scholarly journals Use of inhibitors to identify essential cysteine proteinases of Trichomonas vaginalis

2006 ◽  
Vol 149 (1) ◽  
pp. 45-50 ◽  
Author(s):  
Joseph W Irvine ◽  
Michael J North ◽  
Graham H Coombs
Keyword(s):  
Trichomonas Vaginalis ◽  
Cysteine Proteinases

An analysis of the proteinases ofTrichomonas vaginalisby polyacrylamide gel electrophoresis

Parasitology ◽  
1983 ◽  
Vol 86 (1) ◽  
pp. 1-6 ◽  
Author(s):  
G. H. Coombs ◽  
M. J. North
Keyword(s):  
Gel Electrophoresis ◽  
Trichomonas Vaginalis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Cysteine Proteinases ◽  
Polyacrylamide Gels

SUMMARYThe proteinases ofTrichomonas vaginalishave been analysed by electrophoresis in polyacrylamide gels containing denatured haemoglobin. Seven bands of activity were detected indicating multiple proteinases. All of the enzymes were stimulated by 1 mM dithiothreitol and had inhibitor sensitivities characteristic of cysteine proteinases. The enzymes differed significantly, however, with respect to pH optima and relative sensitivities to inhibitors.

Characterization ofTrichomonas vaginalishaemolysis

Parasitology ◽  
1990 ◽  
Vol 101 (2) ◽  
pp. 171-175 ◽  
Author(s):  
D. C. Dailey ◽  
C. Te-Hung ◽  
J. F. Alderete
Keyword(s):  
Incubation Period ◽  
Trichomonas Vaginalis ◽  
Human Erythrocytes ◽  
Haemolytic Activity ◽  
Cysteine Proteinases ◽  
Temperature Dependent ◽  
Chloromethyl Ketone ◽  
Ph Range ◽  
Culture Supernatants ◽  
Dependent Mechanism

The haemolytic activity of liveTrichomonas vaginalisorganisms was investigated. Optimal haemolysis of human erythrocytes was observed at a parasite to erythrocyte ratio of 1:5 during a 2 h incubation period. No haemolytic activity was detected in concentrated culture supernatants after overnight growth of trichomonads or when parasites were separated from erythrocytes by a 3 μm filter, suggesting a contact-dependent mechanism for haemolysis. The haemolytic activity was temperature-dependent and maximal haemolysis occurred at 37 °C. Treatment of trichomonads with metronidazole reduced levels of haemolysis by > 50%. Maximal haemolysis occurred at the pH range of the vagina during trichomoniasis.N-μ-tosyl-L-lysyl-chloromethyl ketone and iodoacetamide, inhibitors of trichomonad cysteine proteinases, reduced the haemolytic activity of live parasites.

The recombinant prepro region of TvCP4 is an inhibitor of cathepsin L-like cysteine proteinases of Trichomonas vaginalis that inhibits trichomonal haemolysis

2015 ◽  
Vol 59 ◽  
pp. 73-83 ◽  
Author(s):  
Rosa Elena Cárdenas-Guerra ◽  
Jaime Ortega-López ◽  
Claudia Ivonne Flores-Pucheta ◽  
Claudia Guadalupe Benítez-Cardoza ◽  
Rossana Arroyo
Keyword(s):  
Trichomonas Vaginalis ◽  
Cathepsin L ◽  
Cysteine Proteinases

Two Trichomonas vaginalis Loci Encoding for Distinct Cysteine Proteinases Show a Genomic Linkage with Putative Inositol Hexakisphosphate Kinase (IP6K2) or an ABC Transporter Gene

2003 ◽  
Vol 50 (s1) ◽  
pp. 702-705 ◽  
Author(s):  
CLAUDIA DEL ROSARIO LEON-SICAIROS ◽  
IZA PEREZ-MARTINEZ ◽  
MARIA ELIZBETH ALVAREZ-SANCHEZ ◽  
IMELDA LOPEZ-VILLASENOR ◽  
ROSSANA ARROYO
Keyword(s):  
Abc Transporter ◽  
Trichomonas Vaginalis ◽  
Cysteine Proteinases ◽  
Transporter Gene ◽  
Inositol Hexakisphosphate ◽  
Genomic Linkage

Trichocystatin-2 (TC-2): An endogenous inhibitor of cysteine proteinases in Trichomonas vaginalis is associated with TvCP39

2014 ◽  
Vol 54 ◽  
pp. 255-265 ◽  
Author(s):  
Jonathan Puente-Rivera ◽  
Lucero de los Ángeles Ramón-Luing ◽  
Elisa Elvira Figueroa-Angulo ◽  
Jaime Ortega-López ◽  
Rossana Arroyo
Keyword(s):  
Trichomonas Vaginalis ◽  
Cysteine Proteinases ◽  
Endogenous Inhibitor

Efficacy of Punica granatum extract on in vitro and in vivo control of Trichomonas vaginalis

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
GT El Sherbini ◽  
KM Ibrahim ◽  
ET El Sherbini ◽  
NM Abdel Hady ◽  
TA Morsy
Keyword(s):  
Trichomonas Vaginalis ◽  
Punica Granatum
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