Effect of Coriander Plants on Human Emotions, Brain Electrophysiology, and Salivary Secretion

Coriander is a popular herb with versatile applications. However, the current research about coriander medicinal values have been mainly focusing on its extracts while lacking in the relationship between living coriander plants and emotion. Therefore, this study aims to investigate the effects of coriander plants on human emotions and physiological activities. The results showed that the main Volatile organic compounds (VOCs) of coriander plants were 2-ethyl-1-hexanol, d-limonene, eucalyptol, benzyl alcohol, Isophorone, dimethyl glutarate, α-terpineol, styrene, methyl methacrylate, α-pinene. Coriander plants could significantly reduce the angry sub-scores, alpha amylase and amino acids (arginine, proline, histidine, and taurine) concentrations in saliva. Theta (4–8 Hz) band activity of the cerebral cortex was significantly enhanced. Moreover, taurine significantly positively correlated with anger and negatively correlated with vigor. All the results signified that coriander plant could influence the activity of brain electrophysiological and salivary secretion through its VOCs to improve people’s negative emotions.

Comparative Phosphoproteomic Profiling in Prefrontal Cortex of Prenatal Stressed Offspring Rats

Background: Many investigations indicate that prenatal stress caused depressive-like disturbances in offspring rats. The underlying pathogenic mechanisms have not yet been fully unravelled. The PFC has been shown to play a role in susceptibility to stress during fetal, thus we focus our attention on differential protein phosphorylation in this region of PS-S(Susceptibility to PS) offspring rats.Method: SPT was used to screen for susceptibility to PS. The validity of prenatally stressed model was verified by other common depression-like behaviors. We used MS-based TMT quantitative proteomics in combination with phosphopeptide enrichment method to compare phosphoproteomic profiling in prefrontal cortex of of PS-S and CON offspring rats. Results: Totally, 3418 phosphoproteins, 8404 phosphopeptides and 12175 phosphosites were identified in this analysis. According to the screening criteria, 902 phosphopeptides increased and 609 decreased in the PFC of PS-S group compared to the control rats. GO enrichment analysis indicated that the main enriched terms in CC category were ‘synapse part’, ‘myelin sheath’, ‘synapse’, ‘neuron part’ and ‘axon’. The phosphorproteins enriched in MF and BP category were mainly related to cytoskeleton and projection morphogenesis associated proteins. KEGG pathway enrichment analyses identified 30 significant KEGG pathways, the top five pathways included salivary secretion, Endocrine and other factor-regulated calcium reabsorption, Pancreatic secretion and Insulin secretion. Motifs such as......_S_P...RR, ......_S_PE...., ......_S_PV...., ......_S_P.H...and ..S..._S_PT....were the top five motifs enriched in phosphorylated sites.Conclusion: PS may induce depressive-like behaviors in offspring rats through regulating the phosphorylation of protein mainly related to synapse, myelin sheath, neuron and cytoskeleton. And the phosphorylation of related proteins may act as key pathogenic hits. Data are available via ProteomeXchange with identifier PXD026563.

Antioxidant-Rich Natural Raw Materials in the Prevention and Treatment of Selected Oral Cavity and Periodontal Diseases

Antioxidant-rich natural raw materials have been used for thousands of years in traditional medicine. In the past decade, there has been increasing interest in naturotherapy, which is a practice of using products with a natural origin. Natural products can be effective in the treatment and prevention of oral and dental diseases, among others. Such raw materials used in dentistry are characterized by antioxidant, anti-inflammatory, antibacterial, antiviral, antiedematous, astringent, anticoagulant, dehydrating, vitaminizing, and—above all—regenerative properties. Reports have shown that a relationship exists between oral diseases and the qualitative and quantitative composition of the microbiota colonizing the oral cavity. This review aimed to analyze the studies focusing on the microbiome colonizing the oral cavity in the context of using natural raw materials especially herbs, plant extracts, and isolated biologically active compounds as agents in the prevention and treatment of oral and periodontal diseases such as dental caries as well as mucosal changes associated with salivary secretion disorder. The present work discusses selected plant ingredients exhibiting an antioxidant activity with potential for the treatment of selected oral cavity and periodontal diseases.

Transcutaneous Electrical Nerve Stimulation: A Review of Dental Applications

Transcutaneous electrical nerve stimulation or TENS is a nonpharmacologic method that is used to alleviate pain from various acute and chronic conditions. TENS works through activation of descending inhibitory neurons of the central nervous system. In dentistry, TENS has applications for pain reduction in temporomandibular disorders and postherpetic neuralgia. It has also been studied for its role in increasing salivary secretion in subjects with xerostomia and as a local anesthetic agent in pediatric dentistry. Its wide applications in the orofacial region make it an important treatment modality. The purpose of the present review was to describe the general principles and its applications in dentistry along with its indications, advantages, and disadvantages. A web-based search was conducted using the keywords “transcutaneous electrical nerve stimulation,” “temporomandibular joint,” “TENS,” “TMJ,” “orofacial pain,” and “Management.” The manuscripts thus obtained were evaluated and used for compiling the present narrative review. The present review focuses on the mechanism of action, applications, and effectiveness of TENS in the management of diseases of the maxillofacial region.

Stimulated saliva composition in patients with cancer of the head and neck region

Background To analyse over time changes in stimulated whole saliva regarding total protein, Immunoglobulin A (IgA), and mucin type O-glycans (mostly MUC5B and MUC7) in head and neck cancer patients. Methods 29 dentate patients (20 men and 9 women, 59 ± 8 years) treated with curative radiation therapy and chemotherapy for cancer of the head and neck region were included. The stimulated whole salivary secretion rate was determined and saliva collected at four time-points: at pretreatment, and at 6 months, 1 and 2 years post treatment. The total protein concentration was determined spectrophotometrically by using Bicinchoninic Acid assay and Immunoglobulin A (IgA) by using ELISA technique. Glycosylation pattern of salivary mucins was determined in samples collected pre- and post treatment by using LC/MS electrospray and mucin content quantified using SDS-AgPAGE gels and PAS staining. Results Compared with pretreatment, the total protein concentration was increased already at 6 months post treatment (p < 0.01), and continued to increase up to 2 years post treatment (p < 0.001). During that period no significant changes in IgA concentration was detected. At pretreatment, the output/min of both total protein and IgA was significantly higher than at all time-points post treatment. Saliva from the cancer patients showed a low abundance/no detectable MUC7, while the MUC5B level remained, compared to saliva from a healthy control. The glycomic analysis showed that the percentage of core 2 O-glycans was increased as core 1, 3 and 4 O-glycans were decreased. The level of sialylation was higher at 6 months post treatment, while sulfation was lower. Conclusion A decreased output per minute of proteins at decreased salivary secretion rate, as well as reduced sulfation of MUC5B at 6 months post treatment tended to correlate with the patients’ experience of sticky saliva and oral dryness. At 2 years post treatment, the decreased amount of IgA combined with a lowered salivary secretion rate indicate a reduced oral defense with increased risk of oral infections.

Application of Peppermint Water Spray Combined With Cucumber Slice External Application or Lip Balm and Conventional Dipping in ICU Dry Mouth Patients

to analyze the effect of peppermint water spray combined with external application of cucumber slice or lip balm and conventional dipping in patients with dry mouth in intensive care unit (ICU). Methods: 120 patients with secondary xerostomia in our hospital from January 2018 to December 2019 were selected as the subjects. According to the different treatment methods, the patients were divided into A group (Peppermint water spray combined with cucumber slices for external application), group B (Peppermint water spray combined with lip gloss smear), group C (cotton swab dipped in normal saline), 40 cases in each group, and the saliva secretion and dry mouth before and after intervention in three groups were compared between the three groups. The degree of self-evaluation score and the evaluation of throat comfort and satisfaction after intervention. Results: after the intervention, the self-evaluation scores of salivary secretion and dry mouth degree of patients in group A and B were significantly higher than those before the intervention (P < 0.01), the self-evaluation scores of salivary secretion and dry mouth degree of patients in group A were significantly higher than those in group B and C (P < 0.01), and the self-evaluation scores of salivary secretion and dry mouth degree of patients in group B were significantly higher than those in group C (P < 0.01). After intervention, the total effective rate of improvement of throat comfort in group A was 87.50%, which was significantly higher than 40.00% in group B and 0.00% in group C (P < 0.01). The total effective rate of improvement of throat comfort in group B was significantly higher than that in group C (P < 0.01). 97.50% of the patients in group A were significantly higher than 72.50% in group B and 2.50% in group C (P < 0.01), and the patients in group B were significantly higher than those in group C (P < 0.01). Conclusion: homemade peppermint water spray combined with external application of cucumber spray can effectively improve the symptoms of dry mouth and throat discomfort in patients with ICU, improve their satisfaction with treatment, and is conducive to the recovery of patients’ diseases, which is worthy of clinical promotion and application.

Xerostomia among elderly patients – systematic review

Introduction and purpose: Xerostomia is a salivary secretion malfunction that affects from 1 to 29% of the population. It is a common problem among elderly patients, however undeniably it still remains an underdiagnosed issue. Searching among available literature in the PubMed database with the following phrases: xerostomia; elderly, the aim of this article was to provide a broad review on the underdiagnosed problem which is xerostomia among elderly patients. Description: A group at risk of developing xerostomia are people over 65 years old and women in the perimenopausal period. Although dry mouth varies in etiology, geriatric patients mostly develop xerostomia as a result of head and neck radiotherapy, Sjögren syndrome or medication treatment. Untreated symptoms can lead to severe issues which heavily impact not only oral health of the patient, but also their everyday life quality, since xerostomia may lead into difficulty of speaking, swallowing and tasting. The plan of treatment is influenced by the etiology of the case, however it is aimed to stimulate salivary flow and eradicate the use of unnecessary medication which may cause dry mouth. Prevention of dry mouth is based on maintenance of good oral hygiene. Conclusions: Diagnosing the problem early can prevent patients from suffering the consequences of chronic xerostomia, therefore awareness should be brought to this issue. Dentist could also highly improve the quality of xerostomic patient’s life, if the chosen treatment significantly improved patient’s symptoms.

The characteristics of proteome and metabolome associated with contrasting sperm motility in goat seminal plasma

Sperm motility is an index tightly associated with male fertility. A close relationship between seminal plasma and sperm motility has been confirmed. This study was to assess the protein and metabolite profiles of seminal plasma obtained from adult goats with high or low sperm motility using the proteomic and metabolomic strategies. In total, 2098 proteins were found. 449 differentially abundant proteins (DAPs) were identified, and 175 DAPs were enriched in the high motility group. The obtained DAPs primarily exist in cytoplasma and extra-cellular portion. The Gene Ontology enrichment analysis demonstrated the main functional roles of these DAPs in regulating biological process, metabolic process of organic substances, cellular-metabolic process, primary-metabolic process, metabolic process of nitrogen compounds, etc. Additionally, the Kyoto-Encyclopedia of Genes and Genomes (KEGG) analysis revealed that these DAPs were primarily involved in phosphatidylinositol signaling system, salivary secretion, proteasome, apoptosis, mitophagy-animal, etc. Aided by the parallel reaction monitoring technology, the abundance changing pattern of 19 selected DAPs was consistent with that of the corresponding proteins obtained by TMT. A total of 4603 metabolites were identified in seminal plasma. 1857 differential metabolites were found between the high motility group and the low motility group, and 999 metabolites were up-regulated in the high motility group. The KEGG analysis demonstrated the primary involvement of the differential metabolites in metabolic and synthetic activities. In conclusion, we first established the proteome and metabolome databank of goat seminal plasma, detecting some proteins and metabolites which may affect sperm motility. This study will be valuable for understanding mechanisms leading to poor sperm motility.

Highly Localized intracellular Ca2+ signals promote optimal salivary gland fluid secretion

Salivary fluid secretion involves an intricate choreography of membrane transporters to result in the trans-epithelial movement of NaCl and water into the acinus lumen. Current models are largely based on experimental observations in enzymatically isolated cells where the Ca2+ signal invariably propagates globally and thus appears ideally suited to activate spatially separated Cl and K channels, present on the apical and basolateral plasma membrane, respectively. We monitored Ca2+ signals and salivary secretion in live mice expressing GCamp6F, following stimulation of the nerves innervating the submandibular gland. Consistent with in vitro studies, Ca2+ signals were initiated in the apical endoplasmic reticulum. In marked contrast to in vitro data, highly localized trains of Ca2+ transients that failed to fully propagate from the apical region were observed. Following stimuli optimum for secretion, large apical-basal gradients were elicited. A new mathematical model, incorporating these data was constructed to probe how salivary secretion can be optimally stimulated by apical Ca2+ signals.