Effect of a calcitonin gene-related peptide antagonist (CGRP8-37) on skin vasodilatation and oedema induced by stimulation of the rat saphenous nerve

From this study, we predicted that the human calcitonin gene-related peptide (hCGRP) fragment hCGRP-(8-37) would be a selective antagonist for CGRP receptors but an agonist for calcitonin (CT) receptors. In rat liver plasma membrane, where CGRP receptors predominate and CT appears to act through these receptors, hCGRP-(8-37) dose dependently displaced 125I-[Tyr0]rat CGRP binding. However, hCGRP-(8-37) had no effect on adenylate cyclase activity in liver plasma membrane. Furthermore, hCGRP-(8-37) inhibited adenylate cyclase activation induced not only by hCGRP but also by hCT. On the other hand, in LLC-PK1 cells, where calcitonin receptors are abundant and CGRP appears to act via these receptors, the bindings of 125I-[Tyr0]rat CGRP and 125I-hCT were both inhibited by hCGRP-(8-37). In contrast to liver membranes, interaction of hCGRP-(8-37) with these receptors led to stimulation of adenosine 3',5'-cyclic monophosphate (cAMP) production in LLC-PK1 cells, and moreover, this fragment did not inhibit the increased production of cAMP induced not only by hCT but also by hCGRP. Thus hCGRP-(8-37) appears to be a useful tool for determining whether the action of CGRP as well as that of CT is mediated via specific CGRP receptors or CT receptors.

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The calcitonin gene-related peptide antagonist CGRP8–37 increases the latency to withdrawal responses in rats

Brain Research ◽

10.1016/0006-8993(94)90393-x ◽

1994 ◽

Vol 653 (1-2) ◽

pp. 223-230 ◽

Cited By ~ 58

Author(s):

Long-Chuan Yu ◽

Per Hansson ◽

Thomas Lundeberg

Keyword(s):

Calcitonin Gene Related Peptide ◽

Related Peptide ◽

Calcitonin Gene ◽

Peptide Antagonist

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Stimulation of acetylcholine release in myenteric plexus by calcitonin gene-related peptide

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.1990.259.6.g934 ◽

1990 ◽

Vol 259 (6) ◽

pp. G934-G939 ◽

Cited By ~ 1

Author(s):

M. W. Mulholland ◽

S. Jaffer

Keyword(s):

Myenteric Plexus ◽

Acetylcholine Release ◽

Calcitonin Gene Related Peptide ◽

Ach Release ◽

Related Peptide ◽

Calcitonin Gene ◽

Dose Dependent Increase ◽

Dose Dependent ◽

Dependent Mechanism ◽

Stimulation Of

The effects of calcitonin gene-related peptide (CGRP) on acetylcholine (ACh) release from myenteric plexus neurons in primary culture were investigated. CGRP (10(-12) to 10(-6) M) produced a dose-dependent increase in [3H]ACh release. The ACh release caused by CGRP was significantly inhibited (74 +/- 24%) by preincubation with dideoxyadenosine but was increased more than threefold by preincubation with theophylline. Incubation of myenteric plexus neurons with CGRP (10(-8) M) in the presence of diltiazem (10(-5) M) or in a calcium-free medium markedly reduced [3H]ACh release. CGRP potentiated [3H]ACh release stimulated by potassium or substance P but not by cholecystokinin octapeptide or forskolin. The results demonstrate that CGRP cause release of ACh from guinea pig myenteric plexus neurons and suggest that the peptide acts through an adenosine 3',5'-cyclic monophosphate-dependent mechanism that involves neuronal calcium channels.

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Effect of calcitonin gene-related peptide antagonist on the cardiovascular events, mortality, and prostaglandin E2 production by nitrate-induced tolerant rats with acute myocardial infarction

The EPMA Journal ◽

10.1186/s13167-016-0055-5 ◽

2016 ◽

Vol 7 (1) ◽

Cited By ~ 4

Author(s):

Tamar Kezeli ◽

Tamari Rukhadze ◽

Nikoloz Gongadze ◽

Galina Sukoyan ◽

Nino Dolidze ◽

...

Keyword(s):

Myocardial Infarction ◽

Acute Myocardial Infarction ◽

Prostaglandin E2 ◽

Cardiovascular Events ◽

Calcitonin Gene Related Peptide ◽

Related Peptide ◽

Calcitonin Gene ◽

Peptide Antagonist

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Cortical Spreading Depression Does Not Result in the Release of Calcitonin Gene-Related Peptide into the External Jugular Vein of the Cat: Relevance to Human Migraine

Cephalalgia ◽

10.1046/j.1468-2982.1993.1303180.x ◽

1993 ◽

Vol 13 (3) ◽

pp. 180-183 ◽

Cited By ~ 29

Author(s):

Richard D Piper ◽

Lars Edvinsson ◽

Rolf Ekman ◽

Geoffrey A Lambert

Keyword(s):

Migraine With Aura ◽

Cortical Spreading Depression ◽

Spreading Depression ◽

Jugular Vein ◽

Calcitonin Gene Related Peptide ◽

External Jugular Vein ◽

Related Peptide ◽

Calcitonin Gene ◽

Vasoactive Peptide ◽

Stimulation Of

There is circumstantial evidence that cortical spreading depression (SD) may account for the scotoma and the “spreading cortical oligemia” seen during migraine with aura. It has been shown that calcitonin gene-related peptide (CGRP) is increased in blood taken from the external jugular vein (EJV) in humans during migraine and after stimulation of the trigeminal ganglion. To test the hypothesis that cortical SD may elevate the concentration of this vasoactive peptide in the EJV during migraine, we have measured its concentration in the external jugular vein of cats during cortical SD. This study demonstrates that SD has no effect on the concentration of CGRP either during the passage of a wave of spreading depression across the cortex or, 60 min later, during the period of post-SD cortical oligemia.

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