scholarly journals Multicopy suppression of agacAmutation by theinfCoperon inPseudomonas fluorescensCHA0: competition with the global translational regulator RsmA

2000 ◽  
Vol 187 (1) ◽  
pp. 53-58 ◽  
Author(s):  
Caroline Blumer ◽  
Dieter Haas
Keyword(s):  
Multicopy Suppression

scholarly journals Genetics and Regulation of the Major Enzymes of Alanine Synthesis in Escherichia coli

2010 ◽  
Vol 192 (20) ◽  
pp. 5304-5311 ◽  
Author(s):  
Sok Ho Kim ◽  
Barbara L. Schneider ◽  
Larry Reitzer
Keyword(s):  
Escherichia Coli ◽  
Carbon Source ◽  
Genetic Analysis ◽  
Regulatory Protein ◽  
Carbon Sources ◽  
Optimal Growth ◽  
Expression Studies ◽  
Alanine Synthesis ◽  
Multicopy Suppression

ABSTRACT Genetic analysis of alanine synthesis in the model genetic organism Escherichia coli has implicated avtA, the still uncharacterized alaA and alaB genes, and probably other genes. We identified alaA as yfbQ. We then transferred mutations in several transaminase genes into a yfbQ mutant and isolated a mutant that required alanine for optimal growth. For cells grown with carbon sources other than pyruvate, the major alanine-synthesizing transaminases are AvtA, YfbQ (AlaA), and YfdZ (which we designate AlaC). Growth with pyruvate as the carbon source and multicopy suppression suggest that several other transaminases can contribute to alanine synthesis. Expression studies showed that alanine modestly repressed avtA and yfbQ but had no effect on yfdZ. The leucine-responsive regulatory protein (Lrp) mediated control by alanine. We purified YfbQ and YfdZ and showed that both are dimers with Km s for pyruvate within the intracellular range of pyruvate concentration.

Acquisition of new metabolic capabilities: multicopy suppression by cloned transaminase genes in Escherichia coli K-12

Gene ◽  
1988 ◽  
Vol 65 (2) ◽  
pp. 195-202 ◽  
Author(s):  
Claire M. Berg ◽  
Ming-der Wang ◽  
Narendra B. Vartak ◽  
Lin Liu
Keyword(s):  
Escherichia Coli ◽  
Multicopy Suppression ◽  
K 12

scholarly journals Multicopy Suppression Underpins Metabolic Evolvability

2007 ◽  
Vol 24 (12) ◽  
pp. 2716-2722 ◽  
Author(s):  
W. M. Patrick ◽  
E. M. Quandt ◽  
D. B. Swartzlander ◽  
I. Matsumura
Keyword(s):  
Multicopy Suppression

scholarly journals Multicopy suppression of cold-sensitive sec mutations in Escherichia coli.

1995 ◽  
Vol 177 (17) ◽  
pp. 4969-4973 ◽  
Author(s):  
P N Danese ◽  
C K Murphy ◽  
T J Silhavy
Keyword(s):  
Escherichia Coli ◽  
Cold Sensitive ◽  
Multicopy Suppression

Multicopy Suppression Screen in the msb3 msb4 Saccharomyces cerevisiae Double Mutant, Affected in Ypt/RabGAP Activity

2005 ◽  
Vol 27 (19) ◽  
pp. 1439-1449 ◽  
Author(s):  
Christophe Dechamps ◽  
Daniel Portetelle ◽  
Micheline Vandenbol
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Double Mutant ◽  
Multicopy Suppression
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