scholarly journals Differentiation ofLactobacillus sanfranciscensisstrains by randomly amplified polymorphic DNA and pulsed-field gel electrophoresis

1998 ◽  
Vol 166 (2) ◽  
pp. 325-332 ◽  
Author(s):  
Giacomo Zapparoli ◽  
Sandra Torriani ◽  
Franco Dellaglio
Keyword(s):  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Randomly Amplified Polymorphic Dna ◽  
Pulsed Field

Detection and Genotyping of Leuconostoc spp. in a Sausage Processing Plant

2015 ◽  
Vol 78 (12) ◽  
pp. 2170-2176 ◽  
Author(s):  
J. J. PADILLA-FRAUSTO ◽  
L. G. CEPEDA-MARQUEZ ◽  
L. M. SALGADO ◽  
M. H. ITURRIAGA ◽  
S. M. ARVIZU-MEDRANO
Keyword(s):  
Genetic Diversity ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Processing Plant ◽  
Randomly Amplified Polymorphic Dna ◽  
Pulsed Field ◽  
Two Samples ◽  
Conveyor Belts

Some Leuconostoc spp. have the ability to produce slime and undesirable compounds in cooked sausage. The objectives of this research were to identify Leuconostoc sources in a Vienna-type sausage processing plant and to evaluate the genetic diversity of the isolated strains. Three hundred and two samples of sausage batter, sausages during processing, spoiled sausage, equipment surfaces, chilling brine, workers' gloves and aprons, and used casings were collected (March to November 2008 and February to April 2010) from a sausage processing plant. Lactic acid bacteria (LAB) were quantified, and Leuconostoc were detected using PCR. Strains were isolated and identified in Leuconostoc-positive samples. Leuconostoc strains were genotyped using randomly amplified polymorphic DNA and pulsed-field gel electrophoresis. LAB content of nonspoiled and spoiled sausage ranged from <0.8 to 4.4 log CFU/g and from 4.9 to 8.3 log CFU/g, respectively. LAB levels on equipment surfaces ranged from <1.3 to 4.8 log CFU/100 cm2. Leuconostoc was detected in 35% of the samples, and 88 Leuconostoc spp. strains were isolated and genotyped. The main Leuconostoc spp. isolated were L. mesenteroides (37 genotypes), L. fallax (29 genotypes), and L. lactis (6 genotypes). Some strains of Leuconostoc isolated from equipment surfaces and sausages showed the same genotype. One L. lactis genotype included strains isolated from spoiled sausages analyzed in April 2008 and March to April 2010. Equipment and conveyor belts constitute Leuconostoc contamination sources. Leuconostoc persistence in the sausage processing environment and in the final product suggests the existence of microbial reservoirs, possibly on equipment surfaces.

scholarly journals Comparison of Randomly Amplified Polymorphic DNA Analysis and Pulsed-Field Gel Electrophoresis for Typing of Moraxella catarrhalis Strains

1999 ◽  
Vol 37 (2) ◽  
pp. 450-452 ◽  
Author(s):  
Hoang Vu-Thien ◽  
Carole Dulot ◽  
Didier Moissenet ◽  
Brigitte Fauroux ◽  
Antoine Garbarg-Chenon
Keyword(s):  
Gel Electrophoresis ◽  
Moraxella Catarrhalis ◽  
Dna Analysis ◽  
Pulsed Field Gel Electrophoresis ◽  
Randomly Amplified Polymorphic Dna ◽  
Pulsed Field ◽  
Chronic Colonization ◽  
Different Strains

Randomly amplified polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) for the analysis of 13 Moraxella catarrhalis isolates, 11 successive strains isolated from sputa of five children and 2 isolates obtained the same day from twins, were compared. RAPD and PFGE both yielded nine types from the 13 isolates, showing a chronic colonization with one strain in three patients and a successive colonization with different strains in two patients. The promising results obtained with RAPD should be confirmed with a larger number of strains, but RAPD seems as suitable as PFGE for the typing ofM. catarrhalis.

scholarly journals Comparison of Ribotyping, Randomly Amplified Polymorphic DNA Analysis, and Pulsed-Field Gel Electrophoresis in Typing ofLactobacillus rhamnosus and L. caseiStrains

1999 ◽  
Vol 65 (9) ◽  
pp. 3908-3914 ◽  
Author(s):  
Soile Tynkkynen ◽  
Reetta Satokari ◽  
Maria Saarela ◽  
Tiina Mattila-Sandholm ◽  
Maija Saxelin
Keyword(s):  
Gel Electrophoresis ◽  
Type Strain ◽  
Dna Analysis ◽  
Rapd Analysis ◽  
Pulsed Field Gel Electrophoresis ◽  
Strain Atcc ◽  
Randomly Amplified Polymorphic Dna ◽  
Specific Primers ◽  
Pulsed Field ◽  
Species Specific

ABSTRACT A total of 24 strains, biochemically identified as members of theLactobacillus casei group, were identified by PCR with species-specific primers. The same set of strains was typed by randomly amplified polymorphic DNA (RAPD) analysis, ribotyping, and pulsed-field gel electrophoresis (PFGE) in order to compare the discriminatory power of the methods. Species-specific primers for L. rhamnosusand L. casei identified the type strain L. rhamnosus ATCC 7469 and the neotype strain L. caseiATCC 334, respectively, but did not give any signal with the recently revived species L. zeae, which contains the type strain ATCC 15820 and the strain ATCC 393, which was previously classified asL. casei. Our results are in accordance with the suggested new classification of the L. casei group. Altogether, 21 of the 24 strains studied were identified with the species-specific primers. In strain typing, PFGE was the most discriminatory method, revealing 17 genotypes for the 24 strains studied. Ribotyping and RAPD analysis yielded 15 and 12 genotypes, respectively.

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