Electron-Microscopic Studies onTheileria ovisRodhain, 1916: Development of Kinetes in the Gut of the Vector Tick,Rhipicephalus evertsi evertsiNeumann, 1897, and Their Transformation Within Cells of the Salivary Glands

1979 ◽  
Vol 26 (3) ◽  
pp. 377-385 ◽  
Author(s):  
HEINZ MEHLHORN ◽  
EBERHARD SCHEIN ◽  
MANFRED WARNECKE
Keyword(s):  
Salivary Glands ◽  
Electron Microscopic ◽  
Vector Tick ◽  
Microscopic Studies

Immunogold labeling of bluetongue virus in cryosections from Culicoides variipennis (Coquillett) salivary gland

1988 ◽  
Vol 46 ◽  
pp. 372-373
Author(s):  
R. A. Nunamaker ◽  
B. C. Wick ◽  
C. E. Nunamaker
Keyword(s):  
Salivary Glands ◽  
Electron Microscopic ◽  
Important Species ◽  
Biting Midge ◽  
Viral Particles ◽  
Natural Hosts ◽  
Microscopic Studies ◽  
Primary Vector ◽  
Culicoides Variipennis ◽  
Replicative Cycle

When an arbovirus enters its arthropod host during a viremic blood meal it quickly reaches the midgut where virus penetration of the host parenchyma and infection of epithelial cells apparently occur. Subsequently, viral particles enter the arthropod's hemolymph and are transported to other tissues which may then become infected. Previous studies have demonstrated that when the arbovirus reaches the salivary glands it increases in titer and persists over long periods, often throughout the life of the vector.It is this persistent presence and associated continual shedding of virus into saliva which is one reason that certain arthropods are extremely efficient biological vectors.Culicoides variipennis (Coquillett) is probably the most economically important species of biting midge in the U.S. due to its involvement in the transmission of bluetongue (BT) disease of sheep, cattle and ruminant wildlife. Although there have been numerous electron microscopic studies of many mosquito-borne viruses in their natural hosts, virtually nothing is known about the replicative cycle of BTV in the salivary glands of its primary vector.

scholarly journals TO THE ULTRASTRUCTURAL ORGANIZATION OF EPITHELIOCYTES OF EXOCRINE GLANDS

2021 ◽  
Vol 245 (1) ◽  
pp. 108-112
Author(s):  
N.V. Momot ◽  
◽  
Y.A. Kolina ◽  
I.L. Kamliya ◽  
◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Salivary Glands ◽  
Standard Technique ◽  
Ultrastructural Organization ◽  
Exocrine Glands ◽  
Electron Microscopic ◽  
Large White ◽  
Microscopic Studies ◽  
Sexually Mature ◽  
Large White Pig

Pieces of the sublingual multi-duct salivary glands of a domestic sexually mature large white pig were fixed in a 4 % paraform solution in 0.1 M phosphate buffer (pH = 7.4) with addi-tional fixation in a 1 % OsO4 solution, dehydrated in alcohols of increasing concentration. Taking into account the recommendations of G. Gayer, pieces of organs were poured into araldite accord-ing to the standard technique. Contrasting was performed according to Reynolds. In the cytoplasm of the mucocytes of the sublingual multi-duct salivary gland, the agranu-lar endoplasmic reticulum predominates, which gives oxyphilic staining. According to electron microscopic studies of mucous glandulocytes in the acini of the sub-lingual salivary glands of domestic pigs, the secretory vacuoles of the cytoplasm are large, with pro-nounced electron-dense membranes. The content of vacuoles in mucocytes of one acinus is differ-ent.

The effects of serotonin and dopamine on salivary secretion by isolated cockroach salivary glands

1996 ◽  
Vol 199 (2) ◽  
pp. 407-413 ◽  
Author(s):  
F Just ◽  
B Walz
Keyword(s):  
Salivary Glands ◽  
Periplaneta Americana ◽  
Morphological Changes ◽  
Salivary Secretion ◽  
Dependent Manner ◽  
Electron Microscopic ◽  
Microscopic Studies ◽  
Protein Rich ◽  
Dose Dependent ◽  
Stimulation Of

We have studied the effects of 3-hydroxytyramine (dopamine) and 5-hydroxytryptamine (serotonin) on (1) the rates of salivation from isolated salivary glands of the cockroach Periplaneta americana, (2) the protein content of the saliva, and (3) the ultrastructure of the salivary gland epithelium. The rates of neurotransmitter-induced salivation varied in a dose-dependent manner within the concentration range 10(-9) to 10(-4) mol l-1. Half-maximal secretory rates were induced by 6x10(-7) mol l-1 serotonin and 1.1x10(-7) mol l-1 dopamine. Stimulation of the glands by serotonin resulted in the production of a protein-rich saliva, whereas saliva was protein-free after stimulation by dopamine. Electron microscopic studies revealed that the central cells, which are believed to produce the proteinaceous components of the saliva, secrete their vesicular content after stimulation by 10(-6) mol l-1 serotonin for 20 min. In contrast, no morphological changes could be detected after stimulation by 10(-6) mol l-1 dopamine. These data indicate that dopamine stimulates only the secretion of the fluid component of the saliva, whereas serotonin is necessary to stimulate secretion of the proteinaceous components.

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