sequences ◽  
Axillary Proliferation ◽  
Pale Green

Chickpea plants showing phytoplasma symptoms were observed in the research plots at University of Agricultural Sciences, Dharwad, Karnataka, India. The symptoms included phyllody, pale green leaves, bushy appearance and excessive axillary proliferation. The causal agent of the phyllody disease was identified based on symptoms, amplification of 16S rDNA of the phytoplasma by nested PCR with primers P1/P7 and R16F2n/R16R2 and 1,800 bp and 1,200 bp size products were amplified in first round PCR and nested-PCR respectively. The PCR product was sequenced and compared with the reference phytoplasma sequences collected from the database (NCBI). 16S rDNA sequences of Dharwad chickpea phytoplasma shared the highest nucleotide identity of (>98%) with Periwinkle phyllody16SrII-E (EU096500). This study indicated the association of ‘Candidatus Phytoplasma aurantifolia’ the 16SrII-E group infecting chickpea from Northern Karnataka.

Characterization of Arcanobacterium abortisuis by phenotypic properties and by sequencing the 16S–23S rDNA intergenic spacer region

2011 ◽  
Vol 148 (2-4) ◽  
pp. 431-433 ◽  
Author(s):  
H. Ülbegi-Mohyla ◽  
A.A. Hassan ◽  
M. Hijazin ◽  
J. Alber ◽  
C. Lämmler ◽  
...  
Keyword(s):  
Intergenic Spacer ◽  
Phenotypic Properties ◽  
Intergenic Spacer Region ◽  
Rdna Intergenic Spacer
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