Researches on the Detergent Action of Soap

An investigation of the biochemical effects of L-lysophosphatidylcholine (1-acyl-sn-glycerol-3-phosphorylcholine) requires as a control for its detergent action the metabolically inert, unnatural enantiomer, D-lysophosphatidylcholine (3-acyl-sn-glycerol-1-phosphorylcholine). The latter can be easily prepared in milligram quantities from the commercially available DL-phosphatidylcholine. For this purpose rac-1,2-dipalmitoyl-sn-glycerol-3-phosphorylcholine is solubilized with the aid of one-half its weight of taurocholate and hydrolyzed with phospholipase A2 to completion and the intact residual 2,3-dipalmitoyl-sn-glycerol-1-phosphorylcholine recovered by chromatography. The pure D-phosphatidylcholine is then subjected to methanolysis in the presence of octylamine and the product isomerized with glacial acetic acid. The 3-palmitoyl-sn-glycerol-1-phosphorylcholine thus obtained is chromatographically pure, possesses correct optical rotation and NMR spectrum, and is resistant to hydrolysis by phospholipase A2 following reacylation to the diacylphosphatide. Acylation of the 1-sn-lysophosphatide provides a convenient method for preparation of mixed acid 1-sn-phosphatidylcholines which are not otherwise available.

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The Influence of Soap Hydrolysis on Detergent Action

Journal of the Society of Dyers and Colourists ◽

10.1111/j.1478-4408.1947.tb02457.x ◽

2008 ◽

Vol 63 (4) ◽

pp. 107-109 ◽

Cited By ~ 1

Author(s):

K. Tomlinson

Keyword(s):

Detergent Action

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Myometrial estrogen and progesterone receptor binding in pregnancy: Inhibition by the detergent action of phospholipids

The Journal of Steroid Biochemistry and Molecular Biology ◽

10.1016/0960-0760(94)00175-l ◽

1995 ◽

Vol 52 (3) ◽

pp. 287-294 ◽

Cited By ~ 11

Author(s):

Martti Pulkkinen ◽

Mauri M. Hämäläinen

Keyword(s):

Progesterone Receptor ◽

Receptor Binding ◽

Detergent Action ◽

Estrogen And Progesterone Receptor ◽

In Pregnancy

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A QUANTITATIVE METHOD FOR MEASURING THE DETERGENT ACTION OF LAUNDRY SUPPLIES

Canadian Journal of Research ◽

10.1139/cjr32-021 ◽

1932 ◽

Vol 6 (3) ◽

pp. 292-305 ◽

Cited By ~ 5

Author(s):

O. M. Morgan

Keyword(s):

Quantitative Method ◽

Soap Concentration ◽

Detergent Action ◽

Standard Soil

An improved type of standard soil for measuring and comparing the detergent efficiencies of soaps has been developed. The technique of application of this soil to white, desized cotton sheeting has been studied with respect to obtaining a uniform product which is sensitive to small differences in washing procedure, both chemical and mechanical. The effects of time, temperature, soap concentration, and various rates of agitation have been investigated in connection with this soil. The results prove its sensitivity.A miniature wash wheel has been constructed for use in the above work. It exhibits several improvements over small machines of this type formerly used.

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