scholarly journals Helicobacter heilmannii can induce gastric lymphoid follicles in mice via a Peyer's patch-independent pathway

2010 ◽  
Vol 60 (2) ◽  
pp. 156-164 ◽  
Author(s):  
Kentaro Nobutani ◽  
Masaru Yoshida ◽  
Shin Nishiumi ◽  
Yosuke Nishitani ◽  
Tetsuya Takagawa ◽  
...  
Keyword(s):  
Peyer’S Patch ◽  
Peyer's Patch ◽  
Lymphoid Follicles ◽  
Helicobacter Heilmannii

scholarly journals Lymphocyte Depletion in Ileal Peyer’s Patch Follicles in Lambs Infected with Eimeria ovinoidalis

2002 ◽  
Vol 9 (1) ◽  
pp. 83-91 ◽  
Author(s):  
Mona Aleksandersen ◽  
Kai-Inge Lie ◽  
Bjørn Gjerde ◽  
Thor Landsverk
Keyword(s):  
Carbonic Anhydrase ◽  
Peyer’S Patch ◽  
Peyer's Patch ◽  
Ki 67 ◽  
Lymphoid Follicles ◽  
Lymphocyte Depletion ◽  
Follicle Associated Epithelium ◽  
Follicle Size ◽  
Reticular Cells ◽  
Labeling Method

ABSTRACT A total of 14 lambs were experimentally infected with Eimeria ovinoidalis in two separate experiments in two consecutive years. Nine lambs served as uninoculated controls. Material was collected from the ileum 2 weeks after infection in eight lambs and 3 weeks after infection in six lambs. Lambs examined 2 weeks after infection had normal follicles. After three weeks, the follicle-associated epithelium covering the lymphoid follicles of the ileal Peyer’s patches showed fusions with adjacent absorptive epithelium, focal hyperplasia, and occasionally necrosis. Macrogametes, microgamonts, and oocysts were often found in the follicle-associated epithelium and the dome region. Various degrees of lymphocyte depletion were present in the ileal lymphoid follicles in all six infected lambs 3 weeks after infection, and four lambs had decreased follicle size. Reduced staining for leukocyte common antigen (CD45), B-cell markers, and the proliferation marker Ki-67 was present in these lambs. Application of the terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling method for apoptotic cells revealed decreased staining in the ileal lymphoid follicles 3 weeks after infection. A marker of follicular dendritic cells, 5′- nucleotidase, showed increased reactivity, probably due to condensation of reticular cells following loss of follicle lymphocytes. Reduced staining for carbonic anhydrase in the follicle-associated epithelium and the domes was present in all six lambs examined 3 weeks after infection, indicating decreased production of carbonic anhydrase-reactive 50-nm particles and a decreased lymphoproliferative stimulus. In conclusion, the present study shows that severe E. ovinoidalis infection in lambs causes lesions of the follicle-associated epithelium and may result in lymphocyte depletion and atrophy of the ileal Peyer’s patch follicles.

Three-Dimensional Reconstruction of Murine Peyer's Patches from Immunostained Cryosections

2013 ◽  
Vol 20 (1) ◽  
pp. 198-205 ◽  
Author(s):  
Sarita Ahlawat ◽  
Magdia De Jesus ◽  
Kedar Khare ◽  
Richard A. Cole ◽  
Nicholas J. Mantis
Keyword(s):  
Three Dimensional ◽  
Cell Types ◽  
Peyer's Patches ◽  
Source Image ◽  
Peyer’S Patches ◽  
Peyer’S Patch ◽  
Peyer's Patch ◽  
Lymphoid Follicles ◽  
Dimensional Reconstruction ◽  
Specialized Tissue

AbstractPeyer's patches, macroscopic aggregates of lymphoid follicles present throughout the small intestines of humans and other mammals, are considered the gateway through which luminal dietary antigens and microbes are sampled by the mucosal immune system. The cellular make-up of Peyer's patch lymphoid follicles is not only complex, but highly dynamic, as there are at least four major cell types that are known to migrate in response to antigenic stimulation. In an effort to capture the complexity and dynamic nature of this specialized tissue, here we report the three-dimensional (3D) reconstruction of immunofluorescent-labeled mouse Peyer's patch cryosections. The technology that enabled the stacking and linear blending of serial cryosections was a novel macro for Fiji, the open source image-processing package based on ImageJ. By simultaneously labeling cryosections for surface markers CD45R, CD3, and CD11c, we provide a 3D image as well as quantitative measures of B-cell, T-cell, and dendritic cell populations at steady state and following exposure to the mucosal adjuvant cholera toxin.

scholarly journals Lymphoid follicles of the ileal Peyer's patch of lambs express low levels of PrP, as demonstrated by quantitative real-time RT-PCR on microdissected tissue compartments, in situ hybridization and immunohistochemistry

2006 ◽  
Vol 87 (11) ◽  
pp. 3463-3471 ◽  
Author(s):  
Lars Austbø ◽  
Arild Espenes ◽  
Ingrid Olsaker ◽  
Charles McL. Press ◽  
Grethe Skretting
Keyword(s):  
Nervous System ◽  
Real Time ◽  
Prion Protein ◽  
Cellular Prion Protein ◽  
Peyer’S Patch ◽  
Peyer's Patch ◽  
Rt Pcr ◽  
Lymphoid Follicles ◽  
Low Levels

The expression level of normal cellular prion protein (PrPC) is thought to influence the transmission of transmissible spongiform encephalopathies (TSEs) from the peripheral entry site to the site of pathological changes in the central nervous system. In many TSEs, the clinical disease is preceded by a period in which the agent accumulates in lymphoid organs, particularly in association with follicular dendritic cells of lymphoid follicles. As the probable route of entry of the TSE agent is via the gut, the expression profile of PrP was examined in well-developed gut-associated lymphoid tissue of lambs, the ileal Peyer's patch, by laser microdissection and real-time RT-PCR. Lymphoid follicles were found to have very low levels of expression, whilst highest levels were detected in the outer submucosa and the muscular layer. These findings were supported by in situ hybridization and immunohistochemistry, which showed specific labelling in nerve cells in ganglia of the submucosal (Meissner's) and myenteric (Auerbach's) plexi of the enteric nervous system. Based on the assumption that potential sites for conversion to the scrapie-related prion protein (PrPSc) should display high levels of expression of PrPC, this study suggests that the accumulation of PrPSc in the lymphoid follicles of the Peyer's patch is not preceded by PrP conversion in the same tissue compartment.

scholarly journals Increased PrP mRNA expression in lymphoid follicles of the ileal Peyer's patch of sheep experimentally exposed to the scrapie agent

2007 ◽  
Vol 88 (7) ◽  
pp. 2083-2090 ◽  
Author(s):  
Lars Austbø ◽  
Arild Espenes ◽  
Ingrid Olsaker ◽  
Charles McL. Press ◽  
Grethe Skretting
Keyword(s):  
Prion Protein ◽  
Cellular Prion Protein ◽  
Expression Level ◽  
Mrna Levels ◽  
Peyer’S Patch ◽  
Peyer's Patch ◽  
High Accumulation ◽  
Lymphoid Follicles ◽  
Tissue Compartments

To understand the functional role of cellular prion protein (PrPC) in the initiation and maintenance of prion disease within the host, it is important to obtain a more detailed understanding of PrPC transcription in tissues during the development of disease. Using an experimental model with oral infection, we examined the effect of scrapie and the accumulation of the scrapie related form of the prion protein (PrPSc) on the expression level of PrP mRNA in the ileal Peyer's patch of sheep. In the early phase of infection, prior to PrPSc accumulation, no effect on the PrP expression was detected. However, it was found that lambs with PrP genotypes associated with high susceptibility for scrapie generally had higher PrP mRNA levels than lambs with less susceptible genotypes. Further, in highly susceptible VRQ/VRQ sheep at a stage of disease with high accumulation of PrPSc, real-time RT-PCR and microdissection were used to investigate levels of PrP mRNA in four different tissue compartments. An increased level of PrP mRNA was found in lymphoid follicles of infected sheep compared with controls, indicating upregulation of PrP expression in the follicles to compensate for the loss of PrPC converted to PrPSc, or that PrPSc accumulation directly or indirectly influences the PrP expression. Still, the PrP expression level in the follicles was low compared with the other compartments investigated, suggesting that although increased PrP expression could contribute to PrPSc accumulation, other factors are also important in the processes leading to accumulation of PrPSc in the follicles.

Lymphoid tissue of the Peyer's patch of the duck ileum

2018 ◽  
Vol 32 (2) ◽  
pp. 348-354
Author(s):  
T. Mazurkevych ◽  
I. Vyshkovska ◽  
N. Hudz
Keyword(s):  
Lymphoid Tissue ◽  
Peyer’S Patch ◽  
Peyer's Patch
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