The effect of storing whole blood at 22°C for up to 24 hours with and without rapid cooling on the quality of red cell concentrates and fresh-frozen plasma

Latar belakang.Aplikasi transfusi darah dalam klinis sehari-hari dapat sebagai terapi pengganti maupun suportif. Sesuai pertimbangan risiko dan manfaat tranfusi komponen darah seperti thrombocyt concentrate(TC) packed red cell(PRC), fresh frozen plasma(FFP), platelete rich plasma (PRP), dan cryoprecipitate/kriopresipitat lebih direkomendasikan daripada whole blood(WB). Tujuan.Mengetahui hubungan antara latar belakang penyakit dengan penggunaan transfusi komponen darah.Metode.Penelitian retrospekstif dilakukan di Ruang Anak Rumah Sakit Dr Kariadi Semarang. Data diperoleh dari register bank darah rumah sakit tahun 2008-2010. Latar belakang penyakit ditentukan dengan mengidentifikasi diagnosis pada setiap kasus transfusi. Komponen darah yang diteliti PRC, TC, FFP, PRP, dan kriopresipitat. Utilisasi dengan menghitung total jumlah komponen darah yang dipakai per diagnosis penyakit, dan rerata pemakaian produk darah per jumlah kasus terindikasi transfusi per tahun. Analisis uji statistik hubungan dengan menggunakan Chi square.Hasil.Terdapat peningkatan rerata utilisasi darah 5678 unit darah per tahun. Terdapat peningkatan penggunaan selama 3 tahun terakhir, secara berurutan adalah 3751, 6496, dan 6787 unit darah (p<0.001). Komponen darah yang paling banyak digunakan berturut-turut adalah TC 3228 unit, PRC 1682 unit, FFP 295 unit, PRP 224 unit, dan cryo133 unit. Pasien leukemia merupakan pengguna komponen darah terbanyak dengan rerata pemakaian per tahun 2098 unit, diikuti oleh sepsis 893 unit, dan thalassemia 568 unit. Rasio kebutuhan PRC terbanyak untuk kasus penyakit jantung (2,23) diikuti penyakit ginjal (2,25) dan thalassemia (1,7). Untuk penggunaan TC, terbanyak berturut-turut adalah ITP (14,70 unit), anemia aplastik (9,8 unit), dan leukemia (6 unit). Terdapat hubungan antara diagnosis penyakit dengan penggunaan transfusi komponen PRC, TC, dan plasma (p<0,001).Kesimpulan.Terdapat hubungan antara latar belakang penyakit penyebab dengan penggunaan transfusi komponen darah. Leukemia, sepsis, dan thalassemia adalah latar belakang penyakit yang paling banyak menggunakan transfusi komponen darah. Berturut turut komponen darah yang banyak digunakan adalah konsentrat trombosit, komponen sel darah merah, serta plasma darah segar.

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QUALITY OF REPEATEDLY FREEZE-THAWED FRESH FROZEN PLASMA DERIVED FROM 400 ml OF WHOLE BLOOD

Japanese Journal of Transfusion and Cell Therapy ◽

10.3925/jjtc.61.403 ◽

2015 ◽

Vol 61 (3) ◽

pp. 403-408 ◽

Cited By ~ 2

Author(s):

Akihiro Fuchizaki ◽

Mami Matsumoto ◽

Masayuki Shiba ◽

Masahiro Satake ◽

Kenji Tadokoro

Keyword(s):

Whole Blood ◽

Fresh Frozen Plasma ◽

Fresh Frozen ◽

Frozen Plasma

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A Comparison Study of the Blood Component Quality of Whole Blood Held Overnight at 4°C or Room Temperature

Journal of Blood Transfusion ◽

10.1155/2013/523539 ◽

2013 ◽

Vol 2013 ◽

pp. 1-7 ◽

Cited By ~ 1

Author(s):

Shichun Wang ◽

Tiantian Wang ◽

Yahan Fan ◽

Shan Huang ◽

Zhongmei Yi ◽

...

Keyword(s):

Whole Blood ◽

Biochemical Parameters ◽

Room Temperature ◽

Coagulation Factors ◽

Fresh Frozen Plasma ◽

Blood Component ◽

Comparison Study ◽

Fresh Frozen ◽

Frozen Plasma

Background. The use of plasma frozen within 24 hrs is likely to increase. Whole blood (WB) and buffy coats (BCs) can be held for a few hrs or overnight before processing. Methods. Twenty-four bags of WB for plasma and 12 bags for platelet (PLT) concentrates were collected. The fresh frozen plasma (FFP) was prepared within 6 hrs. I-FP24 and II-FP24 samples were prepared either from leukodepleted WB that was held overnight or from WB that was held overnight before leukodepletion. The PLT concentrates (PCs) were prepared from BCs within 6 hrs (PC1) and within 18 to 24 hrs (PC2). The typical coagulation factors and some biochemical parameters were determined. Results. Compared to the FFP samples, the levels of FVII and FVIII in the I-FP24 and II-FP24 samples decreased significantly. The pH, Na+, LDH, and FHb levels differed significantly between II-FP24 and FFP. Compared to PC1, PC2 exhibited lower pH, pO2, and Na+ levels, a higher PLT count, and increased pCO2, K+, Lac, and CD62P expression levels. Conclusion. FP24 is best prepared from WB that was stored overnight at 4°C and then leukodepleted and separated within 24 hrs. PCs are best produced from BCs derived from WB that was held overnight at room temperature.

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Effect of Whole Blood Storage on Factor VIII Recovery in Fresh Frozen Plasma and Cryoprecipitate

Vox Sanguinis ◽

10.1159/000462046 ◽

1996 ◽

Vol 71 (3) ◽

pp. 150-154 ◽

Cited By ~ 5

Author(s):

D. P. Allersma ◽

R. M. R. Imambaks ◽

L. J. Meerhof

Keyword(s):

Factor Viii ◽

Whole Blood ◽

Fresh Frozen Plasma ◽

Blood Storage ◽

Fresh Frozen ◽

Frozen Plasma

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Quality Assurance in Hemapheresis: Quality of Fresh Frozen Plasma

The International Journal of Artificial Organs ◽

10.1177/039139889301605s51 ◽

1993 ◽

Vol 16 (5_suppl) ◽

pp. 226-228

Author(s):

G.M. Baldini ◽

M.G. Silvestri

Keyword(s):

Quality Assurance ◽

Fresh Frozen Plasma ◽

Fresh Frozen ◽

Frozen Plasma

The Authors analyse how established criteria for quality assurance of FFP may be applied to apheresis plasma produced for fractionation into derivatives.

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Hemostatic Effects of Fresh Frozen Plasma May be Maximal at Red Cell Ratios of 1:2

Journal of Trauma and Acute Care Surgery ◽

10.1097/ta.0b013e318202e486 ◽

2011 ◽

Vol 70 (1) ◽

pp. 90-96 ◽

Cited By ~ 87

Author(s):

Ross Davenport ◽

Nicola Curry ◽

Joanna Manson ◽

Henry DeʼAth ◽

Amy Coates ◽

...

Keyword(s):

Fresh Frozen Plasma ◽

Red Cell ◽

Fresh Frozen ◽

Hemostatic Effects ◽

Frozen Plasma

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Primary Plasma Therapy For Thrombotic Thrombocytopenic Purpura

10.1055/s-0038-1653324 ◽

1981 ◽

Author(s):

D C Case

Keyword(s):

Platelet Count ◽

Blood Cells ◽

Reticulocyte Count ◽

Fresh Frozen Plasma ◽

Red Cell ◽

Peripheral Smear ◽

Plasma Therapy ◽

Packed Red Blood Cells ◽

Fresh Frozen ◽

Frozen Plasma

A 25-year old male was admitted for an episode of right sided headache and subsequent generalized seizure. On admission his temperature was 37.6°. He had generalized petechiae and conjunctival hemorrhages. Organomegaly and lymphadenopathy were absent. There was mild left sided weakness. The Hgb. was 6.9 g/dl., reticulocyte count 10%, WBC 11,500/mm3, and platelet count 10,000/mm3. There were numerous schistocytes on the peripheral smear; bone marrow revealed panhyperplasia. Coagulation studies were normal. The BUN was 30, and the creatinine 1.7 mg/dl. Plasma was positive for Hgb. CT scan was negative for gross intracranial bleeding. The diagnosis of T.T.P. was made. On admission, the patient received 10 units of platelets and 2 units of packed red blood cells. He did not require further red cell or platelet transfusions during the rest of his hospital course. He was then started on infusions of fresh-frozen plasma. He then received one unit every 3 hours for 6 days, one unit every 6 hours for 2 days, then one unit every 12 hours for 2 days and finally 1 unit daily for 5 days. The response was immediate. After the infusions were started, the hematologic parameters steadily improved. The patient’s hematuria rapidly improved. Further CNS symptoms did not appear. The patient’s Hgb. was 12 g/dl, and reticulocyte count was 2.5% by the 9th day. His platelet count was normal by the 4th day. The patient was discharged on the 15th day. Infusions of plasma were discontinued at the time of discharge. The patient required plasma therapy 4 weeks later for recurrent thrombocytopenia (50,000/mm3). The patient has remained normal for 9 months since therapy and further plasma has not been required. Primary plasma therapy for T.T.P. as sole treatment should be further studied.

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